Skin Biology and Diseases Resource-based Center at Mount Sinai

总结 - 总体 西奈山皮肤生物学资源中心 (SBDRC) 的总体目标是加速研究 皮肤生物学和疾病领域的研究并促进其转化以造福人类健康。为了实现这一目标，我们将 利用西奈山现有的技术创新、高端基础设施和计算能力 专门推进和创新皮肤研究。与此同时，我们将确保皮肤生物学和 通过扩大我们的研究群体并增加其多样性来进行疾病研究。行政核心A 将提供管理、结构和监督；授予用户奖学金以减少进入高端市场的成本障碍 技术；通过网站和社交媒体传达 SBDRC 计划；组织研究讲座， 举办研讨会和务虚会，以促进科学交流与合作；吸引新的调查人员并 通过试点拨款和个人指导支持初级科学家和医师科学家的发展 委员会；并通过西奈山新设立的获奖者计划提高多样性，该计划促进 有针对性的教师招聘，并建立 SBDRC 西奈皮肤学者计划，该计划将介绍 东哈莱姆区高中生的皮肤生物学和疾病研究服务不足。我们的资源核心 旨在利用西奈山的机构支持和高度创新的技术 基础设施和专业知识。我们将支持技术专家优先考虑 SBDRC 目标和 沉浸在皮肤研究实验室中。每个资源核心都将提供来自以下领域领导者的独特专业知识 多元化的研究领域，以及根据用户需求定制的技术、培训和服务。 3 资源 核心将无缝交互：资源核心 B，“皮肤病建模以进行机械分析和 治疗发现”将促进获取人体皮肤样本，并提供技术、咨询 并协助多重免疫染色、皮肤细胞亚群的分离、生成和分析 基因小鼠模型的研究，以及人类 iPSC 衍生皮肤的基因编辑和基因表达操作 细胞。来自核心 B 的皮肤细胞或样本将在资源核心 C“皮肤”中进行分子水平分析 基因组学、转录组学和表观遗传学核心”，这将提供基因组学（外显子组） 测序、全基因组测序和靶向捕获）、转录组学（RNA-seq、ISO-seq、小 RNA seq）、表观遗传学（ATAC-seq、ChIP-seq、CUT&RUN、Hi-C）和单细胞（scRNA-seq、scATAC-seq、 空间转录组学）技术。资源核心 D，“数据分析和集成核心”，将提供 标准化生物统计和生物信息学分析以及 Core C 数据的集成，使这些变得容易 可通过 Skin-GLOW 获得，这是一种新颖的用户友好界面，用于数据可视化。反过来，数据分析 Core D 将产生候选基因和机制，并通过中提供的功能分析进行测试。 核心 B. 我们的计划将创建一个富有成效、有影响力和广泛的皮肤研究社区，包括强大的 纽约大学和耶鲁大学的外部中心。

Summary - Overall The overall goal of the Skin Biology Resource-Based Center (SBDRC) at Mount Sinai is to accelerate research in skin biology and diseases and promote its translation to benefit human health. To achieve this, we will harness the technical innovations, high-end infrastructure and computing power available at Mount Sinai to specifically advance and innovate skin research. In parallel we will ensure a vibrant future for skin biology and diseases research by expanding our research community and increasing its diversity. Administrative Core A will provide management, structure and oversight; award User Scholarships to reduce cost barriers to high end technologies; communicate SBDRC programs through a website and social media; organize research talks, seminars and retreats to promote scientific interchange and collaborations; attract new investigators and support development of junior and physician-scientists through Pilot Grants and Individual Mentoring Committees; and improve diversity through Mount Sinai's newly established Laureates Program that facilitates targeted faculty recruitment, and by establishing the SBDRC Sinai Skin Scholars Program, which will introduce underserved East Harlem high school students to skin biology and diseases research. Our Resource Cores are designed to capitalize on Mount Sinai's institutionally-supported and highly innovative technological infrastructure and expertise. We will support effort for technological experts to prioritize SBDRC goals and immerse themselves in skin research labs. Each Resource Core will provide unique expertise from leaders in diverse research areas, and technology, training and services customized to users' needs. The 3 Resource Cores will interact seamlessly: Resource Core B, “Modeling of Skin Disease for Mechanistic Analysis and Therapeutic Discovery,” will facilitate access to human skin samples, and provide technology, consultation and assistance with multiplexed immunostaining, isolation of skin cell subpopulations, generation and analysis of genetic mouse models, and gene editing and gene expression manipulation in human iPSC-derived skin cells. Skin cells or samples from Core B will be analyzed at the molecular level in Resource Core C, “Skin Genomics, Transcriptomics, and Epigenetics Core,” which will provide access to genomics (exome sequencing, whole genome sequencing, and targeted capture), transcriptomics (RNA-seq, ISO-seq, small RNA seq), epigenetic (ATAC-seq, ChIP-seq, CUT&RUN, Hi-C), and single-cell (scRNA-seq, scATAC-seq, spatial transcriptomics) technologies. Resource Core D, “Data Analysis and Integration Core,” will provide standardized biostatistical and bioinformatics analysis and integration of Core C data and make these easily available through Skin-GLOW, a novel user-friendly interface for data visualization. In turn, data analyzed in Core D will yield candidate genes and mechanisms that will be tested through functional analyses provided in Core B. Our plans will create a productive, impactful and extensive skin Research Community including strong external hubs at NYU and at Yale University.