Olfactory neuromodulation of visual circuits and behavior

视觉回路和行为的嗅觉神经调节

• 批准号: 10405378
• 负责人: Mark Arthur Frye
• 金额: $ 6.34万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10405378
• 关键词: Affect; Aging; Behavior; Biogenic Amines; Biological Models; Brain; Cells; Chemicals; Cognitive; Complex; Couples; Degenerative Disorder; Detection; Disease; Drosophila genus; Functional disorder; Genetic Models; Genetic Techniques; Human; Image; Individual; Lead; Logic; Modality; Molecular; Nervous system structure; Neurons; Norepinephrine; Output; Pathology; Pathway interactions; Perception; Population; Process; Property; Research; Sensory; Signal Transduction; Stimulus; Stroke; Synapses; Traumatic injury; United States; Visual; Writing; brain health; chemical function; cognitive function; disability; fly; functional disability; multimodality; multisensory; neuronal circuitry; neuroregulation; nonhuman primate; optogenetics; public health relevance; relating to nervous system; response; two-photon; virtual reality system; visual processing

Project summary A significant percentage of people in the US suffer from disabilities resulting from traumatic injury, stroke, or degenerative disease which result in enigmatic deficits in cognitive function. Therefore, an understanding of the fundamental properties of neuronal circuits for complex brain function, and how these circuits are modulated by biogenic amines, will sharpen our understanding of the normal brain, thereby highlighting pathology and facilitating treatments. A major function of the brain is to integrate information across sensory modalities to enable sharp and robust perception. The cell-circuit mechanism for how different sensory modalities interact is not well understood. This project will capitalize on the significant experimental advantages of the fruit fly Drosophila to explore the molecular logic and neural connections that produce an elementary form of multisensory integration perception. The fly displays robust multisensory perception, integrating olfactory signals with visual processing to enhance perceptual abilities. Furthermore, sensory circuits have been shown to be under robust neuromodulatory control by biogenic amines. Similar processes have been localized to sub- cortical and cortical pathways in humans and non-human primates. The fly has a numerically compact nervous system, with which highly advanced genetic techniques can be used to identify, manipulate, and record activity from individual neurons and neurosecretory cells, as well as their upstream and downstream synaptic partners and molecular components. The PI hypothesizes that the fly brain couples olfactory sensory detection to visual processing through neuromodulatory cells that carry the chemical equivalent of norepinephrine. The PI will perform two-photon Ca2+ imaging to ‘read’ activity from live flies in response to stimuli the PI has discovered elicit robust multi-modal integration behavior within a virtual reality system. The PI will use optogenetics imaging to ‘write’ signals into these circuit pathways to assess input-output functions from an intact behaving fly.

项目摘要 在美国，很大一部分人因创伤而遭受残疾 损伤，中风或退化性疾病，导致认知功能的神秘缺陷。 因此，了解复杂大脑神经元电路的基本特性 功能，以及这些电路如何通过生物胺调节，将使我们的锐化 了解正常大脑，从而突出病理学和支持治疗。 大脑的主要功能是整合跨感官方式的信息以启用 敏锐而强大的感知。细胞电路机制如何不同的感觉方式 互动不太了解。该项目将利用重要的实验 果蝇果蝇的优势探索分子逻辑和神经联系 产生基本形式的多感觉整合感知。苍蝇显示强大的 多感官感知，将嗅觉信号与视觉处理相结合以增强 感知能力。此外，已经证明感觉电路在强大 生物胺神经调节控制。类似的过程已被本地化为子 人类和非人类隐私的皮质和皮质途径。苍蝇有数值 紧凑的神经系统，高度先进的遗传技术可用于 从单个神经元和神经分泌细胞中识别，操纵和记录活性，为 以及它们的上游和下游突触伙伴和分子成分。 pi 假设苍蝇脑伴侣通过嗅觉感觉检测到视觉处理 具有去甲肾上腺素的化学物质的神经调节细胞。 PI会执行 两光子Ca2+成像从活蝇中“读取”活性以响应刺激为PI具有 发现在虚拟现实系统中发现了强大的多模式整合行为。 pi 将使用光遗传学成像将信号“写入”这些电路途径来评估输入输出 来自完整的苍蝇的功能。