Genomics

项目摘要 基因组学共享资源（G-SR）是一个国家的最先进的基因组学设施，致力于提供最新的 低成本的基因组研究工具。G-SR的主要重点是促进高影响癌症研究 SCI成员通过提供最先进的遗传和基因组学工具和标准化检测， 使用这些工具。这一目标是通过为SCI成员提供无障碍的，全面的， 在快速周转时间内以高成本效益的方式获得这些基因组学技术， 咨询服务，涵盖从样品制备到计算的基因组学实验的各个方面 数据分析。主要的G-SR技术和服务包括高通量测序、单细胞基因组测序、 分析，空间基因组分析，基于微阵列的分析，数字和实时定量PCR，多重 单分子分析以及这些技术所需的所有样品制备服务。专家指导 还提供了与每种技术的使用贯穿整个实验过程的指导， 实验设计、数据分析、数据存档和出版。斯坦福大学以前的资源 功能基因组学设施（SFGF），遗传测序服务中心（GSSC），蛋白质和核酸 酸设施（PAN）和遗传生物信息学服务中心（GBSC）现已合并， 在John Coller博士的领导下，作为G-SR主任，提供全方位的服务。迈克尔·斯奈德 PhD（PS）是导师。所有程序的成员定期使用G-SR，最重的使用即将到来 癌症生物学、癌症干细胞和癌症免疫治疗项目。G-SR做出了贡献 到46个以癌症为重点的出版物（30个高影响力）。G-SR的年度预算为3，652，748美元，而CCSG 要求183830美元。因此，G-SR利用广泛的机构支持，仅从 CCSG基金。G-SR的主要科学目标是：（1）提供最先进的基因组学 能力，（2）促进新的实验方法和技术的发展，（3）评估 并将尖端基因组学技术应用于癌症研究，以及（4）提供基因组学培训 技术、实验测定和分析方法。G-SR的未来计划包括扩大癌症- 增加一名全职生物信息学家，提供具体的生物信息学支持，扩大高通量 测序和单细胞基因组能力。

PROJECT SUMMARY The Genomics Shared Resource (G-SR) is a state-of-the-art genomics facility dedicated to providing the latest genomic research tools at a low cost. The primary focus of the G-SR is to facilitate high-impact cancer research by SCI members by providing the most advanced genetic and genomic-based tools and standardized assays using these tools. This goal is accomplished by providing SCI members with unencumbered, comprehensive, and cost-effective access to these genomics technologies at rapid turn-around times with high-touch, expert consultation services that cover all aspects of genomics experiments from sample preparation to computational analysis of data. Major G-SR technologies and services include high-throughput sequencing, single-cell genomic analysis, spatial genomic analysis, microarray-based analysis, digital and real-time quantitative PCR, multiplex single-molecule analysis, and all sample preparation services required for these technologies. Expert guidance with the use of each technology is also provided throughout the entire experimental process, including guidance with experimental design, data analysis, data archiving and publication. The previous resources of the Stanford Functional Genomics Facility (SFGF), the Genetics Sequencing Service Center (GSSC), Protein and Nucleic Acid Facility (PAN), and Genetics Bioinformatics Service Center (GBSC) have been now combined under the leadership of John Coller, PhD, as the G-SR Director, to provide the full spectrum of services. Michael Snyder, PhD (PS), is the faculty advisor. Members of all programs regularly use the G-SR, with the heaviest use coming from the Cancer Biology and Cancer Stem Cells and Cancer Immunotherapy programs. The G-SR contributed to 46 cancer-focused publications (30 high-impact). The annual budget of the G-SR is $3,652,748, yet the CCSG request is $183,830. Accordingly, the G-SR leverages extensive institutional support and seeks only 5% from CCSG funds. The G-SR is focused on the following major scientific aims: (1) to provide state-of-the-art genomics capabilities, (2) to facilitate the development of novel experimental methods and technologies, (3) to evaluate and implement cutting-edge genomics technologies for cancer research, and (4) to provide training on genomics technologies, experimental assays, and analysis methods. Future plans of the G-SR include expanding cancer- specific bioinformatics support with the addition of one full-time bioinformatician, expanding high-throughput sequencing and single-cell genomic capabilities.