KC Donnelly Externship–Promotion of Translational/Transdisciplinary Efforts in Graduate & Post-Doctoral Research

KC Donnelly 实习 — 促进研究生的转化/跨学科努力

• 批准号: 10382018
• 负责人: Sanjay Srivastava
• 金额: $ 1.56万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10382018
• 关键词: 1,3-Butadiene; Air; Arterial Injury; Atherosclerosis; Automobile Exhaust; Butadiene; Cardiopulmonary; Cardiovascular Diseases; Cell Differentiation process; Cell Survival; Cells; Chemicals; Development; Electrical Resistance; Endothelial Cells; Endothelium; Epithelial; Exposure to; Gene Expression; Generations; Goals; Hazardous Waste Sites; Household; Impairment; In Vitro; Industrialization; Inhalation; Injury; Liquid substance; Lung; Nitric Oxide; Petroleum; Population Study; Postdoctoral Fellow; Production; Reactive Oxygen Species; Research; Role; Signal Recognition Particle; Solvents; Superfund; Synthetic Rubber; System; Tobacco smoke; Toxic effect; Universities; cardiovascular injury; cytokine; cytotoxicity; dosimetry; endothelial stem cell; externship; improved; lung injury; repaired; transcriptome sequencing; volatile organic compound

Abstract The overarching goal of my research is to investigate the role of volatile organic compound (VOC) exposure on the development of cardiovascular disease (CVD). Volatile organic compounds are gaseous chemicals present in industrial solvents, petroleum products, automobile exhaust, tobacco smoke, and household chemicals. VOCs are abundant at several Superfund and hazardous waste sites. Population based studies at the University of Louisville SRP show that VOCs, such as 1,3-butadiene (BD), used in the production of synthetic rubber, are positively associated with sub-clinical markers of cardiopulmonary injury (endothelial cell and lung microparticles) and proinflammatory cytokines. These associations are also accompanied by a decrease in endothelial progenitor cells, reflecting an impaired endothelial repair capacity. Studies performed by Dr. Arthur Penn (LSU SRP) have demonstrated that exposure to 1,3- butadiene via inhalation exacerbated atherosclerosis in cockerels.1 It is unclear whether cardiovascular injury of 1,3-butadiene is due to direct effects of the chemical or secondary effects of 1,3-butadiene-induced pulmonary toxicity. We will be assessing the toxicity of BD using a ‘state of the art’ air-liquid interface (ALI) cell exposure system (Vitrocell).

摘要 我的研究的首要目标是研究挥发性有机物的作用 化合物（VOC）暴露对心血管疾病（CVD）的发展。 挥发性有机化合物是存在于工业溶剂中的气态化学品， 石油产品、汽车尾气、烟草烟雾和家用化学品。 挥发性有机化合物在几个超级基金和危险废物场地大量存在。基于人群 路易斯维尔大学SRP的研究表明，VOC，如1，3-丁二烯（BD）， 用于生产合成橡胶，与亚临床 心肺损伤标志物（内皮细胞和肺微粒）， 促炎细胞因子这些关联还伴随着 内皮祖细胞，反映受损的内皮修复能力。研究 由亚瑟佩恩博士（路易斯安那州立大学SRP）进行的研究表明， 通过吸入丁二烯加剧了公鸡的动脉粥样硬化。 1，3-丁二烯的心血管损伤是由于化学物质的直接影响， 1，3-丁二烯诱导的肺毒性的继发效应。我们将评估 使用“最新技术水平”气液界面（ALI）细胞暴露系统的BD毒性 （Vitrocell）。