Administration and Management of the Resource for Native MS Guided Structural Biology

项目摘要：行政与管理。原住民群众的建议资源 光谱引导结构生物学()汇聚了一支杰出的科学团队 网管SB资源 在精选的工作人员、博士后研究人员和研究生的支持下，装备精良的领导者 协力推进拟议的科学、传播和培训。这个团队将共同努力， 为蛋白质：蛋白质、膜蛋白质：脂类和 RNA：蛋白质复合体。建议的角色化工作流程将有助于更好地了解 蛋白质复合体执行其正常的生物功能，结构改变如何导致疾病，以及 如何构建合成模拟物以实现所需的生物医学应用。通过集成所有组件 的 网管SB资源 (R&D、DBP和社区参与)，我们将引导 开发了原生MS工作流，从专家及其合作者使用的吸引人的技术到 广泛的前线工具，可用于指导其他生化和结构工具。 行政和管理核心的总体目标是确保资源的愿景 对于天然质谱学指导的结构生物学， 网管SB资源 ，是通过支持 R&D项目、DBP投资组合和社区参与使命。建议数 网管SB资源 将开发一种本地的质谱学表征工作流程，该工作流程将快速提供结构信息 在蛋白质复合体上，这些蛋白质复合体不能或尚不处于适合于其他互补高- 解析结构生物学工具。数据(m/z、化学计量、异质性、连通性、拓扑和 蛋白质复合体的相对亚基结合强度)将相对较快地获得 纯度不高，而且样本量比大多数传统工具所需的样本量少。广泛的用户 将通过演示、出版物和设备安装对社区进行培训并传播结果， 技术商业化的长期目标(高分辨率集成表面诱导解离 离子迁移率和紫外光解离)。实现这些综合目标需要有效的管理和 管理，如以下三个目标所述。 目标1.确定提供科学领导的组织结构和员工职责， 指导和协调，以促进资源组成部分之间的沟通和合作。 目标2.定义资源运作程序，与外部和内部咨询委员会合作 确保对所有资源部门进行健全的财政管理和有效的行政支持。 目标3.评估资源的有效性及其在实现可持续性方面的进展。传播 通过培训、短期课程、研讨会和出版物、设备安装和供应商进行的工作 支持测试版安装和商业化的承诺，是衡量可持续性的关键措施。

Project Summary: Administration and Management. The proposed Resource for Native Mass Spectrometry Guided Structural Biology ( ) brings together a team of outstanding scientific nMSSB Resource leaders who, with the support of select staff, postdoctoral researchers, and graduate students, are well-equipped to collaboratively advance the proposed science, dissemination, and training. This team will work together to produce an integrated workflow for structural characterization of protein:protein, membrane protein:lipid, and RNA:protein complexes. The proposed characterization workflow will lead to a better understanding of how protein complexes carry out their normal biological functions, how alterations in architecture lead to disease, and how to build synthetic mimics to carry out desired biomedical applications. Through integration of all components of the nMSSB Resource (TR&Ds, DBPs, and Community Engagement), we will steer conversion of the developed native MS workflow from an appealing technology used by specialists and their collaborators to a widespread frontline tool that can be used to guide other biochemical and structural tools. The overall goal of the Administration and Management Core is to ensure that the vision of the Resource for Native Mass Spectrometry Guided Structural Biology, nMS  SB Resource , is achieved by supporting the TR&D projects, the DBP portfolio, and the Community Engagement mission. The proposed nMS  SB Resource will develop a native mass spectrometry characterization workflow that will quickly provide structural information on protein complexes that are not amenable to, or not yet at a stage appropriate for, other complementary high- resolution structural biology tools. The data (m/z, stoichiometry, heterogeneity, connectivity, topology, and relative subunit binding strengths of protein complexes) will be acquired relatively quickly, on samples that are not highly purified, and on smaller sample quantities than required for most traditional tools. A broad user community will be trained and results disseminated by presentations, publications, and device installations, with a long-term goal of technology commercialization (integrated surface-induced dissociation with high resolution ion mobility and UV photodissociation). Meeting these combined goals requires effective administration and management as described in the three aims below.  Aim 1. Define an organizational structure and staff responsibilities that provide scientific leadership, direction, and coordination to foster communication and collaboration among the Resource components.  Aim 2. Define resource operating procedures, working with the external and internal advisory committees to ensure sound fiscal management and efficient administrative support to all Resource components.  Aim 3. Assess the effectiveness of the Resource and its progress toward sustainability. Dissemination efforts through training, short courses, workshops, and publications, installations of devices, and vendor commitments to support beta installations and commercialize, are key measures of sustainability.