Core B: Mouse Models of Aging and Cancer

核心 B：衰老和癌症的小鼠模型

• 批准号: 10698101
• 负责人: Alessandra Sacco
• 金额: $ 31.63万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10698101
• 关键词: 3-Dimensional; Age; Aging; Animal Model; Animals; Beds; Bile Acids; Body Weight; Breeding; Cell Aging; Cell Transplantation; Chromatin; Clinical; Collaborations; Collection; Cytoplasm; DNA; DNA delivery; Data; Databases; Development; Diet; Disease; Down-Regulation; Early Diagnosis; Eating; Ensure; Environment; Equipment; Gene Expression; Genetic; Genotype; Goals; Harvest; Health; Hepatocyte; Histologic; Histopathology; Human Resources; Immunomodulators; Incidence; Individual; Industry Standard; Inflammation; Infrastructure; Injections; Interferons; Intervention; Intravenous; Investigation; Liver; Maintenance; Malignant Neoplasms; Malignant neoplasm of liver; Measurement; Metabolism; Microbiology; Mitochondria; Mitochondrial DNA; Molecular; Molecular Analysis; Monitor; Mus; Natural Immunity; Oral; Outcome; Pain; Performance; Prevention; Prevention strategy; Procedures; Regimen; Reporting; Reproducibility; Reproducibility of Results; Research Personnel; Research Project Grants; Risk Factors; Role; Sentinel; Services; Signal Transduction; Sirolimus; Soil; Stress; Tail; Techniques; Testing; Tissue Banks; Tissues; Training; Veins; Viral; Water; Work; adaptive immunity; aged; animal imaging; candidate validation; dietary; epigenome; immune modulating agents; immunoregulation; in vivo; inhibitor; interest; intraperitoneal; liver cancer prevention; mouse model; non-alcoholic fatty liver disease; non-genetic; novel therapeutics; skills; success; tumor; tumor growth; viral detection

PROJECT SUMMARY – CORE B Understanding aging in the liver and the role of aging in liver cancer development will require investigation in live animals as well as genetic and non-genetic interventions that target age dysregulation, for their ability to suppress the onset of liver cancer. The Mouse Models of Aging and Cancer Core (Core B) will achieve this goal by offering the following services: (1) Mouse husbandry, collecting tail-snips for genotyping, and longitudinal monitoring of key parameters (body weight, average food intake, tumor measurements); (2) Animal interventions, which include injections (intravenous (IV), retroorbital or tail vein, hydrodynamic tail vein injection (HDTV), intraperitoneal (IP)), viral and DNA delivery, agents delivery (oral gavage, modified diet or water, or injections), cell transplantation; (3) Efficient use of infrastructure and access to equipment for animal imaging to monitor tumor growth; (4) Animal health monitoring using the standard industry-wide practice of soiled bedding sentinels for quarterly viral detection and annual microbiology and histopathology testing; (5) Collection and storage of tissues for histological and molecular analyses; (6) Histopathology expertise to assess liver tissues for stage of disease; (7) A mouse colony database and a tissue banking database accessible to PPG investigators, in collaboration with Core A; (8) Hands-on training in animal handling and procedural techniques for project staff. Core B will benefit from personnel with long-term expertise in animal work. Performance of procedures by skilled Core staff will enhance the reproducibility and scientific quality of the data, as well as minimizing pain and stress for the animals. Core B will participate in all project planning sessions with the Administrative Core (Core A) and PPG investigators to ensure that Core B is functioning to meet programmatic needs. Core B will be responsible for reporting to the Administrative Core, documenting effort and evaluating Core contributions to individual projects. Core B will ensure a centralized maintenance of all animal models to minimize variables of different environments, and ensure rigor and reproducibility of the results.

项目概要-核心B 了解肝脏的衰老以及衰老在肝癌发展中的作用需要在肝脏中进行研究。 动物以及针对年龄失调的遗传和非遗传干预措施，因为它们能够抑制 肝癌的发病衰老和癌症核心小鼠模型（核心B）将通过以下方式实现这一目标： 提供以下服务：（1）小鼠饲养，收集用于基因分型的尾剪， 监测关键参数（体重、平均食物摄入量、肿瘤测量）;（2）动物干预， 其包括注射（静脉内（IV），眶后或尾静脉，流体动力学尾静脉注射（HDTV）， 腹膜内（IP））、病毒和DNA递送、试剂递送（口服管饲、改良的饮食或水、或注射）， 细胞移植;（3）有效利用基础设施和获得动物成像设备以监测 肿瘤生长;（4）使用污染垫料哨兵的标准行业做法监测动物健康 进行每季度的病毒检测和每年的微生物和组织病理学检测;（5）收集和储存 组织学和分子分析;（6）组织学专业知识，以评估肝组织的阶段， （7）PPG研究者可访问的小鼠集落数据库和组织库数据库， 与核心A合作;（8）为项目工作人员提供动物处理和程序技术方面的实践培训。 核心B将受益于具有长期动物工作专业知识的人员。由熟练的 核心工作人员将提高数据的可重复性和科学质量，并尽量减少痛苦和压力 为了动物们核心B将与行政核心（核心A）一起参加所有项目规划会议， 项目规划补助金调查员确保核心B的运作满足方案需要。核心B将负责 向行政核心报告，记录工作并评估核心对个人的贡献 项目核心B将确保所有动物模型的集中维护，以最大限度地减少不同的变量 环境，并确保结果的严谨性和可重复性。