Acquisition of a Linear Trap-Fourier Transform Mass Spectrometer

描述（由申请人提供）： 亚利桑那大学计划购买ThermoFinnigan LTQ-FT高性能质谱仪（MS）。该仪器将放置在UA质谱设施中，并由MS设施和蛋白质组学设施的工作人员操作。该仪器的主要吸引人的特点是，它结合了线性离子阱（LTQ）的高通量性能与离子回旋共振（FTMS）的高分辨率和高质量精度的HPLC时标。FTMS分辨率为100，000 - 500，000，质量精度为1-2 ppm，LTQ的灵敏度在亚fmol范围内。碰撞诱导解离和电子捕获解离的碎裂是可能的，并提供互补的碎裂信息。红外多光子解离可以提供类似于CID的信息，但是具有激活碎片离子以及前体离子的可能性，并且可以用于预热离子以用于ECD。已经确定了一个主要的用户群体，他们已经使用质谱法来解决生物或医学问题，但由于无法使用LTQ-FT等更高性能的仪器，他们在研究中面临限制。这些包括来自生物化学和分子生物物理学（Vierling，Little），药理学和毒理学（Lau，Monks和Vaillancourt），亚利桑那州癌症中心（Gerner），化学（Wysocki）和VA医院（Galgiani）的谷热卓越中心的教师。这些研究人员将使用拟议的LTQ-FT MS解决的问题类型分为：（1）涉及特定蛋白质和蛋白质复合物的详细结构研究的问题，（2）翻译后修饰的鉴定，以及（3）不涉及蛋白质（药物，藜芦醇）的结构表征问题。

DESCRIPTION (provided by applicant): The University of Arizona proposes to acquire a ThermoFinnigan LTQ-FT high performance mass spectrometer (MS). This instrument will be placed in the UA Mass Spectrometry Facility and operated by staff of the MS facility and the proteomics facilities. This instrument's main attractive features are that it combines linear ion trap (LTQ) high throughput performance on an HPLC timescale with ion cyclotron resonance (FTMS) high resolution and high mass accuracy. FTMS resolution of 100,000-500,000 provides mass accuracy of 1-2 ppm, and the LTQ provides sensitivity in the sub-fmol range. Fragmentation by collision-induced dissociation and electron capture dissociation are possible and provide complementary fragmentation information. Infrared multiphoton dissociation can provide information similar to CID, but with the possibility of activating fragment ions as well as precursor ions and can be used to pre-heat ions for ECD. A major user group that already uses mass spectrometry to solve biological or medical problems, but who have faced limitations in their research because they do not have access to a higher performance instrument such as the LTQ-FT, has been identified. These include faculty from Biochemistry and Molecular Biophysics (Vierling, Little), Pharmacology and Toxicology (Lau, Monks, and Vaillancourt), the Arizona Cancer Center (Gerner), Chemistry (Wysocki), and the Valley Fever Center for Excellence, VA Hospital (Galgiani). The types of problems that will be solved for these investigators with the proposed LTQ-FT MS are grouped into (1) those involving detailed structural studies of specific proteins and protein complexes, (2) identification of post-translational modifications, and (3) structural characterization problems not involving proteins (drugs, prostaglandins)