Shared Resource 06: DNA Resource

共享资源06：DNA资源

• 批准号: 10062893
• 负责人: STEPHANIE E MOHR
• 金额: $ 24.14万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-10 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10062893
• 关键词: Address; Agar; Automation; Basic Science; Cancer Center; Cancer Center Support Grant; Cellular biology; Cloning; Code; Collection; Complementary DNA; Consultations; Cosmids; Custom; DNA; DNA Repository; DNA sequencing; Dana-Farber Cancer Institute; Drosophila genus; Escherichia coli; Experimental Designs; Funding; Genes; Genomics; High-Throughput DNA Sequencing; Home; Hour; Human; Informatics; Institution; Knowledge; Libraries; Liquid substance; Malignant Neoplasms; Mission; Molecular; Mutagenesis; Pathway interactions; Plasmids; Preparation; Proteins; Proteome; RNA Interference; Reaction; Remote Consultation; Research Personnel; Resource Sharing; Resources; Robotics; Sampling; Services; Site; Techniques; Training and Education; Translational Research; Validation; Xenopus; Yeasts; cost; design; interest; medical schools; member; plasmid DNA; posters; professor; repository; service member; success; tool; user-friendly

DNA Resource Shared Resource Project Summary / Abstract The DNA Resource Core provides investigators access to specialized resources such as informatics support, rapid and user-friendly high-throughput DNA sequencing services, and cDNA/RNAi clone repository and distribution services. The success of the Core is evidenced by its usage, sequencing about 200,000 samples per year. The Core's PlasmID DNA repository is curating 336,004 plasmid DNA clones useful for cloning, mutagenesis, and expression relevant to more than 25 different species, with the emphasis on human cancer- related gene collections. The Core distributes approximately 7,000 clones to researchers on an annual basis.

DNA资源共享资源 项目摘要 /摘要 DNA Resource Core使研究人员可以访问专业资源，例如信息学支持， 快速且用户友好的高通量DNA测序服务以及cDNA/RNAi克隆存储库和 分销服务。核心的成功可以通过其使用来证明，测序约200,000个样本 每年。核心的质粒DNA存储库正在策划336,004质粒DNA克隆，可用于克隆， 诱变，表达与超过25种不同的物种相关，重点是人类癌 - 相关基因收集。核心每年向研究人员分配大约7,000个克隆。