Animal Resources

摘要-动物资源共享资源 动物资源共享资源是由癌症中心管理的共享资源，提供 为中心研究人员提供全面的动物护理和使用计划。该设施已获得AAALAC认证 从1988年开始。该中心提供五种基本服务：(1)畜牧和设备卫生/消毒， (2)小鼠繁育程序和维护；(3)程序技术培训和支持；(4)活体内 成像、血液分析和辐射；以及(5)兽医服务和行政/IACUC支持。这个 动物设施目前占地25,602平方米。四栋建筑的空间大小。老鼠是主要的物种，有 偶尔会有老鼠的住所。在过去5年的资助期内，AR平均每只占用8,389个鼠笼 一个月，近两年增长到每月9,000多个网箱。这些老鼠被单独安置在家中 通风的笼子系统。有足够的增长能力，因为该设施能够维持高达 13,000个笼子，但通常以~70%的容量运行，这有利于繁殖群体的维护和 礼宾住房要求。AR提供全方位的动物护理服务，包括所有的饲养和安置 饲养、断奶、获取尾巴样本、维持动物谱系、普查和身份证。这 综合服务也具有成本效益，每日费用包括以下服务：每笼0.72美元 标准小鼠。AR工作人员为中心调查人员提供其他一系列服务，包括 静脉(尾静脉和眶后静脉)、皮下、皮内、肌肉内、腹膜内和 乳房脂肪垫注射，口腔灌胃，肿瘤测量，血液和组织采集，体重， 输液，以及手术前后的护理。核心为中心提供必要的科学支持 通过为肿瘤发展研究提供动物模型和专家技术援助 并在体内生长。肿瘤分析设施是这方面的关键资源，提供访问各种 异种移植模型，包括患者来源的异种移植，并进一步协助实验设计和 对影响肿瘤生长和转移的遗传因素或实验化合物进行研究。 这项服务由一系列用于评估肿瘤进展的复杂成像方式支持， 包括发光、荧光和超声波。其他动物分析服务包括血细胞， 血清、行为和代谢分析。在过去的五年里，来自三个癌症中心的44名研究人员 项目广泛利用了动物资源，核心为 癌症中心研究人员发表了116篇与癌症相关的论文。

ABSTRACT – ANIMAL RESOURCES SHARED RESOURCE The Animal Resources Shared Resource is a Cancer Center-managed Shared Resource that provides a comprehensive animal care and use program for Center investigators. The facility has been AAALAC-accredited since 1988. The Core provides five basic types of services: (1) husbandry and equipment sanitation/sterilization, (2) mouse breeding procedures and maintenance; (3) procedural techniques training and support; (4) in vivo imaging, blood analysis, and irradiation; and (5) veterinary services and administrative/IACUC support. The animal facility currently occupies 25,602 sq. ft of space in four buildings. Mice are the primary species used, with occasional housing of rats. Over the last 5-year grant period, AR averaged 8,389 occupied mouse cages per month, with growth in the last 2 years to more than 9,000 cages/month. The mice are housed in individually ventilated caging systems. There is sufficient capacity for growth, as the facility is capable of maintaining up to 13,000 cages but generally operates at ~70% capacity, which facilitates breeding colony maintenance and protocol housing requirements. AR provides full-service animal care that includes all husbandry as well as setting up breedings, weaning litters, obtaining tail samples, maintaining animal pedigrees, census and IDs. This comprehensive service is also cost-effective, with the per diem including those services at $0.72/cage for standard mice. The AR staff perform an additional range of services for Center investigators, including intravenous (tail and retro-orbital veins), subcutaneous, intradermal, intramuscular, intraperitoneal, and mammary fat pad injections, oral gavage, tumor measurements, blood and tissue collection, body weights, perfusions, and pre- and post-operative surgical care. The Core provides essential scientific support to Center members by supplying animal models and expert technical assistance for investigation of tumor development and growth in vivo. The Tumor Analysis facility is a key resource in this respect, providing access to a variety of xenograft models, including patient-derived xenografts, and further assisting with the experimental design and carrying out studies of genetic factors or experimental compounds that influence tumor growth and metastasis. This service is supported by an array of sophisticated imaging modalities for evaluating tumor progression, including luminescence, fluorescence, and ultrasound. Additional Animal Analysis services include blood cell, serum, behavioral, and metabolic analysis. In the past five years, 44 Investigators from all three Cancer Center programs have made extensive use of the Animal Resources, and the Core has provided support for the publication of 116 cancer-relevant papers by Cancer Center investigators.