Full Field OCT for Cellular Level Structural and Functional Retinal Imaging

用于细胞水平结构和功能性视网膜成像的全视场 OCT

• 批准号: 10470228
• 负责人: Nathan Doble
• 金额: $ 54.9万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10470228
• 关键词: Address; Age; Age related macular degeneration; Anesthetics; Animal Model; Animals; Austria; Back; Brain; Cells; Clinical; Collaborations; Cone; Dark Adaptation; Data; Development; Devices; Disease; Drusen; Electroretinography; Exhibits; Eye; Four-dimensional; Fundus photography; Future; Generations; Genes; Human; Image; Imaging technology; Individual; Kinetics; Lateral; Length; Light; Link; Maps; Measurement; Measures; Medical; Methods; Microscopic; Modality; Modeling; Mus; Neurons; Nonexudative age-related macular degeneration; Ohio; Optical Coherence Tomography; Optics; Outcome; Performance; Perimetry; Phase; Physiological; Physiology; Positioning Attribute; Protocols documentation; Publishing; Reporting; Reproducibility; Resolution; Retina; Retinal Cone; Retinal Degeneration; Retinal Diseases; Sampling; Scanning; Scheme; Signal Transduction; Source; Speed; Stimulus; Structure of retinal pigment epithelium; System; Techniques; Testing; Time; Transgenic Mice; Universities; Validation; Vertebrate Photoreceptors; Vision; Visual Fields; Visualization; Work; adaptive optics; analog; awake; base; clinical translation; cohort; comparative; computerized tools; cost; design; design and construction; design-build-test; digital; electric field; experimental study; human imaging; human model; human subject; imaging system; in vivo; in vivo imaging; instrumentation; light scattering; millisecond; mouse model; nanometer; normal aging; novel; novel therapeutics; response; retinal imaging; retinal rods; stem cell therapy; temporal measurement; tool

Project Summary The proposed design and construction of an optical coherence tomography (OCT) system, namely, a full-field (FF)-swept-source (SS)-OCT will allow rapid structural and functional measures of individual cone and rod photoreceptors (PRCs), the sub-retinal space (SRS) and retinal pigment epithelial (RPE) cells to an external light stimulus. Current OCT systems either have limited temporal resolution i.e. they are not fast enough to measure the neuronal response or lack the spatial resolution to resolve individual cells. Here, we will exploit the extremely high parallel image acquisition speed of FF-SS-OCT to study neuronal responses as short as a few milliseconds. Furthermore, as the system collects the whole back scattered electric field from the sample, numerical aberration correction (NAC) methods can be used allowing for the visualization of single cells without the need for techniques such as hardware based adaptive optics (AO). There are three stages to the proposed project: (i) the design and construction of FF-SS-OCT systems for both human and animal models (mice) of retinal disease - examining both species in parallel will speed up the clinical translation, (ii) testing the system performance in healthy retina of both humans and mice, (iii) measure the sensitivity of the system to detect microstructural functional changes by comparing age-matched controls to diseased cohorts. These will be early stage dry age-related macular degeneration (AMD) subjects and several AMD mouse models. Scientific rigor and reproducibility will be addressed by comparing FF-SS-OCT structural images to those obtained with our existing human and mice AO-OCT systems; functional measurements in human subjects will be compared to published data and also to clinical measures such as mfERG and visual fields. Functional measurements in mice will be compared to published data and also to the results from our first-generation mouse functional retinal imaging system and Ganzfeld ERG. Many potential therapies are under development for a range of ocular diseases, these systems fulfill a critical need for modalities that can not only determine whether the neurons are structurally intact but importantly are also exhibiting normal function.

项目概要 光学相干断层扫描（OCT）系统的拟议设计和构造，即全视场 (FF)-扫描源 (SS)-OCT 将允许对单个锥体和杆体进行快速结构和功能测量 光感受器 (PRC)、视网膜下腔 (SRS) 和视网膜色素上皮 (RPE) 细胞对外部光线的感知 刺激。当前的 OCT 系统要么时间分辨率有限，即测量速度不够快 神经元反应或缺乏解析单个细胞的空间分辨率。在这里，我们将利用极端 FF-SS-OCT 的高并行图像采集速度可研究短至几毫秒的神经元反应。 此外，当系统从样品收集整个背向散射电场时，数值像差 可以使用校正（NAC）方法来实现单个细胞的可视化，而无需 基于硬件的自适应光学 (AO) 等技术。 拟议项目分为三个阶段：(i) FF-SS-OCT 系统的设计和建造 视网膜疾病的人类和动物模型（小鼠） - 并行检查这两个物种将加快临床速度 翻译，(ii) 测试人类和小鼠健康视网膜中的系统性能，(iii) 测量 通过比较年龄匹配的对照，系统检测微观结构功能变化的灵敏度 患病群体。这些将是早期干性年龄相关性黄斑变性（AMD）受试者和一些 AMD 鼠标型号。 通过将 FF-SS-OCT 结构图像与那些图像进行比较，可以解决科学严谨性和可重复性问题。 使用我们现有的人类和小鼠 AO-OCT 系统获得；人类受试者的功能测量将 与已发表的数据以及 mfERG 和视野等临床测量结果进行比较。功能性 小鼠测量结果将与已发表的数据以及我们第一代小鼠的结果进行比较 功能性视网膜成像系统和 Ganzfeld ERG。 针对一系列眼部疾病的许多潜在疗法正在开发中，这些系统满足了关键的要求 需要不仅可以确定神经元结构是否完整，而且重要的是可以确定的模式 也表现出正常的功能。