Flow Cytometry Core

摘要 流式细胞术核心（核心B）有四个主要目标。其主要功能是提供最先进的 用于流式细胞术分析细胞抗原表达、功能、代谢和 信号，从而支持路易斯维尔大学的糖尿病和肥胖症的心血管研究。核心 B人员是经验丰富的研究人员，他们在流式细胞术方面工作了数年， 来自多个物种的各种组织和细胞，并在罕见事件分析方面拥有丰富的专业知识。的 他们的背景和专业领域的互补性，加上他们的合议性质， 在本核心内进行富有成效的协同作用以及与其他研究人员进行富有成效的互动的坚实基础 在DOC，在UofL和与其他研究所的合作互动。第二，核心承诺 to an educational教育goal目标.不仅所有用户都将接受仪器操作培训， 用户完全了解细胞计数理论、应用和数据分析。通过实践培训， 教育介绍，希望所有当前和未来的用户将能够胜任地纳入 流式细胞术作为分析工具。第三，核心致力于发展其范围和业务，以支持 流式细胞仪在未来的需求。它将继续更新其设备和分析 保留曲目。最后，这个Core的最终目标是发展成为一个自收自支的大流量 细胞计数操作，不仅满足中心的具体需求，而且满足其他大学和 对糖尿病和肥胖症研究感兴趣的校外研究人员。完全开发的流式细胞术核心 将是开发未来多研究者项目和帮助研究人员进行 旨在改善糖尿病引起的健康危害的多方面基础和临床研究 和肥胖。

ABSTRACT The Flow Cytometry Core (Core B) has four major goals. Its primary function is to provide state of the art equipment and expertise for flow cytometric analysis of cellular antigen expression, function, metabolism and signaling thereby supporting cardiovascular research in diabetes and obesity at the University of Louisville. Core B personnel are highly-experienced investigators who have worked for several years on flow cytometry using various tissues and cells from multiple species and have extensive expertise in rare event analysis. The complementary nature of their backgrounds and areas of expertise, coupled with their collegial nature, provides a strong foundation for productive synergy within this Core and its productive interactions with other investigators in the DOC, at the UofL and with collaborative interactions with other Institutes. Secondly, the Core is committed to an educational goal. Not only will all users be trained on the operation of instruments, it is the intent that these users completely understand cytometric theory, application, and data analysis. Through hands-on training and educational presentations, it is hoped that all current and future users will be able to competently incorporate flow cytometry as an analytical tool. Third, the Core is committed to developing its scope and operation to support multidimensional flow cytometry needs in the future. It will continue to upgrade its equipment and analytical repertoire. Finally, this Core has the ultimate goal of developing into a self-supporting and large-scale flow cytometry operation that will cater not only to the specific needs of the Center but to other University and extramural investigators interested in diabetes and obesity research. A fully developed Flow Cytometry Core will be a significant asset in developing future multi-investigator projects and for helping researchers conduct multifaceted basic and clinical investigations aimed at the amelioration of health hazards caused by diabetes and obesity.