Resource for native mass spectrometry guided structural biology-Refeyn OneMP Mass Photometer

原生质谱引导结构生物学资源 -Refeyn OneMP 质量光度计

• 批准号: 10400442
• 负责人: Vicki H. Wysocki
• 金额: $ 13.6万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400442
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein; Automobile Driving; Back; Behavior; Biomedical Technology; Buffers; Cells; Chemicals; Collaborations; Complement; Complex; Core Facility; Country; Coupled; Cryoelectron Microscopy; DNA; Data; Devices; Dissociation; Ebola virus; Electrolytes; Equipment; Heterogeneity; Imaging Device; Immunology; Institutes; Interferometry; Kinetics; Laboratories; Light; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Mission; Modeling; Molecular Chaperones; Molecular Conformation; Monitor; Nucleoproteins; Organism; Peptides; Performance; Photometry; Polymorph; Protein Engineering; Proteins; RNA; RNase P; Rapid screening; Research; Research Personnel; Resolution; Resources; Running; Sampling; Scheme; Services; Structure; Surface; System; Technology; Time; Training; United States National Institutes of Health; Universities; Washington; Work; aggregation pathway; base; computerized tools; equipment acquisition; instrument; ion mobility; member; multicatalytic endopeptidase complex; nanodisk; new technology; novel; protein complex; rho; single molecule; structural biology; tandem mass spectrometry; tau Proteins; tau aggregation; technology research and development; tool

The P41 Resource for Native Mass Spectrometry Guided Structural Biology (nMS->SB Resource), established in 2018, is a national Biomedical Technology Research Resource that seeks to develop and disseminate novel research technologies and methods for structural biology. The Resource is developing and disseminating a suite of technologies under five Technology Research and Development (TR&D) projects; these include (TR&D 1-3) novel surface-induced dissociation (SID) cells coupled to high-resolution ion mobility (IM) and high-resolution mass spectrometry (HRMS), (TR&D 4) online purification and separation schemes, and (TR&D 5) computational tools for structure determination from experimental nMS data. The Resource has successfully supported the efforts of numerous biomedical researchers across the country and the globe to characterize a variety of noncovalent protein and RNA/DNA:protein complexes, including synthetic amyloid beta (Aβ) and tau aggregates, novel de novo designed protein complexes from the Baker laboratory (University of Washington), orthologous 20S proteasomes expressed by the Sharon laboratory (Weizmann Institute) and recently Ebola virus matrix protein VP40 (La Jolla Institute of Immunology). Our protein:protein and nucleoprotein complexes are typically run at mass spectrometry-friendly concentrations and in mass spectrometry-compatible buffers/ electrolytes. Acquisition of an instrument (Refeyn OneMP Mass Photometer) for light-based mass photometry (interferometry) measurements will provide new technology for our DBP and C&S investigators, allowing us to assess small sample volumes at very low concentrations (pM to low nM). Mass photometry (MP) will provide a quick screen of sample quality and oligomeric distributions present in the sample in the investigator’s provided storage buffer, allowing MS team members to decide on the appropriate next steps for the sample. We will determine whether the sample will go back to the investigator for improvements or whether it is ready for higher resolution, higher mass accuracy native mass spectrometry measurements. For a wide variety of protein complex types, provided by a broad set of DBP and C&S projects, we will establish complex types and concentration behaviors for which the MP and nMS results are duplicative and those for which the two sets of results provide additive/complementary data to characterize the complex more fully over a large range of conditions (oligomerization time/kinetics, concentrations, buffer conditions).

用于天然质谱引导结构生物学的P41资源（nMS->SB资源）， 成立于2018年，是一个国家生物医学技术研究资源，旨在开发和 传播结构生物学的新研究技术和方法。资源正在开发， 通过五个技术研究和发展项目推广一套技术;这些项目 包括（TR&D 1-3）与高分辨率离子迁移率（IM）耦合新型表面诱导解离（SID）单元， 和高分辨率质谱（HRMS），（TR&D 4）在线纯化和分离方案，以及 (TR&D 5）用于从实验nMS数据确定结构的计算工具。资源具有 成功地支持了全国和地球仪众多生物医学研究人员的努力， 表征各种非共价蛋白质和RNA/DNA：蛋白质复合物，包括合成淀粉样蛋白β （Aβ）和tau聚集体，来自Baker实验室（University of 华盛顿），由Sharon实验室（Weizmann研究所）表达的orthosterone 20 S蛋白酶体， 最近埃博拉病毒基质蛋白VP 40（拉霍亚免疫学研究所）。我们的蛋白质：蛋白质和 核蛋白复合物通常在质谱友好的浓度下运行， 光谱兼容缓冲液/电解质。获取仪器（Refeyn OneMP质谱光度计） 基于光的质量测光（干涉）测量将为我们的DBP提供新技术， C&S研究人员，使我们能够在非常低的浓度（pM至低nM）下评估小样品体积。质量 光度法（MP）将提供样品质量和样品中存在的低聚物分布的快速筛选 在研究者提供的存储缓冲区中，允许MS团队成员决定适当的后续步骤 为了样本。我们将确定样本是否将返回给研究者进行改进， 是否准备好进行更高分辨率、更高质量准确度的天然质谱测量。用于 各种各样的蛋白质复合物类型，由广泛的DBP和C&S项目提供，我们将建立 MP和nMS结果重复的复杂类型和浓度行为， 这两组结果提供了加性/互补数据，以更充分地表征复合物， 大范围的条件（低聚时间/动力学、浓度、缓冲条件）。