Optimization, application and dissemination of high-speed hybrid multiphoton volumetric imaging technologies

高速混合多光子体积成像技术的优化、应用和推广

基本信息

  • 批准号:
    10471831
  • 负责人:
  • 金额:
    $ 95.45万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-09-01 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY / ABSTRACT Understanding how cognitively-relevant behavioral functions emerge from activity patterns of identified cell- types is predicated on the ability to record large-scale ensemble dynamics from genetically-identified and longitudinally-tracked neuronal populations across multiple brain regions and layers with high spatial and temporal resolution over behaviorally-relevant time-scales. Two-photon scanning microscopy in combination with genetically-encoded calcium (Ca2+) indicators is currently the most essential tool for in vivo optical recording of neuronal activity, its application to deep brain regions. However, currently the commercially available 2pM systems are limited in their applications due to constraints related to the obtainable imaging depth, volumetric field-of-view (VFOV), and temporal resolution at which neuronal population dynamics can be effectively captured. We have recently developed and demonstrated the proof of principle of a new high-speed volumetric Ca2+-imaging platform termed Hybrid Multiplexed Sculpted Light (HyMS) Microscopy that combines 2pM with three-photon microscopy (3pM). HyMS allows for volumetric recording of neuroactivity at single-cell resolution within volumes up to ~1 × 1 × 1.22 mm at up to 17 Hz in cortical as well as sub-cortical regions of awake behaving mice. The impact of this tool will depend on a successful optimization, neurobiological application and dissemination strategy within the neuroscience community. While we will provide open source access for technically skilled labs, given the technical complexity and costs of such a system, the most effective strategy is through partnership with industry and through commercialization of the system. Here we propose a roadmap towards this objective. Building on our current existing system, we will implement a number of technical refinements and optimizations. Leveraging the ongoing collaboration with the Losonczy Lab at the Columbia University, we will use our optimized HyMS system to perform high-speed multiphoton volumetric Ca2+ imaging of functional circuitry across the entire depth of the mouse dorsal hippocampus (HPC), encompassing all major regions of the HPC trisynaptic circuitry. This application will provide us valuable feedback for further optimization and refinement and development of our HyMS prototype system. In parallel, we will develop together with our industrial partner a first prototype of the HyMS system (-HyMS) This prototype will be again used and tested by the Losonczy Lab. The obtained insights and user feedback from their application will drive the development of a beta prototype (-HyMS) which will be used to engage broader local users as beta testers. 9 user labs, mainly from the NYC area, with a broad range of biological questions and applications, will participate as beta testers and provide us with iterative user feedback which will ultimately drive and be incorporated both into the into the commercialization of HyMS as well its open source model of the access to this technology.
项目摘要/摘要

项目成果

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Attila Losonczy其他文献

Attila Losonczy的其他文献

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{{ truncateString('Attila Losonczy', 18)}}的其他基金

Activity-dependent endocannabinoid control in epilepsy
癫痫的活动依赖性内源性大麻素控制
  • 批准号:
    10639147
  • 财政年份:
    2023
  • 资助金额:
    $ 95.45万
  • 项目类别:
2023 NINDS Landis Mentorship Award - Administrative Supplement to NS121106 Control of Axon Initial Segment in Epilepsy
2023 年 NINDS 兰迪斯指导奖 - NS121106 癫痫轴突初始段控制的行政补充
  • 批准号:
    10896844
  • 财政年份:
    2023
  • 资助金额:
    $ 95.45万
  • 项目类别:
Local Circuit Control of Rapid Plasticity and Tunable Ensemble Formation in the Hippocampus
海马体快速可塑性和可调系综形成的局部电路控制
  • 批准号:
    10725714
  • 财政年份:
    2023
  • 资助金额:
    $ 95.45万
  • 项目类别:
Control of Axon Initial Segment in Epilepsy
癫痫轴突起始段的控制
  • 批准号:
    10383771
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Experimental and modeling investigations into microcircuit, cellular and subcellular determinants of hippocampal ensemble recruitment to contextual representations
对海马体集合招募到情境表征的微电路、细胞和亚细胞决定因素的实验和建模研究
  • 批准号:
    10535439
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Experimental and modeling investigations into microcircuit, cellular and subcellular determinants of hippocampal ensemble recruitment to contextual representations
对海马体集合招募到情境表征的微电路、细胞和亚细胞决定因素的实验和建模研究
  • 批准号:
    10321652
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Control of Axon Initial Segment in Epilepsy
癫痫轴突起始段的控制
  • 批准号:
    10600120
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Experimental and modeling investigations into microcircuit, cellular and subcellular determinants of hippocampal ensemble recruitment to contextual representations
对海马体集合招募到情境表征的微电路、细胞和亚细胞决定因素的实验和建模研究
  • 批准号:
    10097137
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Control of Axon Initial Segment in Epilepsy
癫痫轴突起始段的控制
  • 批准号:
    10183360
  • 财政年份:
    2021
  • 资助金额:
    $ 95.45万
  • 项目类别:
Optimization, application and dissemination of high-speed hybrid multiphoton volumetric imaging technologies
高速混合多光子体积成像技术的优化、应用和推广
  • 批准号:
    10681436
  • 财政年份:
    2020
  • 资助金额:
    $ 95.45万
  • 项目类别:

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