Neurobiology and Genomics Laboratory Core

神经生物学和基因组学实验室核心

• 批准号: 10408801
• 负责人: J John Mann
• 金额: $ 26.24万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-19 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10408801
• 关键词: Amylases; Autopsy; Biological Assay; Blood; Blood specimen; Body Fluids; Brain; Brain-Derived Neurotrophic Factor; C-reactive protein; DNA; DNA Methylation; Data; Data Set; Epigenetic Process; Exposure to; Gene Expression Profiling; Genetic; Genome; Genomics; Human; Hydrocortisone; Image; Immunologic Monitoring; Interleukin-6; Kynurenine; Laboratories; Methylation; Neopterin; Neurobiology; New York; Pharmaceutical Preparations; Preparation; Psychotropic Drugs; Quinolinic Acid; RNA; Resources; Sampling; Ships; TNF gene; Time; Toxicology; Tryptophan; Universities; brain tissue; cytokine; falls; genetic variant; response; salivary assay; suicidal behavior; transcriptome sequencing

SUMMARY – CORE 3 The Neurobiology and Genetics Laboratory Core (NGLC) will carry out specialized assays for multiple projects and thereby insure optimal and comparable reliability across projects. It offers an economy of scale and moves resources as study priorities change. The assays fall into several major domains. Toxicology assays such as ones using brain tissue provide a longer time frame on most recent exposure to psychotropic drugs than body fluids since brain levels tend to be 10-40 times higher. This will be valuable for the postmortem brain studies in Project 1 where drugs may alter the results. The second domain is genetics and epigenetics. Here assays will include gene variants, methylation and gene expression assays. The latter domain is applicable to Projects 1, 2 and 5. The core will assay salivary amylase and cortisol in Project 5 and the responses will also be correlated with findings from Projects 3 and 4. The core will prepare DNA and RNA for assay by the New York Genome Center checking integrity of the DNA and RNA and preparing samples with the correct concentration for assay. Other assays for P5 include BDNF, PUFAs, Tryptophan, Kynurenine, Quinolinic acid, Neopterin, and preparing samples for cytokine assays and assaying cytokines from TSST samples (IL-6, TNF-alpha, C- reactive protein). Since Project 6 is looking at how suicidal behavior may be predicted by the large imaging data sets from Projects 3 and 4, it will also consider the impact of the laboratory data from Project 5. Thus, findings from this core are relevant directly or indirectly to every project

摘要-核心3 神经生物学和遗传学实验室核心（NGLC）将为多个项目进行专门的分析 从而确保跨项目的最佳和可比较的可靠性。它提供了规模经济， 随着研究重点的变化，资源也会发生变化。这些测定分为几个主要领域。毒理学试验，如 使用脑组织的研究提供了最近接触精神药物的时间框架， 因为大脑的水平往往是10-40倍。这对脑的死后研究具有重要意义。 项目1药物可能改变结果。第二个领域是遗传学和表观遗传学。在这里，分析将 包括基因变异、甲基化和基因表达测定。后一个域适用于项目1， 图2和图5所示。核心将在项目5中测定唾液淀粉酶和皮质醇，并且响应也将是 与项目3和项目4的结果相关。核心将准备DNA和RNA，供纽约实验室进行分析。 基因组中心检查DNA和RNA的完整性并制备具有正确浓度的样品 用于测定。用于P5的其他测定包括BDNF、PUFA、色氨酸、犬尿氨酸、喹啉酸、新蝶呤和抗肿瘤药物。 制备用于细胞因子测定的样品并测定来自TSST样品的细胞因子（IL-6，TNF-α，C- 反应蛋白）。由于项目6正在研究自杀行为如何通过大型成像预测， 除了项目3和项目4的实验室数据集之外，还将考虑项目5的实验室数据的影响。因此，在本发明中， 该核心的发现与每个项目直接或间接相关