FUNCTIONAL ANALYSIS OF SLEEP HOMEOSTASIS IN DROSOPHILA

虽然睡眠的确切功能仍不清楚，但毫无疑问， 需要睡眠来在大量不同的 生物系统。事实上，与衰老相关的认知障碍， 神经退行性疾病经常伴随睡眠生理学缺陷 和建筑。我们之前已经证明，增强睡眠可以逆转记忆 即使是对主要记忆中心造成灾难性损伤的果蝇也会出现这种缺陷。我们有 现在绘制了这个记忆测定所需的最小电路。利用基因组学和 行为遗传学，我们已经确定了基因，可以恢复记忆时，他们是 以与睡眠产生的类似的方式在单个电路组件中调制。 在这个建议中，我们将确定调节这些基因的分子途径。在 此外，我们将使用钙成像来确定如何恢复一个单一的电路， 组件调节其他电路组件的活动，以恢复记忆， 脑损伤或人类阿尔茨海默氏症相关基因的表达。

Although the precise function of sleep remains unknown, there is little question that sleep is required for maintaining optimal performance in a large and diverse number of biological systems. Indeed, cognitive impairments associated with aging and neurodegenerative disorders are frequently accompanied by deficits in sleep physiology and architecture. We have previously shown that enhanced sleep can reverse memory deficits even in flies with catastrophic lesions to their primary memory center. We have now mapped a minimal circuitry required by this memory assay. Using genomics and behavioral genetics we have identified genes that can restore memory when they are modulated in a single circuit-component in a similar fashion as that produced by sleep. In this proposal we will determine the molecular pathways that regulate these genes. In addition, we will use calcium imaging to determine how restoring a single circuit component modulates the activity of other circuit components to restore memory during brain damage or the expression of Human Alzheimer's' related genes.