Combined bioprinting with stem cell technology to regenerate skin burn wounds

生物打印与干细胞技术相结合，使皮肤烧伤创面再生

• 批准号: 10447161
• 负责人: Marco C Bottino
• 金额: $ 50.02万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10447161
• 关键词: 3-Dimensional; Address; Affect; American; Animals; Behavior; Biocompatible Materials; Biological; Biological Testing; Biology; Body Fluids; Burn injury; Cell Proliferation; Cells; Cessation of life; Chemicals; Connective Tissue; Dehydration; Effectiveness; Engineering; Epidermis; Epithelial; Epithelial Cells; Extracellular Matrix Proteins; FRAP1 gene; Family suidae; Fibroblasts; Gelatin; Genetic Transcription; Harvest; Heterogeneity; Homeostasis; Housing; Human; Hydrogels; Infection; Injury; Lifting; Liquid substance; Mediating; Mesenchyme; Michigan; Modification; Molecular; Nanosphere; Organ; Osteoblasts; Pathway interactions; Patients; Play; Polymers; Process; Quality of life; Recombinant Proteins; Recombinants; Recovery; Research; Signal Transduction; Site; Skin; Stratum Basale; Structure; Suspensions; Technology; Testing; Therapeutic; Time; Tissue Donors; Tissue Engineering; Translations; Universities; Work; base; biodegradable scaffold; biofabrication; bioink; bioprinting; burn model; burn wound; caprolactone; cell motility; design; diabetic ulcer; epidermal stem cell; healing; improved; in vivo; innovation; melting; novel; novel therapeutic intervention; novel therapeutics; organ growth; prevent; recruit; response; scaffold; severe injury; single cell sequencing; skin barrier; skin regeneration; stem cell technology; stem cells; therapeutic effectiveness; tissue regeneration; translation to humans; wound; wound closure; wound healing

PROJECT SUMMARY The skin (epidermis) is responsible for protecting the body and works as a specialized, vital defensive barrier. Upon injury, the immediate response of the epidermis and its stem cells aims to reestablish local homeostasis. Extensive injuries, often observed in burn patients, compromise the epidermal homeostasis, which leads to dehydration, possible severe infections, and death. New therapeutic strategies designed to improve healing and burn injuries are critically needed. Our translation-focused innovative strategy aims to develop mechanism-based and material engineering-driven therapies to augment epidermal healing. Here, we are developing novel and exciting biodegradable scaffolds for the controlled delivery of key recombinant proteins and cells to positively reestablish an epidermal barrier and wound closure. We propose to study how this novel therapeutic strategy affects the skin stem cells and wound healing process. Furthermore, we will determine the therapeutic effectiveness of 3D tissue engineering on burn wounds using bioprinting technology. Our positive results will improve the mechanistic understanding of how patient-originated stem cells and epidermal cells along with 3D bioprinting contribute to the healing of burn wounds. By accomplishing these specific aims, we will aid in the management of burn patients, using limited donor tissues and help to reduce burn patients’ recovery time.

项目总结 皮肤(表皮)负责保护身体，是一种特殊的、重要的防御屏障。 损伤后，表皮及其干细胞的即时反应旨在重建局部内环境平衡。 烧伤患者常可观察到的大面积损伤会破坏表皮内环境的稳定，从而导致 脱水，可能的严重感染，以及死亡。新的治疗策略旨在改善愈合和 烧伤是当务之急。我们以翻译为中心的创新战略旨在发展基于机制的 以及材料工程驱动的疗法，以促进表皮愈合。在这里，我们正在开发新的和 激动型生物可降解支架控制关键重组蛋白和细胞的传递 重建表皮屏障并缝合伤口。我们打算研究这种新的治疗策略是如何 影响皮肤干细胞和伤口愈合过程。此外，我们将确定治疗方案 生物打印技术在烧伤创面三维组织工程中的应用效果。我们的积极成果将 提高对患者起源的干细胞和表皮细胞以及3D的机制的理解 生物打印有助于烧伤创面的愈合。通过实现这些具体目标，我们将帮助 管理烧伤患者，使用有限的供体组织，有助于缩短烧伤患者的恢复时间。