权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Core B: Mouse Models of Aging and Cancer

核心 B：衰老和癌症的小鼠模型

基本信息

批准号：
10270684
负责人：
Alessandra Sacco
金额：
$ 31.13万
依托单位：
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10270684
关键词：
3-Dimensional Age Aging Animal Model Animals Bile Acids Body Weight Cell Aging Cell Transplantation Chromatin Clinical Collaborations Collection DNA DNA delivery Data Databases Development Diet Disease Down-Regulation Early Diagnosis Eating Ensure Environment Equipment Gene Expression Genetic Genotype Goals Harvest Health Hepatocyte Histologic Histopathology Human Resources Immunomodulators Incidence Individual Industry Standard Inflammation Infrastructure Injections Interferons Intervention Intravenous Investigation Lead Liver Maintenance Malignant Neoplasms Malignant neoplasm of liver Measurement Metabolism Microbiology Mitochondria Mitochondrial DNA Molecular Molecular Analysis Monitor Mus Natural Immunity Oral Outcome Pain Performance Prevention Prevention strategy Procedures Regimen Reporting Reproducibility Reproducibility of Results Research Personnel Research Project Grants Risk Factors Role Sentinel Services Signal Transduction Sirolimus Soil Stress Tail Techniques Testing Tissue Banks Tissues Training Veins Water Work adaptive immunity aged animal imaging dietary epigenome immunoregulation in vivo inhibitor/antagonist interest intraperitoneal liver cancer prevention mouse model non-alcoholic fatty liver disease non-genetic novel therapeutics success tumor tumor growth viral DNA viral detection

项目摘要

PROJECT SUMMARY – CORE B Understanding aging in the liver and the role of aging in liver cancer development will require investigation in live animals as well as genetic and non-genetic interventions that target age dysregulation, for their ability to suppress the onset of liver cancer. The Mouse Models of Aging and Cancer Core (Core B) will achieve this goal by offering the following services: (1) Mouse husbandry, collecting tail-snips for genotyping, and longitudinal monitoring of key parameters (body weight, average food intake, tumor measurements); (2) Animal interventions, which include injections (intravenous (IV), retroorbital or tail vein, hydrodynamic tail vein injection (HDTV), intraperitoneal (IP)), viral and DNA delivery, agents delivery (oral gavage, modified diet or water, or injections), cell transplantation; (3) Efficient use of infrastructure and access to equipment for animal imaging to monitor tumor growth; (4) Animal health monitoring using the standard industry-wide practice of soiled bedding sentinels for quarterly viral detection and annual microbiology and histopathology testing; (5) Collection and storage of tissues for histological and molecular analyses; (6) Histopathology expertise to assess liver tissues for stage of disease; (7) A mouse colony database and a tissue banking database accessible to PPG investigators, in collaboration with Core A; (8) Hands-on training in animal handling and procedural techniques for project staff. Core B will benefit from personnel with long-term expertise in animal work. Performance of procedures by skilled Core staff will enhance the reproducibility and scientific quality of the data, as well as minimizing pain and stress for the animals. Core B will participate in all project planning sessions with the Administrative Core (Core A) and PPG investigators to ensure that Core B is functioning to meet programmatic needs. Core B will be responsible for reporting to the Administrative Core, documenting effort and evaluating Core contributions to individual projects. Core B will ensure a centralized maintenance of all animal models to minimize variables of different environments, and ensure rigor and reproducibility of the results.

项目总结-核心b