Wyoming Sensory Biology COBRE
怀俄明州感官生物学 COBRE
基本信息
- 批准号:10398656
- 负责人:
- 金额:$ 25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:Administrative SupplementBiologyCell NucleusCellsCenters of Research ExcellenceCollaborationsContract ServicesCore FacilityDry IceFacilities and Administrative CostsFosteringFoundationsFundingGene ExpressionGene Expression ProfileGenesGenetic TranscriptionGenomicsGrantHeterogeneityIndividualInjuryLettersLibrariesMedialMental DepressionMicroscopyMissionMolecular AnalysisNatural regenerationNervous system structureNeuronsPhasePrefrontal CortexPreparationProtocols documentationPublicationsQuality ControlResearchResearch PersonnelResearch Project GrantsRewardsSamplingSensorySensory DisordersServicesShippingShipsStressSystemTrainingUniversitiesVendorWagesWyomingbasecell typecostexperimental studyinstrumentoperationsensory systemsingle-cell RNA sequencingtranscriptome sequencingtranscriptomics
项目摘要
The objective of this administrative supplement is to enhance our research capabilities in the transcriptomic
characterization of the nervous system, and forge a more synergistic interaction among SBC investigators by
promoting transcriptomic research and collaborations. These funds will be used to acquire a NextSeq550
Sequencing System (Illumina) that will significantly enhance SBC’s access to transcriptomic research, and
strengthen the services provided by the Molecular Analysis Unit (MAU) of the Integrated Microscopy Core
(IMCore). Over the past 4-year funding period, the IMCore has made significant advances towards assisting
SBC project leaders with their research involving transcriptomics. Currently, there have already been single cell
RNAseq and Chipseq experiments that have been conducted in the core, as evidenced in our recent publications.
However, these experiments were only able to be carried out through shipping out samples to multiple third-
party service centers and core facilities (e.g., Genewiz.com 10x genomics and Harvard Bauer Core Facilities).
Although this approach has “sufficed”, several major issues hinder its application among SBC researchers: 1)
the shipped samples require -80 degree Fahrenheit/Celsius storage, and transporting them in dry ice adds
unnecessary variability to the sample qualities; 2) the quality controls of library preparations are highly variable
as they are conducted by different commercial vendors that use different protocols; 3) lack of hands-on technical
support inhibits application by new project leaders. Therefore, the purchase of the NextSeq550 will be a game
changer for transcriptomic research at the University of Wyoming. We anticipate that the number of projects
using single cell RNAseq or the Chipseq approach will increase significantly given the availability of the
NextSeq550. The purchase of NextSeq550 is fully justifiable, reasonable, and allocable to grant projects – we
currently have four new research projects that outline utilizing this instrument. Project 1: to identify state-
dependent changes in neuronal transcription at the level of individual cells. Project 2: single-nucleus RNA-seq
will be used to explore transcriptional heterogeneity in the medial prefrontal cortex with differential projections
during stress-induced depression. Project 3: to investigate gene expression within reward-specific ensembles –
defined as a discrete number of neurons that become synchronously activated – and how that differs in neurons
that become activated during the seeking of different types of rewards. Project 4: to answer the following
questions: (1) which genes are activated/repressed after injury and during regeneration, and (2) are the
transcriptional profiles generated different based on injury mechanism and/or cell type. We anticipate that this
administrative supplement will significantly help increase our access to transcriptomic research and
collaboration, increase the number of projects that would utilize this instrument during the remainder of the
funding period, and help build a strong foundation for Phase II applications.
本行政补充的目的是提高我们在转录组学方面的研究能力。
神经系统的表征,并通过以下方式在SBC研究人员之间建立更具协同作用的互动:
促进转录组学研究和合作。这些资金将用于收购NextSeq550
测序系统(Illumina公司),这将大大提高SBC的获得转录组学研究,
加强综合显微镜中心的分子分析组提供的服务
(IMCore).在过去四年的资助期间,IMCore在协助
SBC项目负责人与他们的研究涉及转录组学。目前,已经有单细胞
RNAseq和Chipseq实验已经在核心中进行,正如我们最近的出版物所证明的那样。
然而,这些实验只能通过将样本运送到多个第三方来进行,
党的服务中心和核心设施(例如,Genewiz.com
虽然这种方法已经“足够”,但有几个主要问题阻碍了SBC研究人员的应用:
运输的样品需要-80华氏度/摄氏度的储存,并且在干冰中运输它们增加了
样品质量的不必要的可变性; 2)文库制备的质量控制是高度可变的
因为它们是由使用不同协议的不同商业供应商进行的; 3)缺乏实际操作技术
新的项目领导人因支助而无法申请。因此,购买NextSeq550将是一个游戏
他是怀俄明州大学转录组学研究的改变者。我们预计,
使用单细胞RNAseq或Chipseq方法将显著增加,
下一个Seq550。购买NextSeq550是完全合理的,合理的,可分配给赠款项目-我们
目前有四个新的研究项目,概述了利用这一工具。项目1:确定国家-
在单个细胞水平上神经元转录的依赖性变化。项目2:单核RNA-seq
将被用来探索转录异质性的内侧前额叶皮层与差异投影
压力导致的抑郁症项目3:研究奖励特定集合中的基因表达-
定义为同步激活的离散数量的神经元-以及神经元之间的差异
在寻求不同类型的奖励时被激活。项目4:回答以下问题
问题:(1)哪些基因在损伤后和再生过程中被激活/抑制,以及(2)哪些基因在损伤后和再生过程中被激活/抑制。
基于损伤机制和/或细胞类型产生不同的转录谱。我们预计,
行政补充将大大有助于增加我们获得转录组学研究,
通过合作,增加在本报告所述期间利用这一工具的项目数量,
资助期间,并帮助建立第二阶段应用程序的坚实基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Qian-Quan Sun其他文献
Qian-Quan Sun的其他文献
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{{ truncateString('Qian-Quan Sun', 18)}}的其他基金
A Long-range Recurrent Neural Network Mediates Threat Induced Innate Sensorimotor Integrations
远程循环神经网络介导威胁诱发的先天感觉运动整合
- 批准号:
10539071 - 财政年份:2022
- 资助金额:
$ 25万 - 项目类别:
A Long-range Recurrent Neural Network Mediates Threat Induced Innate Sensorimotor Integrations
远程循环神经网络介导威胁诱发的先天感觉运动整合
- 批准号:
10626968 - 财政年份:2022
- 资助金额:
$ 25万 - 项目类别:
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