Shared Resource - Proteomics & Metabolomics Shared Resource

摘要 疾病过程的分子特征已成为当前癌症不可或缺的组成部分 研究。蛋白质组学与代谢组学共享资源(PMSR)的使命是提供最新的 蛋白质组学和代谢组学应用的ART色谱学和质谱学服务。PMSR 2006年为蛋白质组学而成立，并于2008年扩展到代谢组学。自上次审查以来，PMSR的 活动和吞吐量大幅增加；PMSR为22个乔治敦隆巴迪提供了服务 2017财年(FY)综合癌症中心(LCCC)成员。拉多斯拉夫·戈德曼博士，监督 蛋白质组学运营到2018年3月，当时他卸任，专注于指导他新成立的 糖科学中心。拜尔斯接替了高盛。拜尔斯是PMSR的创始人和前主任。 蛋白质组学服务包括优化工作流程和开发专门服务，其中包括识别 蛋白质和多肽及其修饰和定量。靶向高效液相色谱-质谱法 特定蛋白质及其修饰的LC-MS实验有助于进行 翻译研究应用程序。Cheema管理新陈代谢组学部分。代谢组学 组件包括来自各种基质的全面代谢组学和脂类组学分析服务， 包括组织和培养的细胞以及生物体液，如血清、血浆和尿液。PMSR已经开发出 以及从细胞、粪便、导管等复杂基质中提取蛋白质和代谢物的优化方法 灌洗液和脑脊液。蛋白质组学和代谢组学服务还包括多种反应 基于监测(MRM)的靶向量化和代谢组学服务进一步包括高分辨率MS 对于小分子来说。沃特斯公司认可代谢组学部分为创新中心。 沃特斯不断支持高影响力的科学，导致了多笔外部资助的赠款 代谢组学关注至少一个特定的目标。

ABSTRACT The molecular characterization of disease processes has become an indispensable component of current cancer research. The mission of the Proteomics & Metabolomics Shared Resource (PMSR) is to provide state-of-the- art chromatographic and mass spectrometric services for proteomics and metabolomics applications. The PMSR was established in 2006 for proteomics and expanded to metabolomics in 2008. Since the last review, PMSR’s activity and throughput have expanded dramatically; the PMSR provided services to 22 Georgetown Lombardi Comprehensive Cancer Center (LCCC) members in fiscal year (FY) 2017. Radoslav Goldman, PhD, oversaw proteomics operations through 03/2018, at which time he stepped down to focus on directing his newly formed Glycoscience Center. Byers replaced Goldman. Byers is the founder and a former director of the PMSR. Proteomics services include optimizing workflows and developing specialized services, which include identifying proteins and peptides and their modifications and quantification. Targeted liquid chromatography-mass spectrometry (LC-MS) experiments for specific proteins and their modifications facilitate the conduct of translational research applications. Cheema manages the metabolomics component. The metabolomics component includes comprehensive metabolomics and lipidomics profiling services from a variety of matrices, including tissue and cultured cells and biofluids, such as serum, plasma and urine. The PMSR has developed and optimized methods for protein and metabolite extraction from complex matrices such as cells, feces, ductal lavage fluid and cerebrospinal fluid. The proteomics and metabolomics services also include multiple reaction monitoring (MRM)-based targeted quantification, and metabolomics services further include high-resolution MS for small molecules. The Waters Corporation recognized the metabolomics component as a Center of Innovation. Waters has continually supported high-impact science, resulting in multiple extramurally funded grants with a metabolomics focus in at least one specific aim.