Biological and social mediators of child wellbeing among ethnic groups in Fragile Families

项目摘要 自2000年以来，脆弱家庭和儿童福利研究（FFCWS）为社会科学界提供了 这是一个纵向出生队列的数据，包括近5000名出生在大城市的美国儿童。FFCWS包含 大量的种族和少数民族：47%的黑人儿童，27%的西班牙裔儿童（17%的儿童出生于 移民西班牙裔父母）。大约40%的家庭生活在贫困线以下。这些特性使得 这些数据在其他大规模纵向研究中是独一无二的，因此非常适合研究健康和 弱势群体的福祉。 根据目前的奖项（R 01 HD 076592），我们的团队已经开发了一个大的遗传，表观遗传和 9岁和15岁儿童的端粒长度（TL）数据。虽然最初的供资是为了分析 72个基因中的197个SNP变异（在受试者的一个子集中），我们已经能够利用降价， 其他资金用于为大约3000名儿童提供基因型。这些数据将提供给 今年，研究界将公布所有9岁和15岁儿童的TL数据。DNA甲基化 这项工作已经从最初资助的500个儿童配对样本（9岁和15岁）扩大到2200个 样品我们计划在2020年完成测量后提供这些数据。 在这次更新的第一年，继续FFCWS从一开始就标志着的服务传统 项目，我们将：目标1，开发一个相对较新的措施组合，包括：a）多个多基因得分 (PGS)，包括几个利用基因表达或实验数据来提高预测能力的方法。 特定表型，B）注释到基因的CNV，c）儿童的表观遗传构建体，例如甲基化年龄， 甲基化数量性状基因座（meQTL）和表观基因组全关联研究（EWAS）的总结 评分，基于遗传和DNA甲基化数据。所有这些衍生措施都将完成， 在颁奖的第一年公布。这些统计数据将为遗传学提供重要的新工具。 评估少数民族人口。目标2，用多种族阵列对FFCWS母亲进行基因分型， 允许研究父母对儿童健康，行为和幸福的影响，通过遗传（包括 遗传养成）和社会途径。由于最近呼吁扩大基因组，这项工作具有更大的价值- 在非欧洲血统（特别是非洲和西班牙裔）的个体和儿童中广泛开展工作。在目标3中， 我们探索了大规模TL研究中DNA收集、处理和储存的最佳实践， 唾液、新鲜血液和储存的新生儿干血斑（nDBS）。这些实验将有助于达成共识 与美国人口协会举行的生物标志物网络会议有关的会议。

Project Summary Since 2000, the Fragile Families and Child Wellbeing Study (FFCWS) has provided the social science community with data on a longitudinal birth cohort of nearly 5000 American children born in large cities. FFCWS contains a large number of racial and ethnic minorities: 47% Black children, 27% Hispanic children (17% children born to immigrant Hispanic parents). Approximately 40% of the families live below the poverty line. These features make the data unique among other large-scale, longitudinal studies, and thus well-suited for studying the health and wellbeing of vulnerable populations. Under the current award (R01HD076592), our team has developed a large portfolio of genetic, epigenetic and telomere length (TL) data for children at ages 9 and 15 years. While originally funded to produce an analysis of 197 SNP variants in 72 genes (in a subset of the subjects), we have been able to leverage price reductions and other funding to provide genotypes on approximately 3000 children. These data will be made available to the research community this year, as will TL data for all children at 9 and 15 years of age. The DNA methylation effort has expanded to 2200 paired samples of children (at 9 and 15 years) from the originally funded 500 samples. We plan to make these data available in 2020, when measurement is complete. Continuing the tradition of service that has marked FFCWS from the beginning, in the first year of this renewal project, we will: Aim 1, develop a portfolio of relatively new measures to include: a) multiple polygenetic scores (PGS), including several that exploit gene expression or experimental data to enhance the predictive power for particular phenotypes, b) CNV annotated to gene, c) epigenetic constructs for children, such as methylation age, methylation quantitative trait loci (meQTL) and epigenome-wide association study (EWAS)-derived summary scores, based on genetic and DNA methylation data. All of these derived measures would be completed and made publicly available by year 1 of an award. These statistics will provide important new tools for the genetic assessment of ethnic minority populations. Aim 2, genotype the FFCWS mothers with a multi-ethnic array, allowing a study of parental effects on child health, behavior, and wellbeing through both genetic (including genetic nurturance) and social pathways. This work takes on greater value due to recent calls to expand genome- wide work for non-European ancestry (in particular African and Hispanic) individuals and in children. In Aim 3, we explore best practices around collection, processing and storage of DNA for large scale TL research involving saliva, fresh blood, and stored neonatal dried blood spots (nDBS). These experiments will help fuel a consensus conference associated with the Biomarker Network meeting held with the Population Association of America.