A Novel Multilayered Membrane for Periodontal Regeneration

用于牙周再生的新型多层膜

• 批准号: 9527438
• 负责人: Marco C Bottino
• 金额: $ 13.37万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9527438
• 关键词: Adhesions; Adult; Affect; Alkaline Phosphatase; Alveolar; Award; Biocompatible Materials; Biology; Bone Density; Bone Regeneration; Bone Surface; Bone Tissue; Canis familiaris; Cell Count; Cell Culture Techniques; Cell Survival; Cellular biology; Cementoblast; Chronic; Clinical; Clinical Trials; Connective Tissue; Cues; Defect; Dental; Dental Cementum; Dentists; Development; Development Plans; Diagnostic radiologic examination; Disease; Effectiveness; Environment; Epithelial Attachment; Evaluation; Extracellular Matrix Proteins; FGF2 gene; Fibroblast Growth Factor; Fibroblasts; Foundations; Funding; Goals; Gray unit of radiation dose; Growth and Development function; Guided Tissue Regeneration; Height; Human; Implant; In Vitro; Inflammatory; Knowledge; Laboratories; Lead; Left; Literature; Location; Mandible; Measurement; Measures; Membrane; Mentors; Mesenchymal Stem Cells; Methodology; Methods; Minerals; Modeling; Molecular Biology; Molecular Profiling; Molecular and Cellular Biology; Nanotechnology; Natural regeneration; Operative Surgical Procedures; Oral; Osteoblasts; Osteogenesis; Patient Care; Periodontal Ligament; Periodontitis; Polytetrafluoroethylene; Positioning Attribute; Principal Investigator; Procedures; Research; Resources; Scientist; Secure; Side; Signal Transduction; Stem cells; Supervision; Surface; Techniques; Tissue Engineering; Tissues; Tooth Ankylosis; Tooth Loss; Tooth root structure; Tooth structure; Training; Translating; Translational Research; aging population; alveolar bone; biomaterial development; bone; calcium phosphate; career development; clinical practice; craniofacial; design; experimental study; histological image; implantation; in vitro Assay; in vivo; innovation; migration; mineralization; multidisciplinary; novel; osteoblast differentiation; programs; public health relevance; regenerative; regenerative therapy; response; skeletal; skills; socioeconomics; soft tissue; tissue reconstruction; tissue regeneration

DESCRIPTION (provided by applicant): With the US population aging, it is important to find novel dental biomaterials that will promote periodontal regeneration, and translate these to clinical practice. As both a dentist and materials scientist, the applicant is in a position to brige the gap between biomaterials development. and their application in patient care using nanotechnology, tissue engineering and clinical knowledge. The purpose of this award is to provide the principal investigator extensive training in cellular and molecular biology, skeletal biology, and translational research with an emphasis on periodontal tissue regeneration. The experiments in this application have been designed to incorporate new methodologies, and will be guided by a multidisciplinary team of experts who will provide the required training to advance Dr. Bottino's scientific technical and analytic skills. The principal investigator's ultimae goal is to become a productive and independent clinician-scientist who can secure federal funding to sponsor an independent research program. The central hypothesis of the proposed research is that a multilayer membrane capable of stimulating the regeneration of various tissue types in a location specific manner, through the addition of calcium phosphate and fibroblast growth factor-2 (CaP/FGF2) and FGF2, will lead to alveolar and periodontal tissue regeneration in the appropriate locations. The literature indicates that no single implantable biomaterial can consistently guide the coordinated growth and development of multiple tissue types, especially in very large periodontal defects. Thus, there is a critical need to develop regenerative therapies to promote oral and craniofacial tissue regeneration and reconstruction. The specific aims of the proposed research are (1) To use an in vitro assay model to maximize the tissue-specific regenerative capacity of the multilayered implant surface layers, (2) To assess the effectiveness of CaP/FGF2 on bone regeneration in an alveolar defect using an in vivo canine model, and (3) To assess the effectiveness of the multilayered implant to regenerate soft and mineralized tissues in a location-specific manner using an in vivo canine model. The principal investigator has devised a comprehensive career development plan that includes formal coursework, laboratory guidance to cover intensive cell and molecular biology training, as well as in vivo techniques combined with a diverse set of bone/periodontal tissue regeneration related analyses under the supervision of his mentoring team. Collectively, the K08 award has the capacity to provide the resources and training needed to give the applicant the foundation and critical theoretical and practical means to become a highly qualified clinician-scientist who can translate his knowledge of biomaterials to clinical practice.

描述（由申请人提供）：随着美国人口老龄化，寻找能够促进牙周再生的新型牙科生物材料并将其转化为临床实践是非常重要的。作为一名牙医和材料科学家，申请人的职位是弥合生物材料发展之间的差距。以及它们在病人护理中的应用，利用纳米技术、组织工程和临床知识。该奖项的目的是为首席研究员提供细胞和分子生物学、骨骼生物学和转译研究方面的广泛培训，重点是牙周组织再生。本应用程序中的实验旨在采用新的方法，并将由一个多学科专家小组指导，该小组将提供所需的培训，以提高Bottino博士的科学技术和分析技能。首席研究员的最终目标是成为一名多产的、独立的临床科学家，能够获得联邦资金来赞助一个独立的研究项目。本研究的中心假设是，通过添加磷酸钙和成纤维细胞生长因子-2 （CaP/FGF2）和FGF2，多层膜能够以特定位置的方式刺激各种组织类型的再生，从而导致牙槽和牙周组织在适当的位置再生。文献表明，没有一种单一的可植入生物材料能够持续地引导多种组织类型的协调生长和发育，特别是在非常大的牙周缺损中。因此，迫切需要开发再生疗法

项目成果

Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function.

三抗抗生素聚合物纳米纤维用于纳米药物递送：对双重物种生物膜和细胞功能的影响。

• DOI: 10.1016/j.joen.2016.07.019
• 发表时间: 2016-10
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Pankajakshan D;Albuquerque MT;Evans JD;Kamocka MM;Gregory RL;Bottino MC
• 通讯作者: Bottino MC

Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm.

• DOI: 10.1016/j.joen.2015.03.005
• 发表时间: 2015-08
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Albuquerque MT;Ryan SJ;Münchow EA;Kamocka MM;Gregory RL;Valera MC;Bottino MC
• 通讯作者: Bottino MC

Antimicrobial Efficacy of Triple Antibiotic-eluting Polymer Nanofibers against Multispecies Biofilm.

• DOI: 10.1016/j.joen.2017.06.009
• 发表时间: 2017-09
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Albuquerque MTP;Nagata J;Bottino MC
• 通讯作者: Bottino MC

Dimensionally stable and bioactive membrane for guided bone regeneration: An in vitro study.

• DOI: 10.1002/jbm.b.33430
• 发表时间: 2016-04
• 期刊: Journal of biomedical materials research. Part B, Applied biomaterials
• 作者: Rowe MJ;Kamocki K;Pankajakshan D;Li D;Bruzzaniti A;Thomas V;Blanchard SB;Bottino MC
• 通讯作者: Bottino MC

Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.

• DOI: 10.1007/s00784-017-2215-y
• 发表时间: 2018-04
• 期刊: Clinical oral investigations
• 影响因子: 3.4
• 作者: Palasuk J;Windsor LJ;Platt JA;Lvov Y;Geraldeli S;Bottino MC
• 通讯作者: Bottino MC