Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication Approach

牙周组织再生的个性化策略——融合生物制造方法

• 批准号: 10668946
• 负责人: Marco C Bottino
• 金额: $ 50.11万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668946
• 关键词: 3-Dimensional; Address; Adopted; Affect; American; Animal Model; Architecture; Bacteria; Biocompatible Materials; Biological Process; Bone Growth; Ceramics; Chemicals; Chronic; Clinical; Data; Defect; Dental Cementum; Dentition; Development; Disease; Engineering; Fiber; Genes; Goals; Growth and Development function; In Vitro; Inflammatory; Ink; Lasers; Left; Magnesium; Mechanics; Medicine; Morphology; Mouth Diseases; Nanostructures; Natural regeneration; Nature; Patients; Periodontal Ligament; Periodontic specialty; Periodontitis; Physiological; Polymers; Printing; Role; Silver; Structure; Surface; Technology; Therapeutic; Tissues; Tooth Extraction; Tooth Loss; Tracheobronchomalacia; Translating; Translations; alveolar bone; antimicrobial; biofabrication; bone; bone engineering; bone loss; bone scaffold; calcium phosphate coating; canine model; clinical efficacy; clinically relevant; design; efficacy evaluation; fabrication; human old age (65+); improved; in vivo; in vivo Model; innovation; inorganic phosphate; manufacture; melting; novel; personalized medicine; personalized strategies; regenerative therapy; scaffold; tissue regeneration; tool

” Periodontitis (gum disease) is a ubiquitous chronic inflammatory, bacteria-triggered oral disorder affecting two-thirds of Americans over age 65. If left untreated, it leads to severe destruction of the periodontal attachment apparatus, eventually resulting in tooth loss. There are no approaches for predictably regenerating defects with severe bone loss and avoiding tooth extraction. Thus, there is an emerging quest for personalized solutions that can guide coordinated growth and development of the periodontal attachment apparatus. We hypothesize that: (1) Melt ElectroWriting (MEW) is the fabrication tool required to develop a compositionally and structurally tailored, tissue-specific scaffold to hearten bone growth and PDL formation on root surfaces with moderate adjacent bone; and (2) the convergence of MEW with ceramic printing is key to engineering a compositionally graded and structurally tailored scaffold to support bone growth while guiding PDL formation on root surfaces with minimum adjacent bone. Two aims are proposed. Aim 1 will optimize a personalized, compositionally and structurally tailored, tissue-specific polymeric scaffold to regenerate defects with moderate surrounding bone. Aim 2 will develop a personalized, compositionally graded and structurally tailored, tissue-specific multi-material scaffold to regenerate defects with minimum surrounding bone. To expedite translation, we will determine efficacy using our clinically relevant animal models. Successful completion of this project is central to translating personalized therapies to treat tissue destruction caused by periodontitis and thus saving millions of teeth from extraction.

” 牙周炎（牙龈疾病）是一种普遍存在的慢性炎症，细菌引发的口腔疾病，影响三分之二的65岁以上的美国人。如果不及时治疗，它会导致牙周附着装置的严重破坏，最终导致牙齿脱落。目前还没有可预测的方法来再生严重骨质流失的缺损并避免拔牙。因此，出现了对能够引导牙周附着装置的协调生长和发育的个性化解决方案的需求。我们假设：（1）熔融电写入（MEW）是开发组成和结构定制的组织特异性支架所需的制造工具，以促进具有中等相邻骨的牙根表面上的骨生长和PDL形成;以及（2）MEW与陶瓷打印的融合是设计成分分级和结构定制支架的关键，以支持骨生长，同时引导PDL在牙根表面形成，邻近的骨头。提出了两个目标。目标1将优化个性化的，成分和结构定制的，组织特异性的聚合物支架，以再生具有适度周围骨的缺损。目标2将开发一种个性化的，成分分级和结构定制的，组织特异性的多材料支架，以最小的周围骨再生缺损。为了加快翻译，我们将使用临床相关的动物模型确定疗效。该项目的成功完成对于将个性化疗法转化为治疗牙周炎引起的组织破坏，从而挽救数百万颗牙齿的拔除至关重要。