Center for C elegans Anatomy

项目摘要 线虫，秀丽线虫，是生物医学研究中最常用的模式生物之一 由于其优良的遗传学和可接近的细胞生物学。这种非常小而简单的动物的完整基因组 有很好的注解，并且有一个大型的遗传工具包可供其研究。大多数成人解剖学都是 从连续薄片的电子显微镜(EM)显微照片重建。然而，EM技术和 EM显微图像的解释是具有挑战性的，并且经常依赖于专业知识。此外， 用于线虫原始解剖描述的大部分EM数据不容易获取，除非 通过这笔赠款的支持。线虫解剖中心的长期目标是 为研究界提供有关线虫的卓越解剖学知识和数据来源，并帮助 对研究生和博士后进行EM艺术方面的培训。我们让解剖学数据对于新手来说很容易理解， 为那些寻求详细信息的人提供全面的信息，并可通过我们的在线平台免费获取。这个 中心通过1)以以下形式发布关于线虫解剖的详细信息： 带注释的EM和光学显微照片、图形插图和相应的文本，作为解剖手册 在我们的WormAtlas网站上。在接下来的项目期间，我们将在线虫上创建新的内容 胶质细胞的细胞器结构和详细描述。我们还将试点引入其他生物医学 将相关的线虫物种纳入我们的解剖手册。2)我们托管并提供最大的收藏 通过我们的在线WormImage数据库获取EM数据。这些数据大部分是以照片的形式收集的 底片和印刷品，然后由中心数字化和注解。在即将到来的时期内，我们 将增加线虫和其他线虫物种的新数据集。此外，我们将与 国家超级计算应用中心将基于几个参考EM开发3D模型 数据集。3)该中心定期为学生和博士后提供现代EM方法的实践培训 合伙人。该中心还测试和开发了针对线虫的新的EM方法。最后，我们还进行了新的 对处于关键发育阶段的野生动物解剖进行EM调查，以补充地图集，并填补 我们对线虫解剖的常识。

Project Summary The nematode, Caenorhabditis elegans, is among the most utilized model organisms for biomedical research due to its excellent genetics and accessible cell biology. The complete genome of this very small simple animal is well annotated and a large genetic toolkit is available for its study. Most of the adult anatomy has been reconstructed from electron microscopy (EM) micrographs of serial thin sections. However, EM techniques and interpretation of EM micrographs are challenging and frequently rely on specialized expertise. Furthermore, much of the EM data used for the original anatomical descriptions of C. elegans are not easily accessed except through the support of this grant. The long-term objective for the Center for C. elegans Anatomy is to be the preeminent source of anatomical knowledge and data on C. elegans for the research community and to help train graduate students and postdocs in the art of EM. We make anatomical data easily interpretable for novices, comprehensive for those seeking detailed information, and freely available through our online platforms. The Center does this by 1) publishing detailed information regarding the anatomy of the nematode in the form of annotated EM and light micrographs, graphical illustrations, and corresponding text as anatomical handbooks on our WormAtlas website. During the upcoming project period we will create new content on C. elegans organelle structure and detailed descriptions of glia. We will also pilot the introduction of other biomedically relevant nematode species into our anatomical handbooks. 2) We host and make available the largest collection of EM data through our online WormImage database. Most of these data were collected as photographic negatives and prints and subsequently digitized and annotated by the Center. During the upcoming period we will add new data sets of both C. elegans and other nematode species. Furthermore, we will collaborate with the National Center for Supercomputing Applications to develop 3D models based on several reference EM datasets. 3) The Center regularly provides practical training in modern EM methods for students and postdoctoral associates. The Center also tests and develops new EM methods for C. elegans. Finally, we also conduct new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill in gaps in our general knowledge of C. elegans anatomy.