Skin Biology and Diseases Resource-based Center at Mount Sinai

西奈山皮肤生物学和疾病资源中心

• 批准号: 10463721
• 负责人: Elena Ezhkova
• 金额: $ 84.5万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10463721
• 关键词: ATAC-seq; Adoption; Advertising; Archives; Area; Award; Bioinformatics; Biology; Biometry; CRISPR/Cas technology; Candidate Disease Gene; Cells; ChIP-seq; Clinical; Collaborations; Communication; Communication Barriers; Communities; Complement; Consult; Consultations; Custom; Data; Data Analyses; Data Reporting; Dermatology; Development; Disease; Disease model; Educational process of instructing; Electronic Mail; Ensure; Environment; Epigenetic Process; Evaluation; Faculty; Faculty Recruitment; Financial Support; Fostering; Funding; Future; Gene Expression; Generations; Genes; Genomics; Geography; Goals; Grant; Health; Hi-C; High School Student; Hospitals; Human; Individual; Infrastructure; Institution; Interdisciplinary Communication; Internships; Junior Physician; Laboratories; Leadership; Mentors; Minority; Modeling; Molecular; Mus; NCI Scholars Program; National Institute of Arthritis and Musculoskeletal and Skin Diseases; New York; Patients; Persons; Physicians; Population Heterogeneity; Positioning Attribute; Research; Research Personnel; Resources; Sampling; Scholarship; Scientist; Services; Skin; Small RNA; Standardization; Structure; Study models; Technology; Testing; Therapeutic; Training; Training Support; Translations; Universities; analysis pipeline; base; combat; cost; data integration; data visualization; design; exome sequencing; genetic analysis; genome sequencing; human disease; improved; induced pluripotent stem cell; innovation; laboratory experience; member; minority children; minority scientist; minority student; mouse model; novel; outreach; programs; racial disparity; recruit; screening; single cell analysis; single-cell RNA sequencing; skin barrier; skin disorder; skin organogenesis; social media; success; transcriptome sequencing; transcriptomics; underserved minority; user-friendly; virtual; web site; whole genome

Summary - Overall The overall goal of the Skin Biology Resource-Based Center (SBDRC) at Mount Sinai is to accelerate research in skin biology and diseases and promote its translation to benefit human health. To achieve this, we will harness the technical innovations, high-end infrastructure and computing power available at Mount Sinai to specifically advance and innovate skin research. In parallel we will ensure a vibrant future for skin biology and diseases research by expanding our research community and increasing its diversity. Administrative Core A will provide management, structure and oversight; award User Scholarships to reduce cost barriers to high end technologies; communicate SBDRC programs through a website and social media; organize research talks, seminars and retreats to promote scientific interchange and collaborations; attract new investigators and support development of junior and physician-scientists through Pilot Grants and Individual Mentoring Committees; and improve diversity through Mount Sinai's newly established Laureates Program that facilitates targeted faculty recruitment, and by establishing the SBDRC Sinai Skin Scholars Program, which will introduce underserved East Harlem high school students to skin biology and diseases research. Our Resource Cores are designed to capitalize on Mount Sinai's institutionally-supported and highly innovative technological infrastructure and expertise. We will support effort for technological experts to prioritize SBDRC goals and immerse themselves in skin research labs. Each Resource Core will provide unique expertise from leaders in diverse research areas, and technology, training and services customized to users' needs. The 3 Resource Cores will interact seamlessly: Resource Core B, “Modeling of Skin Disease for Mechanistic Analysis and Therapeutic Discovery,” will facilitate access to human skin samples, and provide technology, consultation and assistance with multiplexed immunostaining, isolation of skin cell subpopulations, generation and analysis of genetic mouse models, and gene editing and gene expression manipulation in human iPSC-derived skin cells. Skin cells or samples from Core B will be analyzed at the molecular level in Resource Core C, “Skin Genomics, Transcriptomics, and Epigenetics Core,” which will provide access to genomics (exome sequencing, whole genome sequencing, and targeted capture), transcriptomics (RNA-seq, ISO-seq, small RNA seq), epigenetic (ATAC-seq, ChIP-seq, CUT&RUN, Hi-C), and single-cell (scRNA-seq, scATAC-seq, spatial transcriptomics) technologies. Resource Core D, “Data Analysis and Integration Core,” will provide standardized biostatistical and bioinformatics analysis and integration of Core C data and make these easily available through Skin-GLOW, a novel user-friendly interface for data visualization. In turn, data analyzed in Core D will yield candidate genes and mechanisms that will be tested through functional analyses provided in Core B. Our plans will create a productive, impactful and extensive skin Research Community including strong external hubs at NYU and at Yale University.

摘要--总体 西奈山皮肤生物学资源中心(SBDRC)的总体目标是加速研究 在皮肤生物学和疾病方面，并促进其翻译，以造福人类健康。为了实现这一目标，我们将 利用西奈山的技术创新、高端基础设施和计算能力 具体推进和创新皮肤研究。同时，我们将确保皮肤生物学和 通过扩大我们的研究社区和增加其多样性来进行疾病研究。管理核心A 将提供管理、结构和监督；奖励用户奖学金以降低进入高端市场的成本壁垒 技术；通过网站和社交媒体交流SBDRC计划；组织研究讲座， 研讨会和务虚会，以促进科学交流和合作；吸引新的研究人员和 通过试点资助和个人指导支持初级和内科科学家的发展 委员会；并通过西奈山新设立的桂冠计划促进多样性 有针对性地招聘教师，并建立SBDRC西奈皮肤学者计划，该计划将引入 服务不足的东哈莱姆高中生从事皮肤生物学和疾病研究。我们的资源核心 旨在利用西奈山得到制度支持和高度创新的技术 基础设施和专业知识。我们将支持技术专家优先考虑SBDRC目标和 沉浸在皮肤研究实验室中。每个资源核心将提供来自以下领域领导者的独特专业知识 不同的研究领域，以及根据用户需求定制的技术、培训和服务。三大资源 核心将进行无缝交互：资源核心B，“用于机械分析的皮肤病建模和 治疗性发现“将促进人类皮肤样本的获取，并提供技术、咨询 并协助多重免疫染色、皮肤细胞亚群的分离、生成和分析 小鼠遗传模型，以及人iPSC来源皮肤的基因编辑和基因表达操作 细胞。来自核心B的皮肤细胞或样本将在资源核心C中的分子水平上进行分析 基因组学、转录学和表观遗传学核心，这将提供对基因组学(外显子组)的访问 测序、全基因组测序和靶向捕获)、转录学(RNA-seq、ISO-seq、Small RNA SEQ)、表观遗传(ATAC-SEQ、CHIP-SEQ、CUT&RUN、Hi-C)和单细胞(scRNA-SEQ、scATAC-SEQ、 空间转录学)技术。资源核心D，“数据分析和集成核心”，将提供 标准化的生物统计和生物信息学分析以及核心C数据的集成，使这些 可通过Skin-Glow提供，这是一种用于数据可视化的新型用户友好界面。反过来，分析的数据 核心D将产生候选基因和机制，将通过 核心B.我们的计划将创建一个高效、有影响力和广泛的皮肤研究社区，包括Strong 纽约大学和耶鲁大学的外部中心。