Combined bioprinting with stem cell technology to regenerate skin burn wounds

项目摘要 皮肤（表皮）负责保护身体，并作为一个专门的，重要的防御屏障。 损伤后，表皮及其干细胞的立即反应旨在重建局部稳态。 在烧伤患者中经常观察到的大面积损伤损害了表皮的稳态，这导致 脱水可能严重感染甚至死亡新的治疗策略旨在改善愈合和 严重烧伤的病人是急需的。我们以预防为重点的创新战略旨在开发基于机制的 和材料工程驱动的疗法来增强表皮愈合。在这里，我们正在开发新的， 令人兴奋的生物可降解支架，用于控制关键重组蛋白和细胞的递送， 重建表皮屏障和伤口闭合。我们建议研究这种新的治疗策略如何 影响皮肤干细胞和伤口愈合过程。此外，我们将确定治疗 使用生物打印技术的3D组织工程对烧伤创面的有效性。我们的积极成果将 提高了对患者来源的干细胞和表皮细胞如何沿着与3D 生物打印有助于烧伤伤口的愈合。通过实现这些具体目标，我们将帮助 管理烧伤病人，使用有限的供体组织，并有助于减少烧伤病人的康复时间。

PROJECT SUMMARY The skin (epidermis) is responsible for protecting the body and works as a specialized, vital defensive barrier. Upon injury, the immediate response of the epidermis and its stem cells aims to reestablish local homeostasis. Extensive injuries, often observed in burn patients, compromise the epidermal homeostasis, which leads to dehydration, possible severe infections, and death. New therapeutic strategies designed to improve healing and burn injuries are critically needed. Our translation-focused innovative strategy aims to develop mechanism-based and material engineering-driven therapies to augment epidermal healing. Here, we are developing novel and exciting biodegradable scaffolds for the controlled delivery of key recombinant proteins and cells to positively reestablish an epidermal barrier and wound closure. We propose to study how this novel therapeutic strategy affects the skin stem cells and wound healing process. Furthermore, we will determine the therapeutic effectiveness of 3D tissue engineering on burn wounds using bioprinting technology. Our positive results will improve the mechanistic understanding of how patient-originated stem cells and epidermal cells along with 3D bioprinting contribute to the healing of burn wounds. By accomplishing these specific aims, we will aid in the management of burn patients, using limited donor tissues and help to reduce burn patients’ recovery time.