Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
批准号:
10389836
负责人:
Jennifer S. Brodbelt
金额:
$10.04万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-01-01 至 2025-12-31
关键词:
Antibiotic ResistanceAreaBindingBiologicalBiomedical ResearchC-terminalCapillary ElectrophoresisCellsCellular MembraneChargeChemicalsCollaborationsComplexDiseaseDissociationElectronsGenetic TranscriptionGoalsHybridsIndividualLigandsLipidsMass Spectrum AnalysisMembrane ProteinsMethodsMicrobiologyModificationMolecularMolecular BiologyNucleic AcidsPatternPhosphorylationPost Translational Modification AnalysisPost-Translational Protein ProcessingPreparationProteinsRNA Polymerase IIResearchResearch SupportSamplingSolventsStructureStructure-Activity RelationshipTechnologybasedesigndisulfide bondfrontierfunctional outcomesinnovationinsightlipid Imacromolecular assemblymass spectrometernovel therapeuticsoxidationpathogenic bacteriaprogramsprotein complexprotein functiontandem mass spectrometryultraviolet
中文摘要
摘要。了解脂质,蛋白质,甚至更大的大分子组件的功能
英文摘要
Abstract. Understanding the functions of lipids, proteins and even larger macromolecular assemblies
depends on deciphering complex structures of individual molecules as well as decrypting how those
molecules interact, often via networks of non-covalent interactions. In order to advance the elucidation of
biomolecular organization and functional outcomes, new methods are needed to push the limits of
structural insight, providing more detailed holistic chemical information with greater sensitivity. The critical
interplay between structure/function is evidenced in numerous biologically-motivated problems, ranging
from understanding the ways that pathogenic bacteria develop antibiotic resistance to the design of new
drugs that selectively bind and inhibit the functions of protein targets. The ongoing need for even greater
chemical insight has motivated my group’s effort to develop innovative mass spectrometry methods to
characterize structures of biological molecules in unprecedented detail, especially lipids and proteins
which are featured in this proposal. The overarching goal of my research program is to develop state-of-
the-art tandem mass spectrometry technologies, particularly highlighting ultraviolet photodissociation
(UVPD) and hybrid MS/MS methods, for structural elucidation of lipids, proteins, and protein complexes.
These new methods will be showcased for solving challenging problems in three areas. (1) Lipids: (i)
profiling lipids of pathogenic bacteria and their signatures of antibiotic resistance, and (ii) structural
characterization of unsaturations, oxidations and modifications of lipids that occur during remodeling of
cellular membranes. (2) Protein complexes: (i) characterization of protein-ligand complexes, membrane
protein complexes, protein/nucleic acid complexes, and macromolecular assemblies, and (ii) advancing
capillary electrophoresis for native separations and exploration of the interactome. (3) Post-translational
modifications: focusing on decoding the phosphorylation patterns of the C-terminal domain of RNA
polymerase II which regulates transcription. These high impact problems are supported via numerous
collaborations with microbiology and molecular biology groups who recognize the value of frontier mass
spectrometry strategies for elevating biomedical research. This supplement supports acquisition of an
ExD cell to enable electron-based activation on existing mass spectrometers and a solvent evaporator to
accelerate sample preparation workflows. The ExD cell will support research areas 2 and 3 by allowing
electron capture dissociation and charge reduction capabilities for analysis of post-translational
modifications and cleavage of disulfide bonds in proteins. The solvent evaporator will facilitate
preparation of samples in all three research areas.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
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批准号:10418608
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项目类别:
-
资助金额:$17.03万
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财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
-
批准号:10620850
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项目类别:
-
资助金额:$17.44万
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财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
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批准号:10320024
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项目类别:
-
资助金额:$64.61万
-
财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
-
批准号:10089703
-
项目类别:
-
资助金额:$7.86万
-
财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
-
批准号:10797256
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项目类别:
-
资助金额:$16.0万
-
财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
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批准号:10543449
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项目类别:
-
资助金额:$64.61万
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财政年份:2021
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Interpretation of the phosphorylation code of RNA polymerase II during eukaryotic transcription
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批准号:9751900
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项目类别:
-
资助金额:$34.7万
-
财政年份:2018
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负责人:Jennifer S. Brodbelt
-
依托单位:
Interpretation of the phosphorylation code of RNA polymerase II during eukaryotic transcription
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批准号:10158496
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项目类别:
-
资助金额:$34.7万
-
财政年份:2018
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负责人:Jennifer S. Brodbelt
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依托单位:
UVPD Mass Spectrometry of Protein Complexes
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批准号:9217240
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项目类别:
-
资助金额:$28.47万
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财政年份:2017
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负责人:Jennifer S. Brodbelt
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依托单位:
UVPD Mass Spectrometry of Protein Complexes
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批准号:9539104
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项目类别:
-
资助金额:$3.73万
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财政年份:2017
-
负责人:Jennifer S. Brodbelt
-
依托单位:
Cracking the Ubiquitination Code by Top Down Mass Spectrometry
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批准号:8959461
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项目类别:
-
资助金额:$18.94万
-
财政年份:2015
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负责人:Jennifer S. Brodbelt
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依托单位:
Mapping the C Terminal Domain of RNA Polymerase II by UVPD Mass Spectrometry
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批准号:8676088
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项目类别:
-
资助金额:$21.7万
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财政年份:2014
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负责人:Jennifer S. Brodbelt
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依托单位:
Mapping the C Terminal Domain of RNA Polymerase II by UVPD Mass Spectrometry
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批准号:8806559
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项目类别:
-
资助金额:$18.77万
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财政年份:2014
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负责人:Jennifer S. Brodbelt
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依托单位:
IRACDA Postdoctoral Program: Collaborative Opportunities for Research Educators
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批准号:8697068
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项目类别:
-
资助金额:$43.59万
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财政年份:2013
-
负责人:Jennifer S. Brodbelt
-
依托单位:
IRACDA Postdoctoral Program: Collaborative Opportunities for Research Educators
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批准号:9086376
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项目类别:
-
资助金额:$62.11万
-
财政年份:2013
-
负责人:Jennifer S. Brodbelt
-
依托单位:
IRACDA Postdoctoral Program: Collaborative Opportunities for Research Educators
-
批准号:8550189
-
项目类别:
-
资助金额:$22.83万
-
财政年份:2013
-
负责人:Jennifer S. Brodbelt
-
依托单位:
IRACDA Postdoctoral Program: Collaborative Opportunities for Research Educators
-
批准号:8901205
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项目类别:
-
资助金额:$62.26万
-
财政年份:2013
-
负责人:Jennifer S. Brodbelt
-
依托单位:
IRACDA Postdoctoral Program: Collaborative Opportunities for Research Educators
-
批准号:9297315
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项目类别:
-
资助金额:$33.42万
-
财政年份:2013
-
负责人:Jennifer S. Brodbelt
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依托单位:
Mass Spectrometric Characterization of Lipopolysaccharides
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批准号:8547083
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项目类别:
-
资助金额:$26.85万
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财政年份:2012
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负责人:Jennifer S. Brodbelt
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依托单位:
Mass Spectrometric Characterization of Lipopolysaccharides
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批准号:8438821
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项目类别:
-
资助金额:$30.81万
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财政年份:2012
-
负责人:Jennifer S. Brodbelt
-
依托单位:
国内基金
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批准号:2021JJ40433
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资助金额:24.0万元
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批准年份:2020
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依托单位:
AREA国际经济模型的移植.改进和应用
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依托单位: