Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
基本信息
- 批准号:10543449
- 负责人:
- 金额:$ 64.61万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-01 至 2025-12-31
- 项目状态:未结题
- 来源:
- 关键词:Antibiotic ResistanceAreaBindingBiologicalBiomedical ResearchC-terminalCapillary ElectrophoresisCellular MembraneChemicalsCollaborationsComplexDiseaseGenetic TranscriptionGoalsHybridsIndividualLigandsLipidsMass Spectrum AnalysisMembrane ProteinsMethodsMicrobiologyModificationMolecularMolecular BiologyNucleic AcidsPatternPhosphorylationPost-Translational Protein ProcessingProteinsRNA Polymerase IIResearchStructureTechnologydesignfrontierfunctional outcomesinnovationinsightmacromolecular assemblynovel therapeuticsoxidationpathogenic bacteriaprogramsprotein complexprotein functiontandem mass spectrometryultraviolet
项目摘要
Abstract. Understanding the functions of lipids, proteins and even larger macromolecular assemblies
depends on deciphering complex structures of individual molecules as well as decrypting how those
molecules interact, often via networks of non-covalent interactions. In order to advance the elucidation of
biomolecular organization and functional outcomes, new methods are needed to push the limits of
structural insight, providing more detailed holistic chemical information with greater sensitivity. The critical
interplay between structure/function is evidenced in numerous biologically-motivated problems, ranging
from understanding the ways that pathogenic bacteria develop antibiotic resistance to the design of new
drugs that selectively bind and inhibit the functions of protein targets. The ongoing need for even greater
chemical insight has motivated my group’s effort to develop innovative mass spectrometry methods to
characterize structures of biological molecules in unprecedented detail, especially lipids and proteins
which are featured in this proposal. The overarching goal of my research program is to develop state-of-
the-art tandem mass spectrometry technologies, particularly highlighting ultraviolet photodissociation
(UVPD) and hybrid MS/MS methods, for structural elucidation of lipids, proteins, and protein complexes.
These new methods will be showcased for solving challenging problems in three areas. (1) Lipids: (i)
profiling lipids of pathogenic bacteria and their signatures of antibiotic resistance, and (ii) structural
characterization of unsaturations, oxidations and other modifications of lipids that occur during
remodeling of cellular membranes. (2) Protein complexes: (i) characterization of protein-ligand
complexes, membrane protein complexes, protein/nucleic acid complexes, and macromolecular
assemblies, and (ii) advancing capillary electrophoresis for native separations and exploration of the
interactome. (3) Post-translational modifications: focusing on decoding the phosphorylation patterns of
the C-terminal domain of RNA polymerase II which regulates transcription. These high impact problems
are supported via numerous collaborations with microbiology and molecular biology groups who
recognize the value of frontier mass spectrometry strategies for elevating biomedical research.
抽象的。了解脂质、蛋白质甚至更大的大分子组装体的功能
取决于破译单个分子的复杂结构,以及这些分子是如何
分子通常通过非共价相互作用的网络相互作用。为了进一步阐明
生物分子组织和功能结果,需要新的方法来推动限制,
结构洞察力,提供更详细的整体化学信息,具有更高的灵敏度。临界
结构/功能之间的相互作用在许多生物学动机的问题中得到证明,
从了解致病菌产生抗生素耐药性的方式,到设计新的
选择性结合并抑制蛋白质靶点功能的药物。持续需要更大的
化学洞察力促使我的团队努力开发创新的质谱分析方法,
以前所未有的细节描述生物分子的结构,特别是脂质和蛋白质
这是本提案中的特色。我的研究计划的首要目标是发展国家的-
最先进的串联质谱技术,特别强调紫外光解离
(UVPD)和混合MS/MS方法,用于脂质、蛋白质和蛋白质复合物的结构解析。
这些新方法将被展示用于解决三个领域的挑战性问题。(1)脂质:(i)
分析病原菌的脂质及其抗生素抗性的特征,和(ii)结构
表征过程中发生的脂质的不饱和、氧化和其他修饰
细胞膜的重塑。(2)蛋白质复合物:(i)蛋白质-配体的表征
复合物、膜蛋白复合物、蛋白/核酸复合物和大分子
组装,以及(ii)推进毛细管电泳用于天然分离和探索
相互作用体(3)翻译后修饰:专注于解码的磷酸化模式,
调节转录的RNA聚合酶II的C末端结构域。这些高影响力的问题
通过与微生物学和分子生物学小组的大量合作得到支持,
认识到前沿质谱分析战略对提升生物医学研究的价值。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jennifer S. Brodbelt其他文献
Effects of functional group interactions on the bimolecular and dissociation reactions of diols
- DOI:
10.1016/1044-0305(92)85030-n - 发表时间:
1992-07-01 - 期刊:
- 影响因子:
- 作者:
Erika S. Eichmann;Erwin Alvarez;Jennifer S. Brodbelt - 通讯作者:
Jennifer S. Brodbelt
Evaluation of steric and substituent effects in phenols by competitive reactions of dimethyl ether ions in a quadrupole ion trap
- DOI:
10.1016/s1044-0305(05)80051-0 - 发表时间:
1995-08-01 - 期刊:
- 影响因子:
- 作者:
Gerald F. Bauerle;Jennifer S. Brodbelt - 通讯作者:
Jennifer S. Brodbelt
Tracking molecular mobility
追踪分子迁移率
- DOI:
10.1038/nchem.382 - 发表时间:
2009-10-01 - 期刊:
- 影响因子:20.200
- 作者:
Jennifer S. Brodbelt - 通讯作者:
Jennifer S. Brodbelt
Ultraviolet photoactivation perturbs the metal-ligand interface of atomically precise nanoclusters
紫外光活化干扰了原子精确纳米团簇的金属-配体界面
- DOI:
10.1039/d5cc00757g - 发表时间:
2025-03-27 - 期刊:
- 影响因子:4.200
- 作者:
Anagha Jose;Jada N. Walker;Maya Khatun;Sami Malola;B. S. Sooraj;Hannu Häkkinen;Jennifer S. Brodbelt;Thalappil Pradeep - 通讯作者:
Thalappil Pradeep
Correlation between Spectroscopic and Structural Features of Dimeric DNA-Templated Silver Nanoclusters using Mass Spectrometry
- DOI:
10.1016/j.bpj.2020.11.1731 - 发表时间:
2021-02-12 - 期刊:
- 影响因子:
- 作者:
Soonwoo Hong;Ines C. Santos;Yu-An Kuo;Yuan-I Chen;Trung D. Nguyen;Hsin-Chin Li;Pranav Anbarasu;Jennifer S. Brodbelt;Tim Yeh - 通讯作者:
Tim Yeh
Jennifer S. Brodbelt的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jennifer S. Brodbelt', 18)}}的其他基金
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
问题到产品团队创业与主动指导(P2P-TEAM)研究生培训计划
- 批准号:
10418608 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
问题到产品团队创业与主动指导(P2P-TEAM)研究生培训计划
- 批准号:
10620850 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
- 批准号:
10389836 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
- 批准号:
10320024 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Problem-to-Product Team Entrepreneurship and Active Mentoring (P2P-TEAM) Graduate Training Program
问题到产品团队创业与主动指导(P2P-TEAM)研究生培训计划
- 批准号:
10089703 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
- 批准号:
10797256 - 财政年份:2021
- 资助金额:
$ 64.61万 - 项目类别:
Interpretation of the phosphorylation code of RNA polymerase II during eukaryotic transcription
真核转录过程中RNA聚合酶II磷酸化密码的解读
- 批准号:
