Shared Tundra screening cryo-EM for New England

该提案要求支持购买最先进的100千伏筛分低温电磁炉 乐器。这台仪器将被安置在加州大学的一个地理中心位置 位于康涅狄格州法明顿的康涅狄格州健康中心(UCHC)，从而确保所有人都可以方便地访问 参与的研究小组。低温EM将被整合到已建立的UCHC EM中 设施，它具有30多年来公认的卓越研究传统。UCHC EM 该设施由一名全职经理和一名助理主任运营，并得到UCHC的支持。AS 典型的共享仪器，该仪器将不仅可用于核心研究 团体，但也向整个新英格兰将从中受益的其他人。这项建议 结合来自3所大学的研究人员，共有18个研究小组组成核心用户 一群人。研究领域包括病毒学、信号转导、免疫生物学、DNA修复等 许多其他的，对广泛的各种疾病具有关键影响。拟议的研究 项目得到了令人振奋的初步数据的良好支持。18个核心研究小组中的许多 在过去的几年中获得了广泛的低温EM经验，并拥有强大的外部资金 公文包。虽然可以使用高性能的低温电磁仪器，但访问 经济实惠、易于使用的筛选低温EM，允许仔细优化样品 结构确定要么根本不存在，要么昂贵，因此对许多人来说是负担不起 研究小组。所要求的Tundra 100千伏冷冻-EM将解决这一限制，并使 快速、经济高效且易于访问的大型、高度复杂的生物研究 系统。此外，它还将增强学生和博士后的培训能力 研究低温EM的科学家。总而言之，所谓的“解决方案革命”凸显了 新型低温电磁场仪器测定临界生物结构的能力 用来回答基本生物学问题的样本。然而，对于所有的结构生物学来说 技术方面，样本优化是获得这些新见解的关键关键步骤。这个 所需的仪器将为整个新世纪的大量研究人员填补这一空白 英国。此外，重大的机构投资将保证长期的成功和 将使关键的生物功能得以确定。我们相信这台低温电磁仪 不仅将为核心参与实验室的研究提供必要的核心 具体来说，但也将为新英格兰以及美国的研究提供长期的影响 一般来说，是社区。

This proposal requests support for the acquisition of a state-of-the-art 100 kV screening cryo-EM instrument. This instrument will be housed in a geographically central location at the University of Connecticut Health Center (UCHC) in Farmington, CT, thereby ensuring facile access for all participating research groups. The cryo-EM will be integrated into the established UCHC EM facility, which has a proven tradition of research excellence for over 30 years. The UCHC EM facility is operated by a full-time manager and an assistant director, supported by UCHC. As typical for shared instrumentation, this instrument will be available not only to the core research groups, but also to others throughout New England that will benefit from it. This proposal combines researchers from 3 universities, with a total of 18 research groups forming the core user group. Research areas include virology, signal-transduction, immunobiology, DNA repair among many others, with key implications for a broad variety of diseases. The proposed research projects are well supported with exciting preliminary data. Many of the 18 core research groups have gained extensive cryo-EM experience over the last years and have a strong external funding portfolio. While access to high powered cryo-EM instruments is available, the access to affordable, easy-to-use screening cryo-EM’s that allow for the careful optimization of samples for structure determination are either non-existent or expensive and thus out of reach for many research groups. The requested Tundra 100 kV cryo-EM will solve this limitation, and enable rapid, cost-effective and easy access for the investigation of large, highly complex biological systems. Furthermore, it will enhance the training capabilities of students and post-doctoral research scientists for cryo-EM. In summary, the so-called ‘resolution-revolution’ has highlighted the capabilities of novel cryo-EM instruments for the structure determination of critical biological samples to answer fundamental biological questions. However, as for all structural biology techniques, sample optimization is the key essential step for gaining these new insights. The requested instrument will fill this gap for a significant number of researchers throughout New England. Further, significant institutional investments will guarantee the long-term success and will allow key biological functions to be determined. We are confident that this cryo-EM instrument will not only provide a necessary nucleus for the research of the core participating laboratories specifically but will also provide a long-lasting impact for New England, as well as the US research community, generally.