Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication Approach

“ 牙周炎(牙周病)是一种普遍存在的慢性炎症性口腔疾病，由细菌引发，影响着三分之二65岁以上的美国人。如果不治疗，它会导致牙周附着装置的严重破坏，最终导致牙齿脱落。目前尚无方法可预测地再生严重骨质丢失的缺损区，避免拔牙。因此，出现了对能够指导牙周附着装置的协调生长和发展的个性化解决方案的探索。我们假设：(1)熔融电写(MEW)是开发成分和结构量身定制的组织特异性支架所需的制造工具，以鼓励骨生长和在具有适度邻接骨的根面上形成PDL；以及(2)MEW与陶瓷印刷的融合是设计成分分级和结构量身定制的支架的关键，以支持骨生长，同时引导具有最少邻接骨的根面上PDL的形成。提出了两个目标。目标1将优化一种个性化、成分和结构量身定做的组织特异性聚合物支架，以再生具有适度周围骨的缺损区。Aim 2将开发一种个性化的、成分分级的、结构定制的、特定于组织的多材料支架，以最小的周围骨再生缺陷。为了加快翻译速度，我们将使用我们的临床相关动物模型来确定疗效。这一项目的成功完成对于将个人化疗法转化为治疗牙周炎造成的组织破坏，从而使数百万颗牙齿免于拔牙至关重要。

” Periodontitis (gum disease) is a ubiquitous chronic inflammatory, bacteria-triggered oral disorder affecting two-thirds of Americans over age 65. If left untreated, it leads to severe destruction of the periodontal attachment apparatus, eventually resulting in tooth loss. There are no approaches for predictably regenerating defects with severe bone loss and avoiding tooth extraction. Thus, there is an emerging quest for personalized solutions that can guide coordinated growth and development of the periodontal attachment apparatus. We hypothesize that: (1) Melt ElectroWriting (MEW) is the fabrication tool required to develop a compositionally and structurally tailored, tissue-specific scaffold to hearten bone growth and PDL formation on root surfaces with moderate adjacent bone; and (2) the convergence of MEW with ceramic printing is key to engineering a compositionally graded and structurally tailored scaffold to support bone growth while guiding PDL formation on root surfaces with minimum adjacent bone. Two aims are proposed. Aim 1 will optimize a personalized, compositionally and structurally tailored, tissue-specific polymeric scaffold to regenerate defects with moderate surrounding bone. Aim 2 will develop a personalized, compositionally graded and structurally tailored, tissue-specific multi-material scaffold to regenerate defects with minimum surrounding bone. To expedite translation, we will determine efficacy using our clinically relevant animal models. Successful completion of this project is central to translating personalized therapies to treat tissue destruction caused by periodontitis and thus saving millions of teeth from extraction.