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Fluorescence-based methods for microconnectivity analysis in neocortex

Fluorescence-based methods for microconnectivity analysis in neocortex
基于荧光的新皮质微连接分析方法
批准号:
10413555
负责人:
ALISON L BARTH
金额:
$159.7万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-05-01 至 2025-04-30

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中文摘要
翻译
摘要 对大脑中神经细胞类型的全面普查,以及分子遗传学 细胞类型特异性标记的资源,正在彻底改变我们检测和监测年龄的能力, 疾病和神经连接的经验依赖性变化。基于荧光 定量突触分析的方法将很容易被科学界采用 并已广泛用于神经元培养,但尚未充分开发用于分析 大脑网络的连通性,由于对检测的分辨率和准确性的关注, 接触和试剂使用困难。在这里,我们将使用最近开发的工具, 突触标记开发工作流程,以评估和减轻对荧光的担忧- 使用最先进的扩展显微镜方法在脑组织中进行基于测量。我们 然后将测试这些试剂的能力,以推动对特定输入可塑性的新见解 在学习过程中,使用一种特征良好的训练模式, 已经证实了丘脑皮质突触强度的变化。
英文摘要
ABSTRACT A comprehensive census of neural cell types in the brain, together with molecular-genetic resources for cell-type specific labeling, is revolutionizing our ability to detect and monitor age, disease, and experience-dependent changes in neural connectivity. Fluorescence-based methods for quantitative synapse analysis would be easily adopted by the scientific community and have been widely used in neuronal cultures, but have not been well-developed for analysis of connectivity in brain networks, due to concerns about resolution and accuracy of detected contacts and difficulty of reagent use. Here we will use recently-developed tools for fluorescent synaptic labeling to develop a workflow to evaluate and mitigate concerns about fluorescence- based measurements in brain tissue using state-of-the-art Expansion Microscopy methods. We will then test the ability of these reagents to drive new insights about input-specific plasticity during learning, using a well-characterized training paradigm where electrophysiological changes in thalamocortical synaptic strength have been demonstrated.
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Determinants of sparse activity in neocortex
  • 批准号:
    10375925
  • 项目类别:
  • 资助金额:
    $54.31万
  • 财政年份:
    2022
  • 负责人:
    ALISON L BARTH
  • 依托单位:
Determinants of sparse activity in neocortex
  • 批准号:
    10558601
  • 项目类别:
  • 资助金额:
    $50.87万
  • 财政年份:
    2022
  • 负责人:
    ALISON L BARTH
  • 依托单位:
Synaptic plasticity in sensory learning
  • 批准号:
    10598941
  • 项目类别:
  • 资助金额:
    $39.75万
  • 财政年份:
    2022
  • 负责人:
    ALISON L BARTH
  • 依托单位:
In Vivo Synaptic Imaging in Neocortex
  • 批准号:
    10471169
  • 项目类别:
  • 资助金额:
    $22.55万
  • 财政年份:
    2021
  • 负责人:
    ALISON L BARTH
  • 依托单位:
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