Shared Tundra screening cryo-EM for New England

新英格兰共享 Tundra 冷冻电镜筛查

• 批准号: 10413473
• 负责人: Wolfgang Peti
• 金额: $ 145.74万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2024-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413473
• 关键词: Area; Biological; Biological Process; Cell Nucleus; Communities; Connecticut; Cryoelectron Microscopy; DNA Repair; Data; Disease; Ensure; Funding; Geography; Health; Immunobiology; Investigation; Investments; Laboratories; Location; New England; Postdoctoral Fellow; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Scientist; Signal Transduction; Structure; Students; Techniques; Time; Training; Universities; complex biological systems; cost effective; experience; insight; instrument; instrumentation; novel; screening; structural biology; success; virology

This proposal requests support for the acquisition of a state-of-the-art 100 kV screening cryo-EM instrument. This instrument will be housed in a geographically central location at the University of Connecticut Health Center (UCHC) in Farmington, CT, thereby ensuring facile access for all participating research groups. The cryo-EM will be integrated into the established UCHC EM facility, which has a proven tradition of research excellence for over 30 years. The UCHC EM facility is operated by a full-time manager and an assistant director, supported by UCHC. As typical for shared instrumentation, this instrument will be available not only to the core research groups, but also to others throughout New England that will benefit from it. This proposal combines researchers from 3 universities, with a total of 18 research groups forming the core user group. Research areas include virology, signal-transduction, immunobiology, DNA repair among many others, with key implications for a broad variety of diseases. The proposed research projects are well supported with exciting preliminary data. Many of the 18 core research groups have gained extensive cryo-EM experience over the last years and have a strong external funding portfolio. While access to high powered cryo-EM instruments is available, the access to affordable, easy-to-use screening cryo-EM’s that allow for the careful optimization of samples for structure determination are either non-existent or expensive and thus out of reach for many research groups. The requested Tundra 100 kV cryo-EM will solve this limitation, and enable rapid, cost-effective and easy access for the investigation of large, highly complex biological systems. Furthermore, it will enhance the training capabilities of students and post-doctoral research scientists for cryo-EM. In summary, the so-called ‘resolution-revolution’ has highlighted the capabilities of novel cryo-EM instruments for the structure determination of critical biological samples to answer fundamental biological questions. However, as for all structural biology techniques, sample optimization is the key essential step for gaining these new insights. The requested instrument will fill this gap for a significant number of researchers throughout New England. Further, significant institutional investments will guarantee the long-term success and will allow key biological functions to be determined. We are confident that this cryo-EM instrument will not only provide a necessary nucleus for the research of the core participating laboratories specifically but will also provide a long-lasting impact for New England, as well as the US research community, generally.

该提案请求支持购买最先进的 100 kV 筛选冷冻电镜 乐器。该仪器将安装在大学的地理位置中心位置 位于康涅狄格州法明顿的康涅狄格州健康中心 (UCHC)，从而确保所有人都能方便地使用 参与的研究小组。冷冻电镜将集成到已建立的 UCHC 电镜中 该设施拥有 30 多年来久经考验的卓越研究传统。全民健康中心 EM 该设施由一名全职经理和一名助理主任负责运营，并由 UCHC 提供支持。作为 典型的共享仪器，该仪器不仅可用于核心研究 团体，也包括整个新英格兰地区将从中受益的其他人。这个提议 联合3所大学的研究人员，共有18个研究小组组成核心用户 团体。研究领域包括病毒学、信号转导、免疫生物学、DNA 修复 许多其他疾病，对多种疾病具有重要影响。拟议的研究 项目得到了令人兴奋的初步数据的有力支持。 18个核心研究小组中的许多 在过去几年中获得了丰富的冷冻电镜经验，并拥有强大的外部资金 文件夹。虽然可以使用高功率冷冻电镜仪器，但 经济实惠、易于使用的冷冻电镜筛选，可对样品进行仔细优化 结构测定要么不存在，要么成本高昂，因此对许多人来说是遥不可及的 研究小组。所需的 Tundra 100 kV 冷冻电镜将解决这一限制，并能够 快速、经济高效且方便地研究大型、高度复杂的生物 系统。此外，还将增强学生和博士后的培养能力 冷冻电镜研究科学家。总之，所谓的“决议革命”凸显了 新型冷冻电镜仪器用于关键生物结构测定的能力 样本来回答基本的生物学问题。然而，对于所有结构生物学 技术，样本优化是获得这些新见解的关键必要步骤。这 所需的仪器将填补整个新领域大量研究人员的这一空白 英格兰。此外，大量的机构投资将保证长期的成功和 将允许确定关键的生物学功能。我们相信这款冷冻电镜仪器 不仅将为核心参与实验室的研究提供必要的核心 特别是，但也将为新英格兰以及美国的研究提供长期的影响 社区，一般而言。