15 Tesla Bruker solariX FTICR MS

描述(由申请人提供)：俄亥俄州立大学建议购买一台Bruker SolariX 15特斯拉傅里叶变换离子回旋共振质谱仪(FTICR MS)。这种多功能的高端仪器将放置在俄亥俄州立大学化学仪器中心(CCIC)内的俄亥俄州立大学质谱学和蛋白质组学设施(MS&P)，这是一个位于俄亥俄州立大学研究办公室的全校中心。该仪器将由MS和蛋白质组学设施的工作人员操作，并从质谱学和主要用户研究小组中挑选训练有素的人员。该仪器的主要吸引人的特点是其广泛的应用，与超高分辨率和高质量精度相结合的双MALDI和ESI源。建议的FTICR MS提供高达40,000,000的分辨率、低于ppm的质量精度和低至100amol范围的灵敏度。外部碰撞细胞中的碰撞诱导解离和电子转移解离(ETD)以及ICR细胞中的持续非共振照射(SORI)和电子转移解离是可能的，并提供了补充的碎裂信息。CASI(连续积累的选定离子)可以通过捕获碰撞细胞来丰富非常低的丰度物种，甚至可以从组织成像中进行。已经确定了一个主要用户群体，他们已经使用质谱学来解决生物医学问题，但由于无法获得更高性能的仪器，如solariX，他们在研究中面临限制。这些教师来自医学院(Torrelles、Rovin、Freitas、Parthun)、综合癌症中心(克林顿)、化学和生物化学(布鲁塞尔、福斯特、Musier-Forsyth和Turro)和物理(Poirier和钟)。这些研究人员将解决的问题类型以及他们将使用的SolariX FTICR MS技术，包括(1)高分辨率MALDI和MALDI组织成像，(2)特定组蛋白和翻译后修饰蛋白质的详细高端、自上而下的结构研究，(3)天然质谱学测量，以获得蛋白质复合体的化学计量和拓扑结构，以及(4)用于代谢物和金属-DNA复合体结构表征的复杂混合物分析。CCIC MS&P设施是一个成熟的、在大学范围内得到充分利用的核心。它为高端仪器提供了最佳的环境--一个充满活力的研究社区，非常需要这样的仪器，以及俄亥俄州立大学的教职员工，他们有热情和专业知识来最大限度地利用solariX。如果获得资金，SolariX将对非常广泛的研究人员群体具有无价的价值。

DESCRIPTION (provided by applicant): The Ohio State University proposes to acquire a Bruker solariX 15 tesla Fourier Transform Ion Cyclotron Resonance mass spectrometer (FTICR MS). This versatile high-end instrument will be placed in the OSU Mass Spectrometry and Proteomics Facility (MS&P) within the Campus Chemical Instrument Center (CCIC), a campus-wide Center that is administratively housed in the OSU Office of Research. The instrument will be operated by staff of the MS and proteomics facility and selected highly trained personnel from mass spectrometry and major user research groups. This instrument's main attractive features are its breadth of application, with a dual MALDI and ESI source coupled to ultrahigh resolution and high mass accuracy. The proposed FTICR MS provides resolution of up to 40,000,000, sub ppm mass accuracy, and sensitivity down to the 100 amol range. Fragmentation by collision-induced dissociation and electron transfer dissociation (ETD), in the external collision cell, and sustained off-resonance irradiation (SORI) and electron transfer dissociation in the ICR cell are possible and provide complementary fragmentation information. CASI (Continuous Accumulation of Selected Ions) is available to enrich very low abundant species by trapping in the collision cell, even from tissue imaging. A major user group that already uses mass spectrometry to solve biomedical problems, but that have faced limitations in their research because they do not have access to a higher performance instrument such as the solariX, has been identified. These include faculty from the College of Medicine (Torrelles, Rovin, Freitas, Parthun), the Comprehensive Cancer Center (Clinton), Chemistry and Biochemistry (Bruschweiler, Foster, Musier-Forsyth, and Turro), and Physics (Poirier and Zhong). The types of problems that will be solved for these investigators, and techniques that they will use with the proposed solariX FTICR MS, include (1) high resolution MALDI and MALDI imaging of tissue, (2) detailed high end, top down structural studies of specific histone proteins and post-translationally-modified proteins, (3) native mass spectrometry measurements to gain stoichiometry and topology of protein complexes, and (4) complex mixture analysis for structural characterization of metabolites and metal-DNA complexes. The CCIC MS&P Facility is an established, well utilized university-wide core. It provides an optimum environment for a high end instrument - a vigorous research community with great need for such an instrument and OSU faculty and staff with the enthusiasm and expertise to use the solariX to its greatest potential. If funded, the solariX will be invaluable to a very broad constituency of researchers.