NARMS surveillance of antibiotic resistance in retail meat in southern California

项目总结/摘要 目前，几乎没有关于从细菌中分离出的细菌的抗生素耐药性水平的数据。 在南加州大都市的零售店出售的生肉。为了增强 国家抗菌药物耐药性监测的抗生素耐药性监测数据 系统（NARMS），西部食品安全和安全研究所（WIFSS）和学校 加州大学戴维斯分校的兽医学（SVM）提议与FDA合作调查抗生素 从大洛杉矶地区（LA）零售肉类中分离出的细菌的耐药性。这 将通过实现三个具体目标来实现这一目标。一、零售鸡、地 火鸡，碎牛肉和猪排将每月从零售店购买， 具有不同人口统计的地理区域（东洛杉矶，安大略，西洛杉矶， Irvine）。肉类样品将在冷藏条件下运输到加州大学戴维斯分校WIFSS实验室 其中沙门氏菌、弯曲杆菌、E.大肠杆菌和肠球菌将进行 根据NARMS零售肉类监督实验室协议。确认的分离株 沙门氏菌、弯曲杆菌、E.大肠杆菌和肠球菌将被运送到FDA中心， 兽医学（CVM）月刊。其次，我们将建立一个细菌抗生素数据库， 零售肉类的耐药性，以更好地将我们的结果与农场抗生素耐药性监测联系起来 作为加州促进谨慎使用抗生素努力的一部分， 减少农场层面的抗生素耐药性。该数据库将包括这四种疾病的患病率 每种肉类的细菌种类，沙门氏菌的血清型，细菌抗生素的模式 抗性和样品信息（零售地点和邮政编码，肉类的来源和品牌， 处理器、包装等）。将这一新数据库与国家档案管理处现有的数据库连接起来， 扩大NARMS的肉类监测，促进关于以下方面的高维分析： 追踪从农场到零售店的食源性病原体和抗生素耐药性。我们的第三 目的是加强联邦、州和学术界利益相关者之间的合作， 食源性疾病监测和疫情调查。本项目的合作伙伴 包括FDA-CVM、加州食品和农业部（CDFA）、加州 公共卫生部（CDPH），加州大学戴维斯分校WIFSS和SVM。通过实现这些 NARMS系统将进一步扩大对抗生素耐药性的监测， 零售肉类中的细菌，并促进联邦和州机构和学术界之间的合作 在检测、监测和调查与抗生素有关食源性疫情中， 阻力

Project Summary/Abstract Currently there is little data regarding levels of antibiotic resistance for bacteria isolated from raw meat sold in retail stores from metropolitan Southern California. In order to enhance the antibiotic resistance surveillance data for the National Antimicrobial Resistance Monitoring System (NARMS), the Western Institute for Food Safety and Security (WIFSS) and School of Veterinary Medicine (SVM) at UC Davis propose a collaboration with FDA to survey antibiotic resistance of bacteria isolated from retail meat in the Greater Los Angeles Area (LA). This objective will be achieved by undertaking three specific aims. First, retail chicken, ground turkey, ground beef, and pork chops will be purchased monthly from retail stores in four geographical regions with different population demographics (East LA, Ontario, West LA, Irvine). Meat samples will be transported under refrigeration to the UC Davis WIFSS laboratory where isolation of Salmonella, Campylobacter, E. coli and Enterococcus will be conducted according to NARMS Retail Meat Surveillance Laboratory Protocol. Confirmed isolates of Salmonella, Campylobacter, E. coli and Enterococcus will be shipped to FDA-Center for Veterinary Medicine (CVM) monthly. Second, we will create a database of bacterial antibiotic resistance in retail meat to better link our results to farm antibiotic resistance surveillance required by Senate Bill-27 as part of California’s effort to promote prudent antibiotic use and reduce antibiotic resistance at the farm level. The database will include prevalence of the four bacteria species for each type of meat, serotypes of Salmonella, patterns of bacterial antibiotic resistance and sample information (retail location and zip code, source and brand of meat, processor, packaging, etc.). Linking this new database to NARMS’s existing databases will expand NARMS’ meat surveillance and facilitate high-dimensional analysis with respect to tracing foodborne pathogens and antibiotic resistance from farms to retail store outlets. Our third aim is to enhance collaborations among federal, state and academic stakeholders with respect to foodborne disease surveillance and outbreak investigation. Collaborating partners in this project include FDA-CVM, the California Department of Food and Agriculture (CDFA), the California Department of Public Health (CDPH), and UC Davis WIFSS and SVM. By accomplishing these specific outcomes the NARMS system will further expand its surveillance of antibiotic resistant bacteria in retail meat and facilitate collaboration among federal and state agencies and academia in detection, surveillance, and investigation of foodborne outbreaks associated with antibiotic resistance.