Acquisition of a Nanoflow Ion Trap Mass Spectrometer

描述（由申请人提供）：该提案要求一个Thermo Finnigan ProteomeX DECAP-51000集成工作站，一个配备LC泵的离子阱质谱仪，一个10端口开关阀，用于多维色谱的强阳离子和反相柱，以及一个纳米喷雾探针。该仪器将满足亚利桑那大学生物科学研究人员的需求。该仪器的主要用户和应用有：(1)Samuel Ward，分子和细胞生物学，线虫细胞分化过程中的信号通路研究，与阿尔茨海默氏症和肌肉萎缩症相关的人类疾病基因同源的基因；(2) Brian Larkins，农业与生命科学学院植物科学，提高玉米蛋白质营养品质所需蛋白质的鉴定与分析；(3) M. Halonen/D。维切里/ F。马丁内斯/ M。Cusanovich，呼吸科学中心，结合免疫球蛋白G4种系启动子、IL-13启动子和cd14启动子调控元件的转录因子；(4) Elizabeth Vierling，分子与细胞生物学，分子伴侣功能；胞质细胞器和热休克蛋白的表达和功能，对正常细胞功能至关重要；(5) Carol Dieckmann，生物化学，衣藻眼斑主要多肽编码基因的突变鉴定；(6) Thomas Baldwin，生物化学，脉冲烷基化质谱研究细菌荧光素酶蛋白折叠；(7) Vicki Wysocki, Chemistry, Mechanisms and Energetics of Peptide Dissociation，这项工作直接适用于生物有机体蛋白质的鉴定。现代蛋白质研究离不开质谱分析。使用专用的微流lc -质谱仪和纳米探针来表征当前质谱设备仪器无法分析的样品，对于这些项目的最大生产力和成功至关重要。该大学对该项目作出了强有力的承诺，包括为位于生物科学部的一个新的“分支”质谱实验室翻新空间，雇用一名全职博士生物质谱专家（每年约6万美元），为蛋白质组学主任（每年约8万美元）和一名技术人员（每年约3.5万美元）提供资金，以帮助进行样品制备，并提供75000美元的费用分摊。

DESCRIPTION (provided by applicant): This proposal requests a Thermo Finnigan ProteomeX DECAP-51000 Integrated Workstation, an ion trap mass spectrometer equipped with LC pumps, a 10 port switching valve, strong cation and reversed phase columns for multidimensional chromatography, and a nanospray probe. This instrument will serve the needs of a number of University of Arizona bioscience researchers. The major users and their applications for the instrumentation are (1) Samuel Ward, Molecular and Cellular Biology, Study of signaling pathways during cell differentiation in the Nematode C.elegans, genes are homologeous to human disease genes linked to Alzheimer's and muscular dystrophy; (2) Brian Larkins, Plant Sciences, College of Agriculture and Life Sciences, Identificationand Analysis of Proteins Required for Improved Maize Protein Nutritional Quality; (3) M. Halonen/D. Vercelli/F. Martinez/M. Cusanovich, Center for Respiratory Sciences, Transcription Factors that Bind Regulatory Elements in the Immunoglobulin G4 Germline Promoter, the IL-13 Promoter, and the CD 14 Promoter; Cellular and Molecular Mechanisms of Asthma (4) Elizabeth Vierling, Molecular and Cellular Biology, Molecular chaperone function; expression and function of cytoplasmic organelle and heat shock proteins, the pathways studied are critical to normal cell function; (5) Carol Dieckmann, Biochemistry, Identification of Mutations in Genes Coding for Major Polypeptides in the Chlamydomonas Eyespot;, (6) Thomas Baldwin, Biochemistry, Pulsed Alkylation MS to Investigate Protein Folding in Bacterial Luciferase; (7) Vicki Wysocki, Chemistry, Mechanisms and Energetics of Peptide Dissociation, this work is directly applicable to the identification of proteins from biological organisms. Modern protein research cannot be accomplished without mass spectrometry. The access to a dedicated microflow LC-mass spectrometer with a nanospray probe to characterize samples that are not amenable to analysis with the current mass spectrometry facility instruments is critical to the maximum productivity and success of these projects. The University has made a strong commitment to the project by by renovating space for a new "branch" mass spectrometry laboratory that is located in Biosciences, by hiring a full time Ph.D. biological mass spectrometry specialist (about $60,000 per year), by providing funds for a Director of Proteomics (about $80,000/year) and a technician (about $35,000/year) to help with sample preparation, and by providing cost sharing in the amount of $75,000.