Research Supplement for Kennelia Mellanson

肯尼莉亚·梅兰森的研究增刊

• 批准号: 10382622
• 负责人: Helene D Benveniste
• 金额: $ 5.71万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10382622
• 关键词: Age; Aging; Alzheimer' s Disease; Animal Model; Brain; Brain Mass; Cerebral Ventricles; Cerebrospinal Fluid; Complex; Development; Fostering; Grant; Homeostasis; Laboratories; Manuscripts; Mentorship; Nervous system structure; Research; Research Personnel; Structure; System; Techniques; TimeLine; Ventricular; Writing; aquaporin 4; brain health; brain tissue; career; cerebrospinal fluid flow; glymphatic system; graduate student; improved; meetings; mouse model; novel; response; skills; solute; ventricular system; wasting

ABSTRACT Here we apply for a research supplement to our R01 grant from the NIA, submitting it in response to PA-21-071 which is directed at fostering diversity in scientific research workforce and developing the careers of eligible investigators. We present a detailed plan to advance the professional career of Kennelia Mellanson, a graduate student in the laboratory. Kennelia’s current project focuses on novel quantitative approaches to determine the key components of the ventricular and glymphatic system of transport in the brain – the ciliary beat, the directional flow of the cerebrospinal fluid (CSF), and the structure of the brain ependymal layer – features that are critical for the efficient ventricular transport of the CSF, and the structure of the aquaporin 4 (AQP4) channels, which serve as the key components of the glymphatic system of transport. Our proposal is in full alignment with the original R01 grant from the NIA “Glymphatic and CSF systems in aging and Alzheimer’s disease”, which focuses on the mechanisms of the brain tissue homeostasis and clearance in Alzheimer's. We now propose using our new techniques and findings on the ventricular and glymphatic transport to determine the changes accompany aging and progression of the Alzheimer's disease in a mouse model and to use this project as a platform for helping and directing Kennelia Mellanson in developing her career as a researcher and a scholar. We present a detailed plan for Kennelia’s professional development, including a detailed plan and timeline for mentorship, manuscript and grant writing, didactic activities, improving the presentation skills, participation in meetings and courses, and networking. Furthermore, we present a research plan for studying the key components of the transport systems that may be compromised by aging and by Alzheimer's disease.

摘要 在这里，我们从NIA申请R 01补助金的研究补充，提交它以回应 PA-21-071，旨在促进科学研究队伍的多样性， 合格研究者的职业生涯。我们提出了一个详细的计划，以促进职业生涯的 Kennelia Mellanson，实验室的研究生。Kennelia目前的项目重点是小说 定量方法来确定心室和胶质淋巴系统的关键组成部分， 脑中的运输-纤毛搏动，脑脊液（CSF）的定向流动，以及 脑室管膜层的结构-对有效的心室运输至关重要的特征， CSF和水通道蛋白4（AQP 4）通道的结构，它们是CSF的关键组成部分。 胶质淋巴运输系统 我们的提案与NIA“淋巴和CSF系统”的原始R 01拨款完全一致 衰老和阿尔茨海默病”，重点关注脑组织稳态的机制 和清除阿尔茨海默氏症。我们现在建议将我们的新技术和发现用于心室肌， 和胶质淋巴运输，以确定随着衰老和进展的变化， 阿尔茨海默氏症的小鼠模型，并利用这个项目作为一个平台，帮助和指导 Kennelia Mellanson在发展她的职业生涯作为一个研究员和学者。我们提出了一个详细 Kennelia的专业发展计划，包括详细的计划和指导时间轴， 手稿和资助写作、教学活动、提高演讲技巧、参与 会议和课程，以及网络。此外，我们提出了一个研究计划，研究的关键， 运输系统的组成部分可能会受到老化和阿尔茨海默病的影响。

项目成果

Spatiotemporal 3D image registration for mesoscale studies of brain development.

时空3D图像登记，用于脑发育中尺度研究。

• DOI: 10.1038/s41598-022-06871-8
• 发表时间: 2022-03-07
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Shuvaev S;Lazutkin A;Kiryanov R;Anokhin K;Enikolopov G;Koulakov AA
• 通讯作者: Koulakov AA

The bioavailability time of commonly used thymidine analogues after intraperitoneal delivery in mice: labeling kinetics in vivo and clearance from blood serum.

• DOI: 10.1007/s00418-021-02048-y
• 发表时间: 2022-02
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Maltsev, Dmitry I.;Mellanson, Kennelia A.;Belousov, Vsevolod V.;Enikolopov, Grigori N.;Podgorny, Oleg V.
• 通讯作者: Podgorny, Oleg V.

Physiology of Glymphatic Solute Transport and Waste Clearance from the Brain.

• DOI: 10.1152/physiol.00015.2022
• 发表时间: 2022-11-01
• 期刊: Physiology (Bethesda, Md.)
• 作者: Zhao, Lucy;Tannenbaum, Allen;Benveniste, Helene
• 通讯作者: Benveniste, Helene

Age-related decline in cognitive flexibility is associated with the levels of hippocampal neurogenesis.

• DOI: 10.3389/fnins.2023.1232670
• 发表时间: 2023
• 期刊: FRONTIERS IN NEUROSCIENCE
• 影响因子: 4.3
• 作者: Amelchenko, Evgeny M.;Bezriadnov, Dmitri V.;Chekhov, Olga A.;Anokhin, Konstantin V.;Lazutkin, Alexander A.;Enikolopov, Grigori
• 通讯作者: Enikolopov, Grigori

NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.

• DOI: 10.1038/s41598-018-33613-6
• 发表时间: 2018-10-15
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Barykina NV;Doronin DA;Subach OM;Sotskov VP;Plusnin VV;Ivleva OA;Gruzdeva AM;Kunitsyna TA;Ivashkina OI;Lazutkin AA;Malyshev AY;Smirnov IV;Varizhuk AM;Pozmogova GE;Piatkevich KD;Anokhin KV;Enikolopov G;Subach FV
• 通讯作者: Subach FV