Acquisition of a Linear Trap-Fourier Transform Mass Spectrometer

描述(由申请人提供)： 亚利桑那大学计划收购ThermoFinnigan LTQ-FT高性能质谱仪(MS)。该仪器将放置在UA质谱学设施中，并由MS设施和蛋白质组学设施的工作人员操作。该仪器的主要特点是结合了线性离子陷阱(LTQ)和离子回旋共振(FTMS)的高分辨率和高质量精度。FTMS分辨率为100,000-500,000，提供1-2 ppm的质量精度，LTQ提供亚fmol范围的灵敏度。通过碰撞诱导解离和电子俘获解离的碎裂是可能的，并提供互补的碎裂信息。红外多光子解离可以提供类似于CID的信息，但具有激活碎片离子和前体离子的可能性，并可用于预热ECD的离子。已经确定了一个主要用户群体，他们已经使用质谱学来解决生物或医学问题，但由于无法获得LTQ-FT等更高性能的仪器，他们在研究中面临限制。他们包括来自生物化学和分子生物物理学(Vierling，Little)、药理学和毒理学(Lau、Monks和Vaillancourt)、亚利桑那州癌症中心(Gerner)、化学(Wysocki)和退伍军人事务部(VA)硅谷发烧卓越中心(Galgiani)的教员。利用拟议的LTQ-FT MS将为这些研究人员解决的问题类型分为(1)涉及特定蛋白质和蛋白质复合体的详细结构研究的问题，(2)识别翻译后修饰的问题，以及(3)不涉及蛋白质(药物、前列腺素)的结构表征问题

DESCRIPTION (provided by applicant): The University of Arizona proposes to acquire a ThermoFinnigan LTQ-FT high performance mass spectrometer (MS). This instrument will be placed in the UA Mass Spectrometry Facility and operated by staff of the MS facility and the proteomics facilities. This instrument's main attractive features are that it combines linear ion trap (LTQ) high throughput performance on an HPLC timescale with ion cyclotron resonance (FTMS) high resolution and high mass accuracy. FTMS resolution of 100,000-500,000 provides mass accuracy of 1-2 ppm, and the LTQ provides sensitivity in the sub-fmol range. Fragmentation by collision-induced dissociation and electron capture dissociation are possible and provide complementary fragmentation information. Infrared multiphoton dissociation can provide information similar to CID, but with the possibility of activating fragment ions as well as precursor ions and can be used to pre-heat ions for ECD. A major user group that already uses mass spectrometry to solve biological or medical problems, but who have faced limitations in their research because they do not have access to a higher performance instrument such as the LTQ-FT, has been identified. These include faculty from Biochemistry and Molecular Biophysics (Vierling, Little), Pharmacology and Toxicology (Lau, Monks, and Vaillancourt), the Arizona Cancer Center (Gerner), Chemistry (Wysocki), and the Valley Fever Center for Excellence, VA Hospital (Galgiani). The types of problems that will be solved for these investigators with the proposed LTQ-FT MS are grouped into (1) those involving detailed structural studies of specific proteins and protein complexes, (2) identification of post-translational modifications, and (3) structural characterization problems not involving proteins (drugs, prostaglandins)