Preclinical Imaging Shared Resource

临床前成像共享资源

• 批准号: 10491812
• 负责人: Heather Colleen Jensen Smith
• 金额: $ 1.42万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10491812
• 关键词: 3-Dimensional; 4D Imaging; Anatomy; Animal Behavior; Animal Model; Animals; Atlases; Basic Science; Biological; Biological Models; Biological Phenomena; Cancer Center; Cancer Center Support Grant; Cancer Patient; Cells; Cessation of life; Clinical; Clinical Trials; Collection; Complex; Consultations; Custom; Data; Data Analyses; Educational workshop; Electron Microscopy; Equipment; Etiology; Experimental Designs; Foundations; Funding; Funding Opportunities; Goals; Grant; IACUC; Image; Imaging Techniques; Imaging technology; Individual; Influentials; Institution; Life; Magnetic Resonance Imaging; Malignant Neoplasms; Microscopy; Modality; Molecular; Morphology; Multimodal Imaging; Mus; Onset of illness; Operative Surgical Procedures; Optics; Organ; Organoids; Physiological; Reporter; Research; Research Personnel; Research Proposals; Research Training; Resistance; Resolution; Resource Sharing; Resources; Sampling; Site; Specialist; System; Techniques; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Training; Training Programs; Ultrasonography; United States National Institutes of Health; Whole Organism; X-Ray Computed Tomography; animal imaging; animal tissue; anticancer research; base; clinical imaging; contrast imaging; cost effective; data integration; digital; experience; image guided; imaging capabilities; imaging facilities; imaging modality; in vivo; in vivo imaging system; innovation; insight; instrumentation; intravital imaging; intravital microscopy; irradiation; multidimensional data; outreach; photoacoustic imaging; preclinical imaging; prospective; repository; response; single photon emission computed tomography; submicron; tool; translational cancer research; ultrasound; web site

PROJECT SUMMARY: PRECLINICAL IMAGING SHARED RESOURCE Understanding complex biological phenomena often governing life and death in cancer patients requires analysis and understanding at the single cell or molecular level, as well as a whole organ and/or systemic level approach/understanding. The Preclinical Imaging Center (PIC) at UNMC houses specialized imaging equipment from three complementary research cores (IVIS Optical Imaging, Ultrasonic Imaging, Multiphoton Intravital and Tissue Imaging) to allow PFBCC researchers to readily transition across imaging modalities and generate multi-level, multi-dimensional datasets. When used individually, or in combination to maximize impact and rigor, the PIC provides researchers with all the tools necessary to evaluate critical morphological, physiological, and/or cellular changes responsible for disease onset, progression, and response/resistance across a diverse set of biological samples. Within the PIC, innovative, comprehensive, and cost-effective imaging resources pertinent to a diverse collection of cancer studies are available to BCC researchers. The IVIS Optical Imaging Core (IVIS Spectrum In Vivo Imaging System) provides high throughput 2D and 3D imaging of both bioluminescent and fluorescent reporters in live animals that can be evaluated in an anatomical context using the Digital Mouse Atlas or co- registered with MRI or CT images. The Ultrasonic Imaging Core (US) houses multiple ultrasonic and photoacoustic imaging technologies allowing researchers to perform basic ultrasound, contrast imaging, Doppler, 3D and 4D imaging, and photoacoustic imaging in live animals. The Multiphoton Intravital and Tissue Imaging Core (MITI) combines intravital microscopy and multiphoton excitation/imaging to provide both high- resolution (submicron) and whole organ/tissue imaging in live animals, tissue explants, organoids, and various fixed samples in 2D and 3D. The PIC is a cancer-focused research center housed within a larger Small Animal Imaging Facility (SAIF) providing BCC researchers with access to additional small animal imaging resources including, small animal MRI, SPECT/CT, and Optical Surgical Navigation. Additional affiliated research cores (Advanced Microscopy, Electron Microscopy, Image Guided Irradiation, Animal Behavior) provide complementary resources further extending the research repertoire and data integration capabilities of our researchers. In addition to its instrumentation, by providing custom-tailored, one-on-one training, and expertise/experience in experimental design, image acquisition, and data analysis, the PIC provides cancer researchers with all the tools necessary to achieve new insights into the most daunting of research challenges. Furthermore, with the shared resources available at the PIC, BCC researchers have the exceptional opportunity to actively integrate imaging data obtained from various translational animal models using multiple imaging technologies which both parallel, and expand, clinical imaging modalities available on campus.

项目总结：临床前成像共享资源 了解复杂的生物学现象往往决定着癌症患者的生死， 在单细胞或分子水平以及整个器官和/或系统水平上的分析和理解 理解/理解。UNMC的临床前成像中心（PIC）拥有专门的成像设备， 来自三个互补研究核心（IVIS光学成像，超声成像，多光子成像）的设备 活体和组织成像），使PFBCC研究人员能够轻松地转换成像模式， 生成多层次、多维数据集。当单独使用或组合使用时， 和严谨性，PIC为研究人员提供了所有必要的工具，以评估关键的形态， 导致疾病发作、进展和应答/抗性的生理和/或细胞变化 在一组不同的生物样本中。 在PIC内，创新、全面和具有成本效益的成像资源与各种 BCC研究人员可以获得癌症研究的集合。IVIS光学成像核心（IVIS光谱 体内成像系统）提供生物发光和荧光的高通量2D和3D成像 报告人在活的动物，可以评估在解剖学背景下使用数字鼠标图谱或合作， 与MRI或CT图像配准。超声成像核心（US）包含多个超声和 光声成像技术允许研究人员进行基本的超声，对比成像， 多普勒、3D和4D成像以及活体动物的光声成像。活体和组织多光子 成像核心（MITI）结合了活体显微镜和多光子激发/成像， 分辨率（亚微米）和活体动物、组织外植体、类器官和各种 在2D和3D中固定采样。 PIC是一个以癌症为重点的研究中心，位于一个更大的小动物成像设施（SAIF）内。 为BCC研究人员提供额外的小动物成像资源，包括小动物 MRI、SPECT/CT和光学手术导航。其他附属研究核心（高级显微镜， 电子显微镜、图像引导照射、动物行为）进一步提供补充资源 扩展我们研究人员的研究曲目和数据集成能力。除了其 仪器，通过提供定制的，一对一的培训，和专业知识/经验，在实验 PIC集设计、图像采集和数据分析于一体，为癌症研究人员提供了所有必要的工具 以实现对最艰巨的研究挑战的新见解。此外，由于资源共享 在PIC，BCC研究人员有机会积极整合成像数据 使用多种成像技术从各种平移动物模型获得，所述成像技术既平行， 扩展临床成像模式。