Preclinical Imaging Shared Resource

项目摘要：临床前影像共享资源 了解控制癌症患者生死的复杂生物现象需要 在单个细胞或分子水平以及整个器官和/或系统水平上的分析和理解 接近/理解。UNMC的临床前成像中心(PIC)拥有专门的成像设备 来自三个互补研究核心的设备(IVIS光学成像、超声成像、多光子 活体内和组织成像)，使PFBCC研究人员能够轻松地在不同成像模式之间过渡，并 生成多级别、多维数据集。单独使用或组合使用以最大限度地发挥影响 和严谨性，PIC为研究人员提供了评估关键形态的所有必要工具， 导致疾病发生、发展和反应/抵抗的生理和/或细胞变化 在一组不同的生物样本上。 在PIC内，创新、全面且经济高效的映像资源与各种 BCC研究人员可以获得癌症研究的集合。IVIS光学成像核心(IVIS Spectrum 在活体成像系统中)提供生物发光和荧光的高通量2D和3D成像 在活体动物中的记者，可以使用数字鼠标地图集或联合- 注册了MRI或CT图像。超声成像核心(US)包含多个超声波和 光声成像技术使研究人员能够进行基础超声、对比成像、 多普勒、3D和4D成像，以及活体动物的光声成像。生命和组织中的多光子 成像核心(MITI)结合了活体显微镜和多光子激发/成像技术，提供了两种高性能的 活体动物、组织外植体、器官和各种组织的分辨率(亚微米)和整个器官/组织成像 修复了2D和3D中的采样。 PIC是一个专注于癌症的研究中心，位于一个更大的小动物成像设施(SAIF)内 为BCC研究人员提供访问其他小动物成像资源的途径，包括小动物 MRI、SPECT/CT和光学外科导航。其他附属研究核心(高级显微镜、 电子显微镜、图像引导照射、动物行为)提供了进一步的补充资源 扩展我们研究人员的研究曲目和数据集成能力。除了它的 仪器，通过提供定制的一对一培训和实验方面的专业知识/经验 从设计、图像采集到数据分析，PIC为癌症研究人员提供了所有必要的工具 以获得对最艰巨的研究挑战的新见解。此外，通过共享资源， 在PIC提供，BCC研究人员有特殊的机会积极整合成像数据 从使用多种成像技术的各种翻译动物模型中获得，这些成像技术既平行，又 扩大校园内提供的临床影像设备。

PROJECT SUMMARY: PRECLINICAL IMAGING SHARED RESOURCE Understanding complex biological phenomena often governing life and death in cancer patients requires analysis and understanding at the single cell or molecular level, as well as a whole organ and/or systemic level approach/understanding. The Preclinical Imaging Center (PIC) at UNMC houses specialized imaging equipment from three complementary research cores (IVIS Optical Imaging, Ultrasonic Imaging, Multiphoton Intravital and Tissue Imaging) to allow PFBCC researchers to readily transition across imaging modalities and generate multi-level, multi-dimensional datasets. When used individually, or in combination to maximize impact and rigor, the PIC provides researchers with all the tools necessary to evaluate critical morphological, physiological, and/or cellular changes responsible for disease onset, progression, and response/resistance across a diverse set of biological samples. Within the PIC, innovative, comprehensive, and cost-effective imaging resources pertinent to a diverse collection of cancer studies are available to BCC researchers. The IVIS Optical Imaging Core (IVIS Spectrum In Vivo Imaging System) provides high throughput 2D and 3D imaging of both bioluminescent and fluorescent reporters in live animals that can be evaluated in an anatomical context using the Digital Mouse Atlas or co- registered with MRI or CT images. The Ultrasonic Imaging Core (US) houses multiple ultrasonic and photoacoustic imaging technologies allowing researchers to perform basic ultrasound, contrast imaging, Doppler, 3D and 4D imaging, and photoacoustic imaging in live animals. The Multiphoton Intravital and Tissue Imaging Core (MITI) combines intravital microscopy and multiphoton excitation/imaging to provide both high- resolution (submicron) and whole organ/tissue imaging in live animals, tissue explants, organoids, and various fixed samples in 2D and 3D. The PIC is a cancer-focused research center housed within a larger Small Animal Imaging Facility (SAIF) providing BCC researchers with access to additional small animal imaging resources including, small animal MRI, SPECT/CT, and Optical Surgical Navigation. Additional affiliated research cores (Advanced Microscopy, Electron Microscopy, Image Guided Irradiation, Animal Behavior) provide complementary resources further extending the research repertoire and data integration capabilities of our researchers. In addition to its instrumentation, by providing custom-tailored, one-on-one training, and expertise/experience in experimental design, image acquisition, and data analysis, the PIC provides cancer researchers with all the tools necessary to achieve new insights into the most daunting of research challenges. Furthermore, with the shared resources available at the PIC, BCC researchers have the exceptional opportunity to actively integrate imaging data obtained from various translational animal models using multiple imaging technologies which both parallel, and expand, clinical imaging modalities available on campus.