Olfactory neuromodulation of visual circuits and behavior

视觉回路和行为的嗅觉神经调节

基本信息

批准号：
10611542
负责人：
Mark Arthur Frye
金额：
$ 4.53万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10611542
关键词：
Affect Aging Animals Ballistics Basic Science Behavior Behavioral Biogenic Amines Biological Models Brain Cells Chemicals Cognitive Collaborations Complex Couples Degenerative Disorder Detection Discrimination Disease Drosophila genus Food Functional disorder Ganglia Genetic Models Genetic Techniques Goals Home Human Image Individual Knowledge Lead Link Locomotion Logic Maps Mission Modality Modeling Molecular Motion Motor Movement National Institute of Neurological Disorders and Stroke Nervous System Neurons Norepinephrine Octopamine Odors Olfactory Pathways Optic Lobe Optics Output Pathology Pathway interactions Pattern Perception Persons Population Process Property Reflex action Research Resolution Saccades Sensory Signal Pathway Signal Transduction Smell Perception Smooth Pursuit Source Spatial Distribution Stimulus Stroke Synapses System Testing Transcript Traumatic injury United States Vision Visual Writing behavioral response brain health chemical function cognitive function detector disability falls fly functional disability improved in vivo in vivo calcium imaging multimodality multisensory neural neurogenetics neuronal circuitry neuroregulation nonhuman primate octopamine receptor optogenetics public health relevance receptive field response sensor sensory input sensory integration two-photon virtual reality virtual reality system visual control visual feedback visual processing

项目摘要

Project summary A significant percentage of people in the US suffer from disabilities resulting from traumatic injury, stroke, or degenerative disease which result in enigmatic deficits in cognitive function. Therefore, an understanding of the fundamental properties of neuronal circuits for complex brain function, and how these circuits are modulated by biogenic amines, will sharpen our understanding of the normal brain, thereby highlighting pathology and facilitating treatments. A major function of the brain is to integrate information across sensory modalities to enable sharp and robust perception. The cell-circuit mechanism for how different sensory modalities interact is not well understood. This project will capitalize on the significant experimental advantages of the fruit fly Drosophila to explore the molecular logic and neural connections that produce an elementary form of multisensory integration perception. The fly displays robust multisensory perception, integrating olfactory signals with visual processing to enhance perceptual abilities. Furthermore, sensory circuits have been shown to be under robust neuromodulatory control by biogenic amines. Similar processes have been localized to sub- cortical and cortical pathways in humans and non-human primates. The fly has a numerically compact nervous system, with which highly advanced genetic techniques can be used to identify, manipulate, and record activity from individual neurons and neurosecretory cells, as well as their upstream and downstream synaptic partners and molecular components. The PI hypothesizes that the fly brain couples olfactory sensory detection to visual processing through neuromodulatory cells that carry the chemical equivalent of norepinephrine. The PI will perform two-photon Ca2+ imaging to ‘read’ activity from live flies in response to stimuli the PI has discovered elicit robust multi-modal integration behavior within a virtual reality system. The PI will use optogenetics imaging to ‘write’ signals into these circuit pathways to assess input-output functions from an intact behaving fly.

项目摘要在美国，很大一部分人因创伤而遭受残疾损伤，中风或退化性疾病，导致认知功能的神秘缺陷。因此，了解复杂大脑神经元电路的基本特性功能，以及这些电路如何通过生物胺调节，将使我们的锐化了解正常大脑，从而突出病理学和支持治疗。大脑的主要功能是整合跨感官方式的信息以启用敏锐而强大的感知。细胞电路机制如何不同的感觉方式互动不太了解。该项目将利用重要的实验果蝇果蝇的优势探索分子逻辑和神经联系产生基本形式的多感觉整合感知。苍蝇显示强大的多感官感知，将嗅觉信号与视觉处理相结合以增强感知能力。此外，已经证明感觉电路在强大生物胺神经调节控制。类似的过程已被本地化为子人类和非人类隐私的皮质和皮质途径。苍蝇有数值紧凑的神经系统，高度先进的遗传技术可用于从单个神经元和神经分泌细胞中识别，操纵和记录活性，为以及它们的上游和下游突触伙伴和分子成分。 pi 假设苍蝇脑伴侣通过嗅觉感觉检测到视觉处理具有去甲肾上腺素的化学物质的神经调节细胞。 PI会执行两光子Ca2+成像从活蝇中“读取”活性以响应刺激为PI具有发现在虚拟现实系统中发现了强大的多模式整合行为。 pi 将使用光遗传学成像将信号“写入”这些电路途径来评估输入输出来自完整的苍蝇的功能。