Administration and Management of the Resource for Native MS Guided Structural Biology

本地 MS 引导结构生物学资源的管理和管理

• 批准号: 10441399
• 负责人: Vicki H. Wysocki
• 金额: $ 3.7万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10441399
• 关键词: Advisory Committees; Architecture; Binding; Biochemical; Biological Process; Budgets; Collaborations; Communication; Communities; Data; Devices; Disease; Dissociation; Educational workshop; Ensure; Evaluation; Fostering; Goals; Heterogeneity; International; Lead; Leadership; Lipids; Mass Spectrum Analysis; Measures; Membrane Proteins; Mission; Monitor; New Acceptors; Policies; Procedures; Progress Reports; Proteins; Protocols documentation; Publications; RNA; Research Personnel; Resolution; Resources; Safety; Sampling; Schedule; Science; Services; Specialist; Structure; Surface; Technology; Training; United States National Institutes of Health; Universities; Vendor; Vision; Work; commercialization; community engagement; effectiveness evaluation; graduate student; instrument; ion mobility; meetings; organizational structure; programs; protein complex; sound; stoichiometry; structural biology; tool

Project Summary: Administration and Management. The proposed Resource for Native Mass Spectrometry Guided Structural Biology ( ) brings together a team of outstanding scientific nMSSB Resource leaders who, with the support of select staff, postdoctoral researchers, and graduate students, are well-equipped to collaboratively advance the proposed science, dissemination, and training. This team will work together to produce an integrated workflow for structural characterization of protein:protein, membrane protein:lipid, and RNA:protein complexes. The proposed characterization workflow will lead to a better understanding of how protein complexes carry out their normal biological functions, how alterations in architecture lead to disease, and how to build synthetic mimics to carry out desired biomedical applications. Through integration of all components of the nMSSB Resource (TR&Ds, DBPs, and Community Engagement), we will steer conversion of the developed native MS workflow from an appealing technology used by specialists and their collaborators to a widespread frontline tool that can be used to guide other biochemical and structural tools. The overall goal of the Administration and Management Core is to ensure that the vision of the Resource for Native Mass Spectrometry Guided Structural Biology, nMS  SB Resource , is achieved by supporting the TR&D projects, the DBP portfolio, and the Community Engagement mission. The proposed nMS  SB Resource will develop a native mass spectrometry characterization workflow that will quickly provide structural information on protein complexes that are not amenable to, or not yet at a stage appropriate for, other complementary high- resolution structural biology tools. The data (m/z, stoichiometry, heterogeneity, connectivity, topology, and relative subunit binding strengths of protein complexes) will be acquired relatively quickly, on samples that are not highly purified, and on smaller sample quantities than required for most traditional tools. A broad user community will be trained and results disseminated by presentations, publications, and device installations, with a long-term goal of technology commercialization (integrated surface-induced dissociation with high resolution ion mobility and UV photodissociation). Meeting these combined goals requires effective administration and management as described in the three aims below.  Aim 1. Define an organizational structure and staff responsibilities that provide scientific leadership, direction, and coordination to foster communication and collaboration among the Resource components.  Aim 2. Define resource operating procedures, working with the external and internal advisory committees to ensure sound fiscal management and efficient administrative support to all Resource components.  Aim 3. Assess the effectiveness of the Resource and its progress toward sustainability. Dissemination efforts through training, short courses, workshops, and publications, installations of devices, and vendor commitments to support beta installations and commercialize, are key measures of sustainability.

项目摘要：行政和管理。原住民弥撒的建议资源 光谱引导结构生物学（）汇集了一个优秀的科学团队， nMS SMB资源 领导人谁，与选定的工作人员，博士后研究人员和研究生的支持，是装备精良 共同推进拟议的科学、传播和培训。这个团队将共同努力， 为蛋白质：蛋白质、膜蛋白：脂质的结构表征提供集成的工作流程，以及 RNA：蛋白质复合物。拟议的表征工作流程将有助于更好地了解 蛋白质复合物执行其正常的生物学功能，结构的改变如何导致疾病， 如何构建合成模拟物以实现所需的生物医学应用。通过整合所有组件 的 nMS SMB资源 (TR及部门、残疾人士计划及社区参与），我们会引导 开发了本地MS工作流程，从专家及其合作者使用的吸引人的技术到 广泛的前沿工具，可用于指导其他生物化学和结构工具。 行政和管理核心的总体目标是确保资源中心的愿景 对于原生质谱引导的结构生物学， nMS SMB资源 ，是通过支持 TR&D项目、DBP组合和社区参与使命。拟议 nMS SMB资源 将开发一个本地质谱表征工作流程，快速提供结构信息 蛋白质复合物，不服从，或尚未在一个阶段适合，其他互补的高， 解析结构生物学工具。数据（m/z、化学计量、异质性、连接性、拓扑结构和 蛋白质复合物的相对亚基结合强度）将相对快速地获得， 不是高度纯化的，并且比大多数传统工具所需的样品量更少。广泛的用户 将对社区进行培训，并通过演示、出版物和设备安装来传播结果， 技术商业化的长期目标（高分辨率集成表面诱导解离 离子迁移率和UV光解离）。实现这些综合目标需要有效的行政管理， 下面三个目标中所述的管理。 1.确定提供科学领导的组织结构和工作人员职责， 指导和协调，以促进资源组成部分之间的沟通和协作。 第二章.与外部和内部咨询委员会合作，定义资源操作程序 确保健全的财政管理和向所有资源部门提供有效的行政支助。 第三章.评估资源的有效性及其在可持续性方面的进展。传播 通过培训、短期课程、讲习班和出版物、设备安装和供应商 支持beta安装和商业化的承诺是可持续性的关键措施。