9751900 - 财政年份:2018
- 资助金额:
$ 64.61万 - 项目类别:
Interpretation of the phosphorylation code of RNA polymerase II during eukaryotic transcription
真核转录过程中RNA聚合酶II磷酸化密码的解读
- 批准号:
10158496 - 财政年份:2018
- 资助金额:
$ 64.61万 - 项目类别:
UVPD Mass Spectrometry of Protein Complexes
蛋白质复合物的 UVPD 质谱分析
- 批准号:
9217240 - 财政年份:2017
- 资助金额:
$ 64.61万 - 项目类别:
UVPD Mass Spectrometry of Protein Complexes
蛋白质复合物的 UVPD 质谱分析
- 批准号:
9539104 - 财政年份:2017
- 资助金额:
$ 64.61万 - 项目类别:
相似国自然基金
层出镰刀菌氮代谢调控因子AreA 介导伏马菌素 FB1 生物合成的作用机理
- 批准号:2021JJ40433
- 批准年份:2021
- 资助金额:0.0 万元
- 项目类别:省市级项目
寄主诱导梢腐病菌AreA和CYP51基因沉默增强甘蔗抗病性机制解析
- 批准号:32001603
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
AREA国际经济模型的移植.改进和应用
- 批准号:18870435
- 批准年份:1988
- 资助金额:2.0 万元
- 项目类别:面上项目
相似海外基金
Onboarding Rural Area Mathematics and Physical Science Scholars
农村地区数学和物理科学学者的入职
- 批准号:
2322614 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant
TRACK-UK: Synthesized Census and Small Area Statistics for Transport and Energy
TRACK-UK:交通和能源综合人口普查和小区域统计
- 批准号:
ES/Z50290X/1 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Research Grant
Wide-area low-cost sustainable ocean temperature and velocity structure extraction using distributed fibre optic sensing within legacy seafloor cables
使用传统海底电缆中的分布式光纤传感进行广域低成本可持续海洋温度和速度结构提取
- 批准号:
NE/Y003365/1 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Research Grant
Point-scanning confocal with area detector
点扫描共焦与区域检测器
- 批准号:
534092360 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Major Research Instrumentation
Collaborative Research: Scalable Manufacturing of Large-Area Thin Films of Metal-Organic Frameworks for Separations Applications
合作研究:用于分离应用的大面积金属有机框架薄膜的可扩展制造
- 批准号:
2326714 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant
Collaborative Research: Scalable Manufacturing of Large-Area Thin Films of Metal-Organic Frameworks for Separations Applications
合作研究:用于分离应用的大面积金属有机框架薄膜的可扩展制造
- 批准号:
2326713 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant
Unlicensed Low-Power Wide Area Networks for Location-based Services
用于基于位置的服务的免许可低功耗广域网
- 批准号:
24K20765 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
RAPID: Collaborative Research: Multifaceted Data Collection on the Aftermath of the March 26, 2024 Francis Scott Key Bridge Collapse in the DC-Maryland-Virginia Area
RAPID:协作研究:2024 年 3 月 26 日 DC-马里兰-弗吉尼亚地区 Francis Scott Key 大桥倒塌事故后果的多方面数据收集
- 批准号:
2427233 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant
Postdoctoral Fellowship: OPP-PRF: Tracking Long-Term Changes in Lake Area across the Arctic
博士后奖学金:OPP-PRF:追踪北极地区湖泊面积的长期变化
- 批准号:
2317873 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant
RAPID: Collaborative Research: Multifaceted Data Collection on the Aftermath of the March 26, 2024 Francis Scott Key Bridge Collapse in the DC-Maryland-Virginia Area
RAPID:协作研究:2024 年 3 月 26 日 DC-马里兰-弗吉尼亚地区 Francis Scott Key 大桥倒塌事故后果的多方面数据收集
- 批准号:
2427232 - 财政年份:2024
- 资助金额:
$ 64.61万 - 项目类别:
Standard Grant