FUNCTIONAL ANALYSIS OF SLEEP HOMEOSTASIS IN DROSOPHILA

尽管睡眠的确切功能仍不清楚，但毫无疑问的是 睡眠是维持大量和不同数量的 生物系统。事实上，与年龄和年龄相关的认知障碍 神经退行性疾病常伴有睡眠生理缺陷。 和建筑。我们之前已经证明，增强的睡眠可以逆转记忆 即使在果蝇的主要记忆中心有灾难性损伤的情况下也是如此。我们有 现在绘制了这个记忆测试所需的最小电路。利用基因组学和 行为遗传学我们已经确定了当记忆恢复时可以恢复记忆的基因 以与休眠产生的方式类似的方式以单个电路组件进行调制。 在这项提案中，我们将确定调控这些基因的分子途径。在……里面 此外，我们将使用钙成像来确定如何恢复单个电路 组件通过调节其他电路组件的活动来恢复内存 脑损伤或人类阿尔茨海默病相关基因的表达。

Although the precise function of sleep remains unknown, there is little question that sleep is required for maintaining optimal performance in a large and diverse number of biological systems. Indeed, cognitive impairments associated with aging and neurodegenerative disorders are frequently accompanied by deficits in sleep physiology and architecture. We have previously shown that enhanced sleep can reverse memory deficits even in flies with catastrophic lesions to their primary memory center. We have now mapped a minimal circuitry required by this memory assay. Using genomics and behavioral genetics we have identified genes that can restore memory when they are modulated in a single circuit-component in a similar fashion as that produced by sleep. In this proposal we will determine the molecular pathways that regulate these genes. In addition, we will use calcium imaging to determine how restoring a single circuit component modulates the activity of other circuit components to restore memory during brain damage or the expression of Human Alzheimer's' related genes.