Protein and PCR Mass Spectrometry

蛋白质和 PCR 质谱分析

• 批准号: 8260270
• 负责人: Vicki H. Wysocki
• 金额: $ 18.74万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8260270
• 关键词: Antibiotic Resistance; Antibodies; Antigens; Bacteriophages; Biological Assay; Biological Markers; Biosensor; Blood; Blood Proteins; Burkholderia; Cell Wall; Coccidioides; Collaborations; Coupled; Deer; Development; Device or Instrument Development; Diagnostic; Diagnostic tests; Emerging Communicable Diseases; Enzyme-Linked Immunosorbent Assay; Equine mule; Extracellular Protein; Fiber; Fluorescence; Genes; Human; Infectious Diseases Research; Label; Larva; Libraries; Ligands; Lung; Mass Spectrum Analysis; Mitochondrial DNA; Mus; Nymph; Organism; Organism Strains; Pilum; Proteins; Proteomics; Research; Research Personnel; Sampling; Serum; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; Tail; Ticks; Toxin; Urine; Vaccines; Vertebrates; Virulence; Virulence Factors; Virulent; assay development; base; biodefense; comparative; design; disease transmission; feeding; mass spectrometer; mutant; pathogen; protein complex; protein expression; research study; tool; vaccine candidate; vector

The PSWRCE Protein and PCR MS Core will collaborate on the following projects: Specific Aim i - Proteomics Analyses for Burkholderia, Coccidioides, and Reservoir Vaccines Projects ¿ Identify protein biomarkers for use as diagnostic or vaccine candidates (from serum, urine, and lung homogenates, with depletion of abundant mouse/human proteins as necessary) ¿ Perform comparative proteomics on pre-fractionated samples (e.g., extracellular proteins or cell wall proteins of two different strains of an organism, or wild type vs. mutant) ¿ Build "blood meal" protein library to use to identify host vertebrate proteins in tick nymphs Specific Aim 2 - Development of Diagnostic Assays ¿ Use biomarker IDs of Sp. Aim i to develop bioaffinity MS for pathogen identification ¿ Use MS to guide development of targeted ELISA or other fluorescence assays (characterize expressed protein antigens, antibodies, and labeled antibodies by MS throughout development of assay) ¿ Compare antibodies vs. small ligands for capture of antigens in bioaffinity MS Specific Aim 3 - Ibis TSOOO PCR-MS Biosensor Applied to Reservoir Vaccines, Burkholderia & Coccidioides ¿ PCR MS (on mitochondrial DNA) for ID of vertebrate host on which vector has fed (e.g., tick nymph fed on mule deer) and ID of tick-borne pathogenic organism present ¿ PCR MS for rapid ID and strain typing of pathogens, as well as confirmation of the presence of toxin genes, antibiotic resistance genes, selected virulence factor genes Specific Aim 4 - Characterization of Protein Complexes (uncompensated synergistic activity) ¿ Structurally characterize large protein-protein complexes (e.g., pili involved in virulence, assemblies of bacteriophage tail fiber proteins useful for biosensors) using a one-of-a-kind modified QTOF MS The antigen discovery coupled to support of diagnostic assay development, PCR-MS, bioaffinity MS, and structural characterization of large protein-protein complexes are unique capabilities of the core.

PSWRCE蛋白和PCR MS Core将在以下项目上合作：