Shared Resource - Proteomics & Metabolomics Shared Resource

共享资源 - 蛋白质组学

• 批准号: 10688611
• 负责人: Junfeng Ma
• 金额: $ 24.96万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10688611
• 关键词: Academic Medical Centers; Basic Science; Bioinformatics; Biological Assay; Biology; Cancer Biology; Cancer Center Support Grant; Cells; Cerebrospinal Fluid; Clinical; Clinical Research; Clinical Trials; Collaborations; Complex; Comprehensive Cancer Center; Cultured Cells; DNA Adducts; Data Analyses; Development; Disease; Doctor of Philosophy; Duct (organ) structure; Extramural Activities; Feces; Funding; Goals; Grant; Infrastructure; Investigational Therapies; Irrigation; Knowledge; Laboratories; Liquid substance; Malignant Neoplasms; Methods; Mission; Modification; Molecular; New Jersey; Peptides; Plasma; Primary carcinoma of the liver cells; Principal Investigator; Process; Proteins; Proteomics; Proteomics Shared Resource; Publications; RNA Splicing; Research; Research Personnel; Resolution; Resource Sharing; Sampling; Science; Scientist; Serum; Services; Site; Technology; Time; Tissues; Training; Translational Research; United States National Institutes of Health; Universities; Urine; Variant; Washington; Water; anticancer research; arm; base; data acquisition; data reduction; exosome; experience; experimental study; glycoproteomics; industry partner; innovation; lipidomics; liquid chromatography mass spectrometry; malignant breast neoplasm; member; metabolome; metabolomics; method development; molecular oncology; multiple reaction monitoring; novel; operation; phosphoproteomics; programs; protein metabolite; small molecule; tool

ABSTRACT The molecular characterization of disease processes has become an indispensable component of current cancer research. The mission of the Proteomics & Metabolomics Shared Resource (PMSR) is to provide state-of-the- art chromatographic and mass spectrometric services for proteomics and metabolomics applications. The PMSR was established in 2006 for proteomics and expanded to metabolomics in 2008. Since the last review, PMSR’s activity and throughput have expanded dramatically; the PMSR provided services to 22 Georgetown Lombardi Comprehensive Cancer Center (LCCC) members in fiscal year (FY) 2017. Radoslav Goldman, PhD, oversaw proteomics operations through 03/2018, at which time he stepped down to focus on directing his newly formed Glycoscience Center. Byers replaced Goldman. Byers is the founder and a former director of the PMSR. Proteomics services include optimizing workflows and developing specialized services, which include identifying proteins and peptides and their modifications and quantification. Targeted liquid chromatography-mass spectrometry (LC-MS) experiments for specific proteins and their modifications facilitate the conduct of translational research applications. Cheema manages the metabolomics component. The metabolomics component includes comprehensive metabolomics and lipidomics profiling services from a variety of matrices, including tissue and cultured cells and biofluids, such as serum, plasma and urine. The PMSR has developed and optimized methods for protein and metabolite extraction from complex matrices such as cells, feces, ductal lavage fluid and cerebrospinal fluid. The proteomics and metabolomics services also include multiple reaction monitoring (MRM)-based targeted quantification, and metabolomics services further include high-resolution MS for small molecules. The Waters Corporation recognized the metabolomics component as a Center of Innovation. Waters has continually supported high-impact science, resulting in multiple extramurally funded grants with a metabolomics focus in at least one specific aim.

摘要 疾病过程的分子表征已成为当前癌症不可或缺的组成部分 research.蛋白质组学和代谢组学共享资源（PMSR）的使命是提供 用于蛋白质组学和代谢组学应用的色谱和质谱服务。PMSR 成立于2006年的蛋白质组学，并于2008年扩展到代谢组学。自上次审查以来，PMSR的 活动和吞吐量急剧增加; PMSR为22个乔治敦隆巴迪提供服务 2017财年（FY）综合癌症中心（LCCC）成员。Radoslav Goldman，博士，监督 2018年3月，他卸任，专注于指导他新成立的 糖科学中心。拜尔斯取代了高盛。拜尔斯是PMSR的创始人和前董事。 蛋白质组学服务包括优化工作流程和开发专业服务，其中包括识别 蛋白质和肽及其修饰和定量。靶向液相色谱-质谱联用 特定蛋白质及其修饰的LC-MS实验有助于进行 翻译研究应用。Cheema管理代谢组学组件。代谢组学 组件包括来自各种基质的综合代谢组学和脂质组学分析服务， 包括组织和培养的细胞以及生物流体，例如血清、血浆和尿。PMSR开发了 以及从细胞、粪便、导管等复杂基质中提取蛋白质和代谢物的优化方法 灌洗液和脑脊液。蛋白质组学和代谢组学服务还包括多反应 基于MRM的靶向定量和代谢组学服务还包括高分辨率MS 对于小分子来说。沃茨公司将代谢组学组件视为创新中心。 沃茨一直支持高影响力的科学，导致多个校外资助的赠款， 代谢组学集中在至少一个特定的目标。