Neuropathology Core

项目总结-神经病理学核心（核心D） 威斯康星州阿尔茨海默病研究中心（ADRC）神经病理学核心的使命是： 提供阿尔茨海默病（AD）和其他痴呆症患者大脑的详细特征; 促进各种ADRC核心之间的科学和智力互动;并作为一个本地和 国家AD标本库。核心将继续促进临床病理和翻译 通过向威斯康星州提供关于正常衰老、轻度认知障碍（MCI）、AD和其他痴呆症的研究， ADRC和外部研究者提供生物标本、生物标志物数据、临床数据和 受试者MCI和AD、血管、路易体、TDP-43和混合病理的最新诊断 临床核心和威斯康星州阿尔茨海默病预防登记处（WRAP）。神经病理学核心将 通过结合临床数据（核心B）生成和汇集关键诊断信息，创新 神经成像和生物标志物测定（核心G）收集在生活与脑尸检，和形态学， 免疫组织化学、遗传学、细胞和分子死后分析。神经病理学核心还将 提供尖端工具（包括基因组学和蛋白质组学），以加强对银行， 冷冻组织此外，核心在建立诱导多能干细胞方面发挥了领导作用。 （iPSC）库，目的是提供来自AD和对照患者的充分表征的干细胞系， 全国各地的调查人员。核心将通过以下方式实现这些目标：1）收集和归档后， 从死亡个体的多个脑区域中取出的死后冷冻和固定的组织块， CSF、血液和DNA，其在威斯康星州ADRC中登记以及正常老年对照; 2）提供 国家阿尔茨海默氏症协调中心收集的临床核心受试者最先进的尸检诊断 中心（NACC）的神经病理学数据，并将结果提供给家庭，相关临床医生， 研究人员和NACC; 3）分发生前和死后生物标本（脑、CSF、血液 产品和DNA）和神经病理学，遗传学，生物标志物和其他数据，以满足合格的 研究项目，无论是在威斯康星大学麦迪逊分校和国家和国际多中心合作; 4） 进行遗传和生物标志物分析，以支持ADRC和外部研究人员;以及5）提供良好的- 向当地和国家研究人员描述iPSC系。因此，设想了一种服务流程， 威斯康星州ADRC的调查人员和合作者能够获得明确的尸检诊断， 神经病理学数据和来自神经病理学核心的组织以及全面的生物标志物数据 （生物标志物服务）和神经影像学数据（核心G）。最后，核心还将支持先进的分子 生物标记和细胞与分子神经科学可以对组织进行细胞分析 服务

PROJECT SUMMARY - NEUROPATHOLOGY CORE (CORE D) The mission of the Neuropathology Core at the Wisconsin Alzheimer's Disease Research Center (ADRC) is to: provide detailed characterization of brains from patients with Alzheimer's disease (AD) and other dementias; facilitate scientific and intellectual interactions between the various ADRC cores; and serve as a local and national repository for AD specimens. The Core will continue to facilitate clinical-pathologic and translational research on normal aging, mild cognitive impairment (MCI), AD, and other dementias by providing to Wisconsin ADRC and outside investigators a comprehensive resource of biospecimens, biomarker data, clinical data, and state-of-the-art diagnoses for MCI and AD, vascular, Lewy body, TDP-43, and mixed pathologies, from subjects in the Clinical Core and Wisconsin Registry for Alzheimer's Prevention (WRAP). The Neuropathology Core will generate and assemble critical diagnostic information by combining clinical data (Core B), innovative neuroimaging and biomarker assays (Core G) collected during life with brain autopsy, and morphologic, immunohistochemical, genetic, cell and molecular post-mortem analyses. The Neuropathology Core will also provide access to cutting edge tools (including genomics and proteomics) to enhance AD research on banked, frozen tissue. Additionally, the Core has taken a leadership role in setting up an induced pluripotent stem cells (iPSC) bank, with the aim of providing well-characterized stem cell lines from AD and control patients to investigators throughout the country. The Core will accomplish these goals by 1) collecting and archiving post- mortem frozen and fixed tissue blocks from multiple brain regions on deceased individuals, and ante-mortem CSF, blood, and DNA who are enrolled in the Wisconsin ADRC as well as normal elderly controls; 2) providing state-of-the-art postmortem diagnoses on Clinical Core subjects, collect National Alzheimer's Coordinating Center (NACC) neuropathology data and make the results available to the family, relevant clinicians, qualified researchers, and NACC; 3) distributing ante-mortem and post-mortem biospecimens (brain, CSF, blood products, and DNA) and neuropathologic, genetic, biomarker, and other data to suit the requirements of qualified research projects, both within UW-Madison and for national and international multi-center collaborations; 4) performing genetic and biomarker analyses in support of ADRC and outside investigators; and 5) providing well- characterized iPSC lines to local and national researchers. A flow of services is therefore envisaged whereby Wisconsin ADRC investigators and collaborators are able to obtain unambiguous post-mortem diagnosis, neuropathological data, and tissues from the Neuropathology Core together with comprehensive biomarker data (Biomarker Services) and neuroimaging data (Core G). Finally, the Core will also support advanced molecular and cellular analyses can be performed on tissues by the Biomarker and Cellular and Molecular Neuroscience Services.