CENTER FOR CAENORHABDITIS ELEGANS ANATOMY

秀丽隐杆线虫解剖中心

• 批准号: 6262770
• 负责人: DAVID H. HALL
• 金额: $ 47.84万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6262770
• 关键词: Caenorhabditis elegans; DVD /CD ROM; Internet; bioimaging /biomedical imaging; biological models; cell biology; cell type; electron microscopy; genome; growth /development; handbook; helminthic antigen; immunocytochemistry; information dissemination; literature survey; microorganism resource /registry; morphology; mutant; protein localization; sectioning; technology /technique development; transmission electron microscopy

DESCRIPTION (Adapted from the applicant's abstract): The nematode, C. elegans, has been identified as a "high connectivity" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods in C. elegans, particularly to: 1) study cellular changes during normal development and in mutant animals; and 2) conduct EM-immunocytochemical studies to localize antigens at the ultrastructural level. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is: 3) publishing more detailed information regarding the anatomy of the nematode in the form of annotated electron micrographs. The information is available on CD-ROMs, and will soon be on the World Wide Web, and possibly in book form. Where necessary, the Center is: 4) testing new EM methods for the nematode, and conducting additional EM surveys of the wild-type anatomy at several developmental stages to supplement the Atlas, and particularly to fill gaps in our general knowledge. The Center also provides: 5) training in transmission EM methods so that more C. elegans laboratories can conduct their own EM studies. This Pre-Resource (R24) application will continue to build the capabilities of the Center over a five year period; it was formally opened in February 1998.

描述（改编自申请人的摘要）：线虫，C。 线虫，已被确定为细胞的“高连通性”动物， 发展 这种非常小，简单的动物已被广泛采用， 实验研究，由于其优良的遗传学和可访问的细胞生物学。 该蠕虫的完整基因组已经公布，大部分成虫 解剖结构已重建从电子显微照片系列薄 路段 然而，EM技术并没有被C. elegans 社区，和大多数以前的EM数据不容易访问。 C中心。elegans Anatomy专注于扩展EM方法， C. elegans，特别是：1）研究细胞的变化，在正常 发育和突变动物;和2）进行EM免疫细胞化学 在超微结构水平定位抗原的研究。 此类研究 与NIH和NSF资助的实验室密切合作， 以实现他们现有的实验目标。 此外，中心 是：3）公布有关解剖结构的更详细的信息， 线虫的电子显微照片。 信息 可在CD-ROM上获得，并将很快在万维网上，并可能在 书的形式。 在必要时，该中心将：4）测试新的EM方法， 线虫，并进行额外的EM调查的野生型解剖， 几个发展阶段，以补充地图集，特别是填补 在我们的常识中的空白。 该中心还提供：5）培训 透射电镜方法，使更多的C. elegans实验室可以进行 自己的EM研究。 此预资源（R24）应用程序将继续构建以下功能 该中心历时五年，于1998年2月正式开放。