Unraveling the mechanism by which the BAM complex mediates OMP biogenesis

揭示 BAM 复合物介导 OMP 生物发生的机制

基本信息

  • 批准号:
    10415950
  • 负责人:
  • 金额:
    $ 29.63万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-07-08 至 2024-05-31
  • 项目状态:
    已结题

项目摘要

Project Summary Gram-negative bacteria, such as E. coli, have an outer membrane which provides nutrients and added protection for the cell. The outer membrane also contains proteins that can enable pathogenic bacteria to evade host immune responses and cause a number of serious infectious diseases. Despite the importance of these outer membrane proteins (OMPs) for cell survival and virulence, little is known about how these proteins are folded and inserted into the outer membrane. However, in E. coli, we know that a 200 kDa five-component complex called the β-barrel assembly machinery (BAM) complex mediates the biogenesis of these membrane proteins. In the last decade, the individual structures of components of the BAM complex have been reported with the structure of BamA, the central component, providing the most useful clues to how the BAM complex may function. Here, it was revealed that BamA may destabilize the local membrane, thereby promoting protein folding and insertion into the membrane. Recently, our work and others’ have determined the structure of the BAM complex, revealing unprecedented conformational changes within the barrel domain of BamA. Our work here will utilize these structures to study the role of the conformational changes for the function of the BAM complex in OMP biogenesis. Additionally, we will determine how substrate OMPs are recognized by the BAM complex and how they interact with BAM during folding into the membrane. Lastly, we will use structural biology methods to study the BAM complex in a lipid bilayer and in the presence of substrates. Our proposed studies will provide crucial functional and structural insight towards unraveling how the BAM complex performs its essential role in bacteria.
项目总结

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Nicholas Noinaj其他文献

Nicholas Noinaj的其他文献

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{{ truncateString('Nicholas Noinaj', 18)}}的其他基金

Unraveling the mechanism by which the BAM complex mediates OMP biogenesis
揭示 BAM 复合物介导 OMP 生物发生的机制
  • 批准号:
    9974536
  • 财政年份:
    2019
  • 资助金额:
    $ 29.63万
  • 项目类别:
Unraveling the mechanism by which the BAM complex mediates OMP biogenesis
揭示 BAM 复合物介导 OMP 生物发生的机制
  • 批准号:
    10163875
  • 财政年份:
    2019
  • 资助金额:
    $ 29.63万
  • 项目类别:
Structural Characterization of the TOC Protein Translocon Machinery
TOC 蛋白易位机的结构表征
  • 批准号:
    10376194
  • 财政年份:
    2018
  • 资助金额:
    $ 29.63万
  • 项目类别:
Structural Characterization of the TOC Protein Translocon Machinery
TOC 蛋白易位机的结构表征
  • 批准号:
    9900017
  • 财政年份:
    2018
  • 资助金额:
    $ 29.63万
  • 项目类别:
The role of BamA in the biogenesis of beta-barrel membrane proteins
BamA 在 β-桶膜蛋白生物合成中的作用
  • 批准号:
    8767875
  • 财政年份:
    2015
  • 资助金额:
    $ 29.63万
  • 项目类别:
The role of BamA in the biogenesis of beta-barrel membrane proteins
BamA 在 β-桶膜蛋白生物发生中的作用
  • 批准号:
    9110832
  • 财政年份:
    2015
  • 资助金额:
    $ 29.63万
  • 项目类别:

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