Full Field OCT for Cellular Level Structural and Functional Retinal Imaging

用于细胞水平结构和功能性视网膜成像的全视场 OCT

• 批准号: 10052709
• 负责人: Nathan Doble
• 金额: $ 57.4万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10052709
• 关键词: Address; Age; Age related macular degeneration; Anesthetics; Animal Model; Animals; Austria; Back; Brain; Cells; Clinical; Collaborations; Cone; Dark Adaptation; Data; Development; Devices; Disease; Drusen; Electroretinography; Exhibits; Eye; Four-dimensional; Fundus photography; Future; Generations; Genes; Human; Image; Imaging technology; Individual; Kinetics; Lateral; Length; Light; Link; Maps; Measurement; Measures; Medical; Methods; Microscopic; Modality; Modeling; Mus; Neurons; Nonexudative age-related macular degeneration; Ohio; Optical Coherence Tomography; Optics; Outcome; Performance; Perimetry; Phase; Physiological; Physiology; Positioning Attribute; Protocols documentation; Publishing; Reporting; Reproducibility; Resolution; Retina; Retinal Cone; Retinal Degeneration; Retinal Diseases; Sampling; Scanning; Scheme; Signal Transduction; Source; Speed; Stimulus; Structure; Structure of retinal pigment epithelium; System; Techniques; Testing; Time; Transgenic Mice; Universities; Validation; Vertebrate Photoreceptors; Vision; Visual Fields; Visualization; Work; adaptive optics; analog; awake; base; clinical translation; cohort; comparative; computerized tools; cost; design; design and construction; digital; electric field; experimental study; human imaging; human model; human subject; imaging system; in vivo; in vivo imaging; instrumentation; light scattering; millisecond; mouse model; nanometer; normal aging; novel; novel therapeutics; response; retinal imaging; retinal rods; stem cell therapy; temporal measurement; tool

Project Summary The proposed design and construction of an optical coherence tomography (OCT) system, namely, a full-field (FF)-swept-source (SS)-OCT will allow rapid structural and functional measures of individual cone and rod photoreceptors (PRCs), the sub-retinal space (SRS) and retinal pigment epithelial (RPE) cells to an external light stimulus. Current OCT systems either have limited temporal resolution i.e. they are not fast enough to measure the neuronal response or lack the spatial resolution to resolve individual cells. Here, we will exploit the extremely high parallel image acquisition speed of FF-SS-OCT to study neuronal responses as short as a few milliseconds. Furthermore, as the system collects the whole back scattered electric field from the sample, numerical aberration correction (NAC) methods can be used allowing for the visualization of single cells without the need for techniques such as hardware based adaptive optics (AO). There are three stages to the proposed project: (i) the design and construction of FF-SS-OCT systems for both human and animal models (mice) of retinal disease - examining both species in parallel will speed up the clinical translation, (ii) testing the system performance in healthy retina of both humans and mice, (iii) measure the sensitivity of the system to detect microstructural functional changes by comparing age-matched controls to diseased cohorts. These will be early stage dry age-related macular degeneration (AMD) subjects and several AMD mouse models. Scientific rigor and reproducibility will be addressed by comparing FF-SS-OCT structural images to those obtained with our existing human and mice AO-OCT systems; functional measurements in human subjects will be compared to published data and also to clinical measures such as mfERG and visual fields. Functional measurements in mice will be compared to published data and also to the results from our first-generation mouse functional retinal imaging system and Ganzfeld ERG. Many potential therapies are under development for a range of ocular diseases, these systems fulfill a critical need for modalities that can not only determine whether the neurons are structurally intact but importantly are also exhibiting normal function.

项目摘要 提出了一种光学相干层析成像(OCT)系统，即全场系统的设计和构造 (FF)-扫掠震源(SS)-OCT将允许对单个锥体和杆进行快速结构和功能测量 光感受器(PRCs)、视网膜下间隙(SRS)和视网膜色素上皮(RPE)细胞对外部光线的反应 刺激。当前的OCT系统要么具有有限的时间分辨率，即它们的速度不足以测量 神经元的反应或缺乏空间分辨率来分解单个细胞。在这里，我们将极大地利用 FF-SS-OCT的高并行图像采集速度，用于研究短至几毫秒的神经元反应。 此外，当系统从样品中收集整个背向散射电场时，数值像差 可以使用校正(NAC)方法来实现单个细胞的可视化，而不需要 基于硬件的自适应光学(AO)等技术。 拟议项目分为三个阶段：(I)设计和建造两个系统的FF-SS-OCT系统 人类和动物视网膜疾病模型(鼠)-并行检查这两个物种将加快临床 翻译，(Ii)测试系统在人类和小鼠的健康视网膜中的性能，(Iii)测量 通过比较年龄匹配的对照组和对照组检测微结构功能变化的系统敏感性 患病的人群。这些将是早期干性老年性黄斑变性(AMD)的受试者和几个 AMD小鼠模型。 科学的严谨性和重复性将通过比较FF-SS-OCT结构图像和 通过我们现有的人类和小鼠AO-OCT系统获得；在人类受试者中的功能测量将 与已发表的数据以及临床测量(如mfERG和视野)进行比较。功能性 在老鼠身上的测量结果将与公布的数据以及我们第一代老鼠的结果进行比较 功能性视网膜成像系统和Ganzfeld视网膜电流图。 许多潜在的治疗方法正在开发中，用于治疗一系列眼部疾病，这些系统实现了关键的 需要一种不仅可以确定神经元结构是否完整的模式，而且重要的是 也表现出正常的功能。