Metabolism and Toxicity Core

代谢和毒性核心

• 批准号: 10354696
• 负责人: Timothy E O'Toole
• 金额: $ 31.73万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10354696
• 关键词: Address; Analytical Chemistry; Animals; Bile Acids; Biochemical; Biological Assay; Biological Markers; Biology; Blood; Bone Marrow; Cardiometabolic Disease; Cardiovascular system; Catecholamines; Cells; Chemical Exposure; Clinical; Clinical Pathology; Communities; Complex; Continuing Education; Data; Data Analyses; Data Reporting; Deposition; Detection; Development; Ensure; Environmental Exposure; Environmental Health; Equipment; Exposure to; Extramural Activities; Faculty; Felis catus; Flow Cytometry; Foundations; Functional disorder; Future; Goals; Growth; Health; Hepatic; Hepatotoxicity; Histologic; Human; Human Resources; Individual; Infrastructure; Injury; Laboratories; Mass Fragmentography; Measurement; Measures; Metabolic; Metabolism; Methodology; Methods; Mus; Pathologic; Pathology; Physiological; Population; Postdoctoral Fellow; Procedures; Property; Protocols documentation; Quality Control; Research; Research Personnel; Research Project Grants; Resolution; Resources; Risk; Sampling; Scientist; Services; Signal Transduction; Students; Superfund; Techniques; Technology; Testing; Toxic effect; Toxicology; Training; Universities; Urine; blood glucose regulation; cardiometabolism; career development; data dictionary; data harmonization; data management; flexibility; graduate student; indexing; innovation; interest; investigator training; liquid chromatography mass spectrometry; liver function; meetings; member; next generation; novel; operation; response; superfund chemical; systemic toxicity; urinary; volatile organic compound

PROJECT SUMMARY (METABOLISM AND TOXICITY CORE) The overall purpose and the function of the Metabolism and Toxicity Core is to provide high quality analytical services and validated data in support of all projects at the University of Louisville Superfund Research Center. The Core personnel will conduct measurements and assays of biomarkers and indices of cardiovascular, metabolism, and hepatic function used for assessing the risk and progression of cardiometabolic disease upon exposure to volatile organic compounds (VOCs). The Core personnel will also provide intellectual input and advice in selection of specific methodological approaches and provide training to students, fellows, technicians, and junior faculty. The Core will employ strict quality control procedures to validate data and generate harmonized data reports and data dictionaries to be stored in the Data Management and Analysis Core. The core will support all Superfund Center research projects through its state-of-the-art equipment and assay. The support offered by the core encompasses three main components – a flow cytometry component for the detection and characterization of circulating cells and microparticles; a metabolism component for measuring biochemical and metabolic endpoints such as urinary metabolites of VOCs, bile acids and catecholamines by LC/MS/MS and GC/MS; and a pathology component to assay systemic toxicity using a battery of tests relating to glucose control, liver function and hepatic toxicity. However, the scope of the core exceeds that of the projects to encompass the development of cutting-edge techniques and analytical platforms to expand its repertoire of services. The Core will continually upgrade its infrastructure as well as its technical capabilities to remain at the leading edge of assays relating to biomarkers of exposure and harm. The core is also committed to the continued education of its users as well as the research community at the university. The Core will also disseminate educational information regarding the potential utility and accessibility of its services to enhance environmental health research. By ensuring central deposition of harmonized and validated data, the core will provide a firm foundation for research not only by the University of Louisville Superfund investigators, but investigators at other Superfund Centers as well. Because of its state-of-the-art equipment, highly sophisticated technologies, and skilled technical personnel, the Core will provide premier toxicological and clinical pathology services. By meeting these goals, the core will continue to be an essential vehicle for growth and innovation within the Center, as well as an educational hub for the training the next generation of scientists in the field of environmental health research.

项目总结（代谢和毒性核心） 代谢和毒性核心的总体目的和功能是提供高质量的分析 服务和验证数据，以支持路易斯维尔大学超级基金研究中心的所有项目。 核心人员将进行心血管生物标志物和指标的测量和分析， 用于评估心脏代谢疾病的风险和进展， 暴露于挥发性有机化合物（VOC）。核心人员还将提供智力投入， 在选择具体方法方面提供咨询意见，并向学生、研究员、技术人员、 和初级教员核心将采用严格的质量控制程序来验证数据并生成 统一数据报告和数据字典，存储在数据管理和分析核心中。的 核心将支持所有超级基金中心的研究项目，通过其国家的最先进的设备和分析。的 该中心提供的支持包括三个主要组成部分： 以及循环细胞和微粒的表征;用于测量生物化学的代谢组分 和代谢终点，如通过LC/MS/MS测定的VOC、胆汁酸和儿茶酚胺的尿液代谢物， GC/MS;以及病理学部分，其使用与葡萄糖控制相关的一系列测试来测定全身毒性， 肝功能和肝毒性。然而，核心项目的范围超出了项目的范围， 开发尖端技术和分析平台，以扩大其服务范围。的 核心将不断升级其基础设施以及技术能力，以保持领先地位 与暴露和伤害的生物标志物相关的分析。核心还致力于继续教育 它的用户以及大学的研究社区。该中心还将传播教育信息， 关于其服务在加强环境卫生方面的潜在效用和可获得性的信息 research.通过确保统一和有效数据的集中存放，核心数据库将提供一个坚实的基础， 研究不仅由路易斯维尔大学超级基金的调查人员，但调查人员在其他超级基金 中心也是。由于其最先进的设备，高度复杂的技术， 技术人员，核心将提供一流的毒理学和临床病理学服务。通过满足这些 核心将继续成为中心内增长和创新的重要工具， 教育中心，培养环境健康研究领域的下一代科学家。