Core C: Cardiovascular Pathology Core

核心 C：心血管病理学核心

• 批准号: 8595398
• 负责人: Timothy E O'Toole
• 金额: $ 65.9万
• 依托单位: AMERICAN HEART ASSOCIATION
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-19 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8595398
• 关键词: Address; Alanine Transaminase; Albumins; Alkaline Phosphatase; American Heart Association; Animals; Area; Aspartate Transaminase; Basophils; Bilirubin; Biochemical; Biological Assay; Biological Markers; Blood Cells; Blood Glucose; Blood Platelets; Blood Urea Nitrogen; Blood specimen; Calcium Chloride; Cardiovascular Pathology; Cardiovascular system; Cells; Charge; Chemicals; Chemistry; Cholesterol; Chronic; Clinical Chemistry; Color; Cotinine; Creatinine; Electrolytes; Elements; Equipment; Erythrocytes; Euthanasia; Evaluation; Fatty Acids; Finding of Mean Corpuscular Hemoglobin; Glucose; Glycosylated hemoglobin A; Goals; HDL-triglyceride; Health Sciences; Hematocrit procedure; Hematology; Hemoglobin; Hepatic; Histology; Housing; Human; Human Resources; Image; Immunohistochemistry; Injury; Insulin; Ions; Ketones; Kidney; Leukocytes; Lipids; Lipoproteins; Liver Function Tests; Low-Density Lipoproteins; Lymphocyte; Mass Fragmentography; Measurement; Measures; Microscope; Microscopic; Mus; Nitrogen; Nonesterified Fatty Acids; Particle Size; Physiological; Plasma; Potassium Phosphate; Procedures; Proteins; Reader; Regulation; Renal function; Reporting; Research; Research Personnel; Resources; Sampling; Serum Albumin; Services; Smoke; Sodium; Specific Gravity; Techniques; Tobacco; Toxicology; Urea; Urinalysis; Urine; Width; addiction; base; cardiovascular injury; cytokine; eosinophil; fast protein liquid chromatography; human subject; mean corpuscular volume observed; mercapturate; monocyte; neutrophil; novel

CORE STRUCTURE The Cardiovascular Pathology Core (Core C) will provide unified and validated measurements of several biomarkers of cardiovascular injury for Center Projects. Using state-of-the-art techniques, this Core has already established Standard Operating Procedures (SOPs) for the quantification of a range of biochemicals, metabolites, cytokines, and common and rare blood cells. While it is charged to address the existing needs of the current projects, the scope of the Core services will not be limited to the requirements of these projects. ]t will refine ongoing areas of exploration and strive for developing novel assays to expand its repertoire. The fertile and dynamic resources of the Core and the technical excellence of its investigators in cardiovascular pathology and toxicology will also enable other Tobacco Research Centers to interface with A-TRAC. The Core will be housed in the Baxter II building in the Health Sciences Center of UofL. The Core currently has state of the art equipment for all its needs. Among the flow cytometers, the Core has an LSR II (Becton Dickinson; San Jose, CA) an Acuri C6 II (Becton Dickinson) and a MoFlo Legacy cell sorter (Dako, Carpinteria, CA). Other essential equipment include a Cobas-Mira Plus chemistry analyzer, a CellDyn hematology analyzer, a Chrono-log aggregometer, a BioTek SynergyMX plate reader, and a Zeiss LSM 510 confocal microscope (for details of all equipment, see Resources). Additional equipment for histology, immunohistochemistry and microscopic imaging are also available to Core personnel.

芯结构 心血管病理学核心（核心C）将为中心项目提供几种心血管损伤生物标志物的统一和经验证的测量结果。使用最先进的技术，该核心已经建立了标准操作规程（SOP），用于定量一系列生化物质，代谢物，细胞因子以及常见和罕见血细胞。虽然核心服务的收费是为了满足当前项目的现有需求，但其范围将不限于这些项目的需求。[]t将完善正在进行的探索领域，并努力开发新的测定方法，以扩大其库。核心研究中心丰富而有活力的资源及其研究人员在心血管病理学和毒理学方面的卓越技术也将使其他烟草研究中心能够与A-TRAC对接。核心将被安置在UofL健康科学中心的巴克斯特II大楼内。 核心目前拥有满足其所有需求的最先进设备。在流式细胞仪中，Core具有LSR II（Becton Dickinson;圣何塞，CA）、Acuri C6 II（Becton Dickinson）和MoFlo Legacy细胞分选仪（Dako，Carpinteria，CA）。其他基本设备包括Cobas-Mira Plus化学分析仪、CellDyn血液分析仪、Chrono-log聚集计、BioTek SynergyMX读板器和Zeiss LSM 510共聚焦显微镜（有关所有设备的详细信息，请参阅参考资料）。核心人员还可使用组织学、免疫组织化学和显微镜成像的其他设备。