In-vivo, High Contrast Imaging of the Human Rod Photoreceptor Mosaic

人杆光感受器马赛克的体内高对比度成像

• 批准号: 8326718
• 负责人: Nathan Doble
• 金额: $ 30万
• 依托单位: NEW ENGLAND COLLEGE OF OPTOMETRY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8326718
• 关键词: Affect; Age related macular degeneration; Animal Model; Blindness; Cell Density; Cells; Clinical; Clinical Trials; Complement; Dark Adaptation; Data; Development; Disease; Eye; Fundus; Goals; Human; Image; Imagery; Imaging technology; Individual; Lead; Leukocytes; Life; Masks; Measures; New England; Optics; Optometry; Outcome Measure; Patients; Performance; Pharmaceutical Preparations; Pharmacotherapy; Process; Property; Reporting; Resolution; Retina; Retinal; Retinal Cone; Retinal Diseases; Retinitis Pigmentosa; Signal Transduction; Simulate; Structure; Structure of retinal pigment epithelium; System; Techniques; Testing; Time; Work; adaptive optics; arm; cell type; college; density; disease diagnosis; improved; in vivo; optical imaging; receptor; receptor coupling; retinal rods; success; tool

Project Summary The goal is to enhance the resolution and contrast of in-vivo images of the rod photoreceptor mosaic taken with high resolution adaptive optics (AO) equipped retinal cameras. There has been great success in imaging other cell types such as the cones, retinal pigment epithelium and leukocytes cells but to date nobody has reported in-vivo evidence for the rods. The project will examine advanced optical techniques that can be combined with AO to further improve the contrast of the retinal images at the spatial scale of the rods and foveal cones. These may be advanced masks to suppress unwanted structure in the images complemented with approaches such as using the Stiles- Crawford effect and deconvolution. Achieving this goal will allow one, for example, to count individual rod cells and calculate cell densities which can then be compared to histological measures. Initially, young, normal subjects will be imaged to refine and optimize the new imaging approaches. As the work progresses, the study will examine diseases that target the rod cells such as retinitis pigmentosa and age related macular degeneration and relate the changes observed to standard clinical tests. This work will lead to better understanding of retinal structure in both normal and diseased eyes. With new drug treatments being developed in animal models, the direct non-invasive imaging of rod cells would provide an important outcome measure when these therapies are transitioned into human clinical trials.

项目摘要 我们的目标是提高分辨率和对比度的活体图像的视杆细胞马赛克采取 配备高分辨率自适应光学（AO）视网膜相机。在成像方面取得了巨大的成功 其他细胞类型，如视锥细胞、视网膜色素上皮细胞和白细胞，但迄今为止还没有人 报告了棒的体内证据。 该项目将研究先进的光学技术，可以与AO相结合，以进一步提高 视网膜图像在视杆和中央凹视锥的空间尺度上的对比度。这些可能是高级面具 为了抑制图像中不需要的结构，辅以诸如使用Stiles的方法， 克劳福德效应与反卷积。实现这一目标将允许一个，例如，计数个别杆细胞 并计算细胞密度，然后将其与组织学测量进行比较。 最初，将对年轻的正常受试者进行成像，以改进和优化新的成像方法。为 随着工作的进展，这项研究将检查针对视杆细胞的疾病，如视网膜色素变性和年龄 相关的黄斑变性，并将观察到的变化与标准临床测试相关联。 这项工作将导致更好地了解视网膜结构在正常和患病的眼睛。用新 在动物模型中开发的药物治疗，视杆细胞的直接非侵入性成像将提供 当这些疗法过渡到人体临床试验时，这是一个重要的结果指标。