Biological and social mediators of child wellbeing among ethnic groups in Fragile Families

项目摘要 自2000年以来，脆弱家庭和儿童福祉研究(FFCWS)为社会科学界提供了 有近5000名出生在大城市的美国儿童的纵向出生队列数据。FFCWS包含一个 大量种族和少数民族：47%的黑人儿童，27%的西班牙裔儿童(17%的儿童出生于 移民西班牙裔父母)。大约40%的家庭生活在贫困线以下。这些功能使 这些数据在其他大规模的纵向研究中是独一无二的，因此非常适合研究健康和 弱势群体的福祉。 在目前的奖项(R01HD076592)下，我们的团队开发了大量的遗传学、表观遗传学和 9岁和15岁儿童的端粒长度(TL)数据。虽然最初的资金是用来制作一份关于 在72个基因中的197个SNP变体(在受试者的子集中)，我们已经能够利用价格降低和 其他资金，为大约3000名儿童提供基因分型。这些数据将提供给 今年，所有9岁和15岁儿童的TL数据也将进入研究界。DNA甲基化 工作已经从最初的500个儿童样本扩大到2200个配对样本(9岁和15岁) 样本。我们计划在2020年测量完成后提供这些数据。 延续了FFCWS从一开始就标志着FFCWS的服务传统，在这次更新的第一年 项目，我们将：目标1，制定一系列相对较新的措施，包括：a)多基因得分 (PG)，包括几个利用基因表达或实验数据来增强预测能力的方法 特定的表型，b)注解到基因的CNV，c)儿童的表观遗传结构，例如甲基化年龄， 甲基化数量性状基因座(MeQTL)和表观基因组范围关联研究(EWAS)研究综述 分数，基于基因和DNA甲基化数据。所有这些衍生措施都将完成并 在获奖的第一年公开可用。这些统计数据将为基因研究提供重要的新工具 对少数民族人口的评估。目标2，对FFCWS母亲进行多种族基因分型， 允许研究父母通过遗传(包括 遗传修养)和社会途径。由于最近呼吁扩大基因组-这项工作具有更大的价值- 非欧洲血统(特别是非洲和西班牙裔)个人和儿童的广泛工作。在《目标3》中， 我们探索了大规模TL研究中有关DNA收集、处理和存储的最佳实践，包括 唾液、新鲜血液和储存的新生儿干血斑点(NDBS)。这些实验将有助于推动达成共识 与美国人口协会举行的生物标记网络会议有关的会议。

Project Summary Since 2000, the Fragile Families and Child Wellbeing Study (FFCWS) has provided the social science community with data on a longitudinal birth cohort of nearly 5000 American children born in large cities. FFCWS contains a large number of racial and ethnic minorities: 47% Black children, 27% Hispanic children (17% children born to immigrant Hispanic parents). Approximately 40% of the families live below the poverty line. These features make the data unique among other large-scale, longitudinal studies, and thus well-suited for studying the health and wellbeing of vulnerable populations. Under the current award (R01HD076592), our team has developed a large portfolio of genetic, epigenetic and telomere length (TL) data for children at ages 9 and 15 years. While originally funded to produce an analysis of 197 SNP variants in 72 genes (in a subset of the subjects), we have been able to leverage price reductions and other funding to provide genotypes on approximately 3000 children. These data will be made available to the research community this year, as will TL data for all children at 9 and 15 years of age. The DNA methylation effort has expanded to 2200 paired samples of children (at 9 and 15 years) from the originally funded 500 samples. We plan to make these data available in 2020, when measurement is complete. Continuing the tradition of service that has marked FFCWS from the beginning, in the first year of this renewal project, we will: Aim 1, develop a portfolio of relatively new measures to include: a) multiple polygenetic scores (PGS), including several that exploit gene expression or experimental data to enhance the predictive power for particular phenotypes, b) CNV annotated to gene, c) epigenetic constructs for children, such as methylation age, methylation quantitative trait loci (meQTL) and epigenome-wide association study (EWAS)-derived summary scores, based on genetic and DNA methylation data. All of these derived measures would be completed and made publicly available by year 1 of an award. These statistics will provide important new tools for the genetic assessment of ethnic minority populations. Aim 2, genotype the FFCWS mothers with a multi-ethnic array, allowing a study of parental effects on child health, behavior, and wellbeing through both genetic (including genetic nurturance) and social pathways. This work takes on greater value due to recent calls to expand genome- wide work for non-European ancestry (in particular African and Hispanic) individuals and in children. In Aim 3, we explore best practices around collection, processing and storage of DNA for large scale TL research involving saliva, fresh blood, and stored neonatal dried blood spots (nDBS). These experiments will help fuel a consensus conference associated with the Biomarker Network meeting held with the Population Association of America.