Flow Cytometry Core

摘要 流式细胞仪核心(核心B)有四个主要目标。它的主要功能是提供最先进的 流式细胞术分析细胞抗原表达、功能、代谢和功能的设备和专业知识 从而支持路易斯维尔大学在糖尿病和肥胖症方面的心血管研究。堆芯 B人员是经验丰富的调查人员，他们在流式细胞术方面工作了几年，使用 来自多个物种的各种组织和细胞，并在罕见事件分析方面拥有广泛的专业知识。这个 他们的背景和专业领域的互补性，加上他们的合议性，提供了 为核心内部的富有成效的协同及其与其他调查人员的富有成效的互动奠定坚实的基础 在DOC、UOFL以及与其他研究所的协作互动中。其次，核心是致力于 达到一个教育目标。不仅将对所有用户进行仪器操作方面的培训，而且其目的是 用户完全了解细胞学的原理、应用和数据分析。通过实践培训和 教育演示文稿，希望所有当前和未来的用户都能够胜任地将 流式细胞术作为分析工具。第三，核心致力于发展其范围和运营，以支持 多维流式细胞术是未来发展的需要。它将继续升级其设备和分析 曲目。最后，这个核心的最终目标是发展成为一个自给自足的大规模流程 不仅满足中心的特定需求，而且满足其他大学和 对糖尿病和肥胖症研究感兴趣的校外调查人员。一种完全开发的流式细胞仪核心 将是开发未来多调查员项目和帮助研究人员进行 旨在改善糖尿病造成的健康危害的多方面基础和临床研究 还有肥胖症。

ABSTRACT The Flow Cytometry Core (Core B) has four major goals. Its primary function is to provide state of the art equipment and expertise for flow cytometric analysis of cellular antigen expression, function, metabolism and signaling thereby supporting cardiovascular research in diabetes and obesity at the University of Louisville. Core B personnel are highly-experienced investigators who have worked for several years on flow cytometry using various tissues and cells from multiple species and have extensive expertise in rare event analysis. The complementary nature of their backgrounds and areas of expertise, coupled with their collegial nature, provides a strong foundation for productive synergy within this Core and its productive interactions with other investigators in the DOC, at the UofL and with collaborative interactions with other Institutes. Secondly, the Core is committed to an educational goal. Not only will all users be trained on the operation of instruments, it is the intent that these users completely understand cytometric theory, application, and data analysis. Through hands-on training and educational presentations, it is hoped that all current and future users will be able to competently incorporate flow cytometry as an analytical tool. Third, the Core is committed to developing its scope and operation to support multidimensional flow cytometry needs in the future. It will continue to upgrade its equipment and analytical repertoire. Finally, this Core has the ultimate goal of developing into a self-supporting and large-scale flow cytometry operation that will cater not only to the specific needs of the Center but to other University and extramural investigators interested in diabetes and obesity research. A fully developed Flow Cytometry Core will be a significant asset in developing future multi-investigator projects and for helping researchers conduct multifaceted basic and clinical investigations aimed at the amelioration of health hazards caused by diabetes and obesity.