Center for Caenorhabditis Elegans Anatomy

摘要 线虫，秀丽隐杆线虫，已被确定为“高”， 连接”动物细胞的发展。这种简单的小动物 由于其优良的遗传学特性， 细胞生物学蠕虫的完整基因组已经公布， 大多数成人解剖结构都是通过电子技术重建的 连续薄切片的显微照片。然而，EM技术并不广泛地应用。 利用C. elegans社区，大多数以前的EM数据不容易 除了通过这种资助之外，C中心。elegans 《解剖学》专注于1）发布有关 以注释的TEM和光学显微照片的形式对线虫进行解剖， 可在我们的两个网站WormAtlas（基于文本）和WormImage（图像 数据库），并通过ftp，在硬盘驱动器上，并在书籍的形式作为C。秀丽隐杆线虫 该中心2）为学生提供现代EM方法的实践培训 和博士后的和3）测试新的EM方法为C。优雅我们还进行 在关键发育阶段对野生型解剖进行新的EM调查， 补充地图集，并填补我们一般知识的空白。

ABSTRACT The nematode, Caenorhabditis elegans, has been identified as a “high connectivity” animal for cellular development. This very small simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed except through the support of this grant. The Center for C. elegans Anatomy concentrates on 1) publishing detailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs, available on our two websites WormAtlas (text-based) and WormImage (image database), and by ftp, on hard drives, and in book form as the C. elegans Atlas. The Center is 2) providing practical training in modern EM methods for students and postdoc's and 3) testing new EM methods for C. elegans. We also conduct new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill in gaps in our general knowledge.