Enhancing NARMS surveillance of antibiotic resistance in retail foods in Hawaii

项目总结/摘要 抗生素耐药性（AMR）是食品安全和公共卫生的重要问题。的 国家抗菌素耐药性监测系统（NARMS）零售食品监测 夏威夷从2020年开始。该项目将继续NARMS零售食品监测， 人口代表夏威夷州的城市和农村地区。该项目的目标是 改善食物安全和保障公众健康， 具体目标和活动。首先，我们将继续NARMS零售食品 在夏威夷州进行监视。从2021年开始，我们的抽样将覆盖城市和农村 国家的地区。其次，我们将监测零售肉类中不同病原体的AMR趋势 和海鲜标本。我们将收集零售肉类（鸡肉，火鸡，猪肉，牛肉）和海鲜 （虾，鲑鱼，罗非鱼）标本每月从随机选择的杂货店在选定的 城市和农村地区。我们将测试肉类样本的沙门氏菌，弯曲杆菌（仅家禽），大肠杆菌。 大肠杆菌和肠球菌以及海产品样品中的肠球菌、气单胞菌、弧菌等 根据NARMS的零售食品协议，乳糖阳性细菌。细菌分离物将 每月发送给FDA进行抗菌药物敏感性测试（AST）和全基因组测序 (WGS)（肉类分离物的WGS将由UC Davis完成）。第三，我们将开展 零售食品中抗生素耐药性流行病学研究。我们将收集店铺信息 和每个零售肉类和海鲜标本的元数据，并确定 细菌及其AMR（例如，单一或多重耐药性）和菌种的流行率， 零售食品的来源、类型和形式、抗生素使用的声明和季节等。第四， 根据这个项目的结果，我们将编写非专业出版物、外展材料、摘要和 海报和同行评审的出版物。我们将把研究结果传播给农业， 水产养殖社区，兽医和公众通过通讯，推广讲习班， 在各种会议和同行评审期刊上发表演讲。第五，我们将参与 在NARMS电话会议和工作组中。 通过实现这些具体目标和开展活动，该项目将反映以下趋势： 夏威夷州零售食品中的AMR。该项目将加强NARMS零售食品 监测和提高公众对零售食品中抗生素耐药性的认识。该项目还将 加强联邦和州机构与学术界在研究、检测 监测和调查与零售食品中抗生素耐药性相关的食源性疫情。 1

Project Summary/Abstract Antimicrobial resistance (AMR) is an important issue for food safety and public health. The National Antimicrobial Resistance Monitoring System (NARMS) Retail Food Surveillance in Hawaii started in 2020. This project will continue the NARMS retail food surveillance in population representative urban and rural areas in the state of Hawaii. The project’s goal of improving food safety and protecting public health will be achieved by undertaking the following specific aims and activities. First, we will continue the NARMS retail food surveillance in the state of Hawaii. Starting from 2021, our sampling will cover city and rural areas of the state. Second, we will monitor trends of AMR in different pathogens in retail meat and seafood specimens. We will collect retail meat (chicken, turkey, pork, beef) and seafood (shrimp, salmon, tilapia) specimens monthly from randomly selected grocery stores in selected city and rural areas. We will test meat samples for Salmonella, Campylobacter (poultry only), E. coli and Enterococcus and seafood samples for Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria according to NARMS’ Retail Food Protocols. Bacterial isolates will be sent to FDA monthly for antimicrobial susceptibility test (AST) and whole genome sequencing (WGS) (WGS of meat isolates will be done by UC Davis). Third, we will conduct epidemiological studies on prevalence of AMR in retail food. We will collect store information and metadata for each retail meat and seafood specimen and determine key links between the prevalence of bacteria and their AMR (e.g. single or multiple drug resistance) and species, origins, types, and forms of retail food, claims of antibiotics use, and seasons etc. Fourth, based on results of this project, we will develop lay publications, outreach materials, abstracts and posters, and peer-reviewed publications. We will disseminate findings to agriculture and aquaculture communities, veterinarians, and the public via newsletters, extension workshops, presentations in meetings and conferences, and peer-reviewed journals. Fifth, we will participate in NARMS conference calls and working groups. By accomplishing these specific aims and activities, the project will characterize the trends of AMR in retail foods in the state of Hawaii. The project will enhance NARMS Retail Food Surveillance and increase public awareness of AMR in retail foods. The project will also strengthen collaborations among federal and state agencies and academia on research, detection, surveillance, and investigation of foodborne outbreaks associated with AMR in retail food. 1