Shared Resource 05: Collaborative Functional Genomics

共享资源 05：协作功能基因组学

• 批准号: 10062890
• 负责人: STEPHANIE E MOHR
• 金额: $ 14.97万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-10 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10062890
• 关键词: Automation; Bioinformatics; Biological; Biological Assay; CRISPR/Cas technology; Cancer Center; Categories; Cells; Cellular biology; Chemistry; Clustered Regularly Interspaced Short Palindromic Repeats; Code; Communities; Consultations; Dana-Farber Cancer Institute; Data; Data Analyses; Development; Doctor of Philosophy; Drosophila genus; Education; Educational workshop; Equipment; Fluorescence Microscopy; Funding; Gene set enrichment analysis; Generations; Genes; Genetic; Genome engineering; Genomic approach; Genomics Shared Resource; Group Meetings; Guide RNA; Home; Human; Image; Imaging Device; Institutes; Institution; Knock-out; Laser Scanning Cytometry; Libraries; Liquid substance; Luciferases; Malignant Neoplasms; MicroRNAs; Microscopy; Mission; Mus; Open Reading Frames; Output; Pathway interactions; Positioning Attribute; Production; Proteins; RNA Interference; RNA interference screen; Reagent; Reporter; Research Personnel; Resource Sharing; Resources; Running; Services; Site; Small Interfering RNA; Technology; Training; Training and Education; Triage; Untranslated RNA; Validation; Visualization; assay development; base; cancer prevention; cancer therapy; data quality; design; experience; functional genomics; gain of function; genetic approach; genome-wide; high throughput screening; improved; in vivo; instrument; loss of function; medical schools; meetings; member; overexpression; prospective; protein complex; screening; service member; small hairpin RNA; tumor progression

Collaborative Functional Genomics Shared Resource Project Summary / Abstract Understanding the basic biological mechanisms that underlie the development and progression of cancer is fundamental to finding better approaches to cancer prevention and treatment. Loss-of-function genetic approaches, such as high-throughput RNA interference (RNAi) and CRISPR/Cas9, as well as gain-of-function approaches such as open reading frame (ORF) over-expression and CRISPR-activation (CRISPR-a) provide cutting-edge strategies to identify new cancer-related genes and pathways. The Collaborative Functional Genomics Core offers a single portal through which DF/HCC members gain access to technologies, reagents, services, and scientific consultation. The Core mission is to support the widest possible range of functional genomics screening projects; rapidly disseminate information about new functional genomics approaches; and help researchers navigate from concept and assay development to high-throughput screening, analysis, and validation.

功能基因组学协作共享资源 项目总结/摘要 了解癌症发生和进展的基本生物学机制是 这是找到更好的癌症预防和治疗方法的基础。功能丧失遗传 方法，如高通量RNA干扰（RNAi）和CRISPR/Cas9，以及功能获得 诸如开放阅读框（ORF）过表达和CRISPR-活化（CRISPR-a）的方法提供了 确定新的癌症相关基因和途径的尖端策略。合作功能 Genomics Core提供了一个单一的门户网站，DF/HCC成员可以通过该门户网站获得技术、试剂 服务和科学咨询。核心使命是支持尽可能广泛的功能 基因组学筛选项目;迅速传播关于新的功能基因组学方法的信息;以及 帮助研究人员从概念和检测开发到高通量筛选、分析， 验证。