Research Supplement for Kennelia Mellanson

摘要 在这里，我们从NIA申请R 01补助金的研究补充，提交它以回应 PA-21-071，旨在促进科学研究队伍的多样性， 合格研究者的职业生涯。我们提出了一个详细的计划，以促进职业生涯的 Kennelia Mellanson，实验室的研究生。Kennelia目前的项目重点是小说 定量方法来确定心室和胶质淋巴系统的关键组成部分， 脑中的运输-纤毛搏动，脑脊液（CSF）的定向流动，以及 脑室管膜层的结构-对有效的心室运输至关重要的特征， CSF和水通道蛋白4（AQP 4）通道的结构，它们是CSF的关键组成部分。 胶质淋巴运输系统 我们的提案与NIA“淋巴和CSF系统”的原始R 01拨款完全一致 在老化和阿尔茨海默氏病”，其中重点是脑组织的稳态机制 以及阿尔茨海默病的清除。我们现在建议将我们的新技术和发现用于心室肌， 和胶质淋巴运输，以确定随着衰老和进展的变化， 阿尔茨海默氏症的小鼠模型，并利用这个项目作为一个平台，帮助和指导 Kennelia Mellanson在发展她的职业生涯作为一个研究员和学者。我们提出了一个详细 Kennelia的专业发展计划，包括详细的计划和指导时间轴， 手稿和赠款写作，教学活动，提高演讲技巧，参与 会议和课程，以及网络。此外，我们提出了一个研究计划，研究的关键， 运输系统的组成部分可能会受到老化和阿尔茨海默病的影响。

ABSTRACT Here we apply for a research supplement to our R01 grant from the NIA, submitting it in response to PA-21-071 which is directed at fostering diversity in scientific research workforce and developing the careers of eligible investigators. We present a detailed plan to advance the professional career of Kennelia Mellanson, a graduate student in the laboratory. Kennelia’s current project focuses on novel quantitative approaches to determine the key components of the ventricular and glymphatic system of transport in the brain – the ciliary beat, the directional flow of the cerebrospinal fluid (CSF), and the structure of the brain ependymal layer – features that are critical for the efficient ventricular transport of the CSF, and the structure of the aquaporin 4 (AQP4) channels, which serve as the key components of the glymphatic system of transport. Our proposal is in full alignment with the original R01 grant from the NIA “Glymphatic and CSF systems in aging and Alzheimer’s disease”, which focuses on the mechanisms of the brain tissue homeostasis and clearance in Alzheimer's. We now propose using our new techniques and findings on the ventricular and glymphatic transport to determine the changes accompany aging and progression of the Alzheimer's disease in a mouse model and to use this project as a platform for helping and directing Kennelia Mellanson in developing her career as a researcher and a scholar. We present a detailed plan for Kennelia’s professional development, including a detailed plan and timeline for mentorship, manuscript and grant writing, didactic activities, improving the presentation skills, participation in meetings and courses, and networking. Furthermore, we present a research plan for studying the key components of the transport systems that may be compromised by aging and by Alzheimer's disease.