CENTER FOR CAENORHABDITIS ELEGANS ANATOMY

描述(改编自申请人的摘要)：线虫C. 秀丽，已被确定为一种细胞高度连接的动物 发展。这种非常小、简单的动物已经被广泛地收养 由于其优良的遗传学和可接近的细胞生物学而进行实验研究。 这种蠕虫的完整基因组已经公布，大多数成虫 从连续薄片的电子显微照片中重建了解剖结构 横断面。然而，EM技术并没有被线虫广泛使用 社区，而且大多数以前的EM数据都不容易访问。 线虫解剖中心专注于将EM方法扩展到 线虫，特别是为了：1)研究正常情况下细胞的变化 发育和突变动物；2)进行EM免疫细胞化学 在超微结构水平上定位抗原的研究。这类研究是 是与NIH和NSF资助的实验室密切合作进行的， 以实现他们现有的实验目标。此外，该中心 是：3)发布更详细的关于大脑解剖的信息 附注电子显微照片形式的线虫。这些信息是 以光盘形式提供，不久将在万维网上提供，并可能在#年 书的形式。必要时，该中心将：4)测试新的EM方法 线虫，并对野生类型的解剖进行额外的EM调查， 补充地图集的几个发展阶段，特别是填补 我们常识中的空白。该中心还提供：5)培训 传播EM方法，以便更多的线虫实验室能够进行他们的 自己的新兴市场研究。 此Pre-Resource(R24)应用程序将继续构建以下功能 该中心为期五年，于1998年2月正式启用。

DESCRIPTION (Adapted from the applicant's abstract): The nematode, C. elegans, has been identified as a "high connectivity" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods in C. elegans, particularly to: 1) study cellular changes during normal development and in mutant animals; and 2) conduct EM-immunocytochemical studies to localize antigens at the ultrastructural level. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is: 3) publishing more detailed information regarding the anatomy of the nematode in the form of annotated electron micrographs. The information is available on CD-ROMs, and will soon be on the World Wide Web, and possibly in book form. Where necessary, the Center is: 4) testing new EM methods for the nematode, and conducting additional EM surveys of the wild-type anatomy at several developmental stages to supplement the Atlas, and particularly to fill gaps in our general knowledge. The Center also provides: 5) training in transmission EM methods so that more C. elegans laboratories can conduct their own EM studies. This Pre-Resource (R24) application will continue to build the capabilities of the Center over a five year period; it was formally opened in February 1998.