High Throughput DNA Sequencer Upgrade

描述（由申请人提供）：该提案要求提供资金，将Illumina基因组分析仪IIx（GAIIx）升级为Illumina HiSeq 2500（HiSeq 2500）。HiSeq 2500是最先进的高通量DNA测序仪，每次测序运行能够生成数亿个读数。拟议的仪器将由斯坦福大学功能基因组学设施（SFGF），斯坦福大学医学院的服务中心提供和维护。该设施提供专业知识、支持、资源和服务 为斯坦福大学和其他当地研究机构的NIH研究人员提供利用微阵列技术和高通量测序的基因组学和蛋白质组学研究。由于许多研究人员已经转向高通量测序（HTS）进行功能基因组研究，并将继续这样做，在未来，这是至关重要的，服务中心保持目前最先进的仪器，使开创性的研究所追求的斯坦福大学的研究人员可以继续。Illumina HiSeq 2500的HTS能力需要满足HTS的需求，并使斯坦福大学和其他地方的NIH资助的研究人员能够进一步了解我们的遗传密码如何影响人类健康和疾病。

DESCRIPTION (provided by applicant): This proposal requests funds to upgrade an Illumina Genome Analyzer IIx (GAIIx) to an Illumina HiSeq 2500 (HiSeq 2500). The HiSeq 2500 is a state-of-the-art high-throughput DNA sequencer that has the ability to generate many hundreds of millions of reads per sequencing run. The proposed instrument will be housed and maintained by the Stanford Functional Genomics Facility (SFGF), a service center in the School of Medicine at Stanford University. The facility offers expertise, support, resources, and services for genomics and proteomics based research utilizing microarray technologies and high-throughput sequencing to NIH researchers at Stanford University and other local research institutions. As many investigators have turned to high-throughput sequencing (HTS) for functional genomic studies and will continue to do so in the future, it is critical that the servic center maintain current state-of-the- art instrumentation so that the pioneering research pursued by Stanford researchers can continue. The HTS capabilities of the propose Illumina HiSeq 2500 are required to meet the demands for HTS and give NIH-funded researchers at Stanford University and elsewhere the ability to further understand how our genetic code affects human health and disease.