Biomechanics of Neural Tube Development using Brillouin-OCT Multimodality

使用布里渊-OCT 多模态进行神经管发育的生物力学

• 批准号: 10551412
• 负责人: RICHARD H. FINNELL
• 金额: $ 10.39万
• 依托单位: UNIVERSITY OF HOUSTON
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10551412
• 关键词: 3-Dimensional; Address; Adult; Affect; Automobile Driving; Bilateral; Biology; Biomechanics; Biophotonics; Birth; Brain; Cell Shape; Cells; Cellular biology; Collagen; Complement; Congenital Abnormality; Congenital Heart Defects; Data; Defect; Deposition; Development; Developmental Biology; Diagnosis; Diagnostic; Diet; Dorsal; Elasticity; Embryo; Embryology; Embryonic Development; Embryonic Heart; Environmental Risk Factor; Epithelial; Epithelial Cells; Extracellular Matrix; Failure; Fetus; Folic Acid; Functional Imaging; Future; Gene Expression Profile; Generations; Genes; Genetic; Genetic Models; Goals; Homeostasis; Human; Human Development; Image; Imaging Device; Institution; Knowledge; Label; Life; Light; Maps; Measurement; Mechanics; Mentors; Methodology; Methods; Microscopy; Molecular; Molecular Genetics; Mus; Nature; Nervous system structure; Neural Fold; Neural Tube Closure; Neural Tube Defects; Neural Tube Development; Neural tube; Neurologic; Neurons; Operative Surgical Procedures; Optical Coherence Tomography; Optical Methods; Optics; Outcome; Patients; Pattern; Population; Positioning Attribute; Pregnancy; Prevalence; Prevention; Property; Public Health; Publishing; Research; Research Personnel; Resolution; Signal Pathway; Somites; Spinal Cord; Spinal Dysraphism; Structural Congenital Anomalies; Structural Protein; Structural defect; Structure; Testing; Tissues; Transgenes; Universities; Work; base; cardiogenesis; cell motility; congenital heart disorder; crosslink; design; disability; elastography; experience; imaging approach; imaging modality; improved; in utero; innovation; insight; mechanical stimulus; migration; mouse model; multidisciplinary; multimodality; mutant mouse model; neural patterning; neural plate; neurogenesis; novel; physical process; relating to nervous system; second harmonic; tissue mapping; tool; training opportunity

PROJECT SUMMARY The objective of this proposal is to understand how tissue elasticity regulates neural tube closure (NTC) in the moue embryo. The proposal focuses on developing live imaging approaches to visualize and quantify tissue elasticity with an advanced elastic imaging modality, Reverberant 3D OCE (Rev3D-OCE). By determining how tissue elasticity changes longitudinally through development, I will generate the first elasticity maps of the embryonic neural tube. With this information, I will overlay neural epithelia cell migration patterns to determine if these cells are using durotaxis, a form of cell migration where cells are guided by stiffness gradients, as means to successfully execute neural tube closure. This work is significant because after congenital heart disease, neural tube defects (NTDs) are the most prevalent structural birth defect. While great advances have been made in determining the genetic and environmental causes of NTDs, its persistence in the human population demands that we further elucidate the underlying causes to this detrimental defect. This proposal is also designed to serve as a training opportunity. My future goal is to become a multidisciplinary researcher at an academic institution. Completing the work proposed in this application will give me the opportunity to develop novel optical and live imaging tools, enhance my understanding of embryology and developmental biology, and challenge me to be independent and persistent in my research.

项目摘要 本提案的目的是了解组织弹性如何调节神经管闭合（NTC）， 小鼠胚胎该提案的重点是开发实时成像方法来可视化和量化组织 使用高级弹性成像模式Reverberant 3D OCE（Rev 3D-OCE）进行弹性检查。通过确定如何 组织弹性在发育过程中纵向变化，我将生成第一个弹性图。 胚胎神经管有了这些信息，我将覆盖神经上皮细胞迁移模式，以确定是否 这些细胞使用硬旋转，一种细胞迁移的形式，其中细胞由刚度梯度引导， 成功地进行神经管闭合这项工作意义重大，因为先天性心脏病后， 神经管缺陷（NTD）是最普遍的结构性出生缺陷。虽然已经取得了很大的进步 在确定NTD的遗传和环境原因时，其在人群中的持久性需要 我们需要进一步阐明这种有害缺陷的根本原因。该提案还旨在服务于 作为一个培训机会。我未来的目标是成为一个学术机构的多学科研究人员。 完成本申请中提出的工作将使我有机会开发新颖的光学和实时 成像工具，增强了我对胚胎学和发育生物学的理解，并挑战我成为 独立和坚持我的研究。

项目成果

Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.

使用Brillouin显微镜在活胚中神经管闭合的延时机械成像。

• DOI: 10.1038/s41598-023-27456-z
• 发表时间: 2023-01-06
• 期刊: Scientific reports
• 影响因子: 4.6

Actuation enhances patterning in human neural tube organoids.

• DOI: 10.1038/s41467-021-22952-0
• 发表时间: 2021-05-27
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Abdel Fattah AR;Daza B;Rustandi G;Berrocal-Rubio MÁ;Gorissen B;Poovathingal S;Davie K;Barrasa-Fano J;Cóndor M;Cao X;Rosenzweig DH;Lei Y;Finnell R;Verfaillie C;Sampaolesi M;Dedecker P;Van Oosterwyck H;Aerts S;Ranga A
• 通讯作者: Ranga